...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections Pages Duchenne muscular... around 30 years of age. Despite the amount of research conducted on DMD, there is still no good treatment...it difficult to deliver via gene therapy, so researchers have set their sights on another approach. Since...oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing approaches...CRISPR-mediated exon skipping approach in mice, DMD researchers are very excited. This approach may also be applicable...

...skills required Research skills The ability to think critically and carry out in-depth research is applicable...career, do your research Once you have identified possible careers, the next step is to research what each ...Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting Lab Manager...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy...include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor Lab Manager...Before you begin to apply for a job, it is useful to search online job websites to see what requirements or...Once you’ve assessed your skill set and done your research, it’s time to start sending out applications. ...

... CRISPR technologies available to the academic research community. At Addgene, we’ve helped distribute...provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus...proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically Inspired...can make lentivirus production frustrating - a researcher can complete an entire lentiviral production ...receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation...reagents from Addgene can greatly expedite a researcher's ability to make discoveries." If you ever run...plasmids to create your own lentivirus stocks. Our research scientists have provided their standard lentivirus...

...focused on translational research is encouraging. Patients are key to the research, but they are also the...observations regarding what makes this group of researchers special: 1. Stem cell scientists care deeply ...Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about... on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease; and Kevin...the reason this research is happening. Stem cells scientists have certainly not lost site of that. 3. Stem...acquisition is not a rate-limiting step in stem cell research. People told me they couldn't have done their ... to helping the stem cell community with their research, I think I can speak for Addgene when I say that...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...work with Addgenie writers to help share their research, tools, and knowledge. Guest authors The Codon... Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew...NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A...blog this year! Your contributions have helped researchers around the globe find and share knowledge, resources...

...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...also activate the optogenetic accuators. The researchers took a FRET reporter and adapted it into a BRET...

...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...newly available PRESTO-TANGO kit which allows researchers to interrogate the druggable human GPCR-ome using...utility of this platform for drug discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly...-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. ...

...various light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light...position to activate gene transcription. The researchers tested a variety of combinations to optimize ...pattern during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene...cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after ...cycled on-off-on. Using a slit photomask, the researchers also demonstrated the ability to spatially control...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...

...Addgene to accelerate their research. Read on to learn how these researchers plan to use plasmids from ...Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD ...from Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO)... (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals and Patient... Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins assemble into... a professor at the University of Buffalo. His research focuses on understanding the structural determinants...

...whole-genome sequencing becomes more relevant (Garcillán-Barcia et al., 2023). In this post, we’ve grouped...https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F...experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other molecules...tend to be small and poorly studied, but some researchers have suggested they may have relevance to human...natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid tools...