We narrowed to 904 results for: CAN

...is covalent, so free dye can be washed away. In addition, labeling contrast can be improved using disulfide-containing...reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they cannot cross cell...the first fluorophore (the donor) is excited, it can transfer energy directly via resonance to the second...30-100 Å, depending on the probes). In fact, FRET can be used to derive a precise physical distance between... fluorophore labeled and that these fluorophores can undergo FRET when they are near each other. How do...biomolecules each containing multiple binding crevices that can nonspecifically soak up your fluorophore package....delivered to the wrong address in a different city, you can still develop a successful FRET-based assay since...

...plasmids are more stable, can be easily cloned in different bacteria, and can be transferred to various...broad host range plasmids can be used to create recombinant vectors that can replicate in different bacteria...recombinant vectors can be modified further to increase the range of hosts in which proteins can be expressed...natural plasmids and how they function in nature can inform us on how to create better recombinant vectors...in plasmids in nature, and provide tips on how we can harness those features to create broad host range...determined by the different hosts that a plasmid can transfer to, replicate in, and persist in. Some plasmids... range, while others have a broad host range and can replicate and stably maintain the genes they carry...

...Nucleases can also be used to engineer knockout mice. ZFN, TALEN, and CRISPR/Cas9 systems can all be targeted...gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce point...and outbred strains Mouse strains used in research can fall under one of two broad classifications: inbred...Health (NIH) Swiss. Genetically engineered mice Mice can be engineered by inserting a targeting vector containing...with a viral vector containing the gene construct can be used to insert the gene of interest into a zygote...double-stranded DNA breaks, such as ZFN and TALEN, can used to engineer knockin mice. Co-injection of a ...cell types in which the conditional recombination can occur based on the cells in which Cre is expressed...

... available, and can be submitted via email for cell lines profiled in the lab. You can browse entries ... know if your lines may be especially vulnerable can be very informative. Spoiler alert: HeLa is the most...resource.  This is a labor-intensive project; scripts can be used in some cases, but much of this work has ...synonyms will all get you to the right entry.) You can look up the cell lines you are currently or planning...decide which line is best for your experiments. You can also use the Cellosaurus STR similarity search tool...lines similar to your STR profiles of interest. You can filter CLASTR results by minimal number of markers...and similarity score match. This tool’s algorithm can be adjusted based on query, reference, and shared...

...that can be used for specific purposes. For instance, on our Optogenetics Collection page, you can find...coming out in a myriad of journals every week, it can sometimes be difficult for great work to gain prominence...noise. Scientists have a variety of metrics they can use to evaluate the impact of their work (H-index...our plasmid pages and provide a citation that you can put straight into your reference section. Why do...been used in your system. Sophisticated search You can search for specific plasmids on our website using...search bar found at the top right of every page. You can search for plasmids by name, PI, Addgene ID number... initial search might give you many results, you can narrow your search results using the filters found...

...applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce...and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing mammalian...vectors are deficient in the E1 and E3 regions and can accommodate up to 8.2 kb of transgene DNA. The second...(HC-Ad__ are devoid of viral genes and therefore can accommodate up to 36 kb. Gutless Ad maintain only...Importantly, recombination-competent adenovirus (RCA) can be generated if the E1 gene from the packaging cell... detailed protocol for production of Gutless AdV can be found in reference 3. Production, amplification...generation AdV     AdV Production in HEK293 cells can be broken down into 5 key steps: Construction of...

...us, CRISPR is an incredibly versatile system that can be tweaked to target ncRNA. CRISPR/Cas9 techniques...small deletions and insertions in the DNA sequence can completely disrupt expression of the transcribed ...(Ho et al. 2014). To overcome this, paired gRNAs can be used to target specific sizes of a DNA fragment... et al. 2021). Alternatively, off-target effects can be minimized by disrupting lncRNA regulatory elements...out the majority of the lncRNA locus, scientists can mutate the transcription start site, splice sites...Mutating splice sites is particularly effective as it can result in the retention of introns or the removal...generates double-strand breaks, a new DNA sequence can be knocked-in, or introduced, into the break sites...

...cell. However, much knowledge about functionality can be gained by understanding the location and transport...and transport between healthy and diseased states can also provide interesting insights into disease mechanisms...interest is to make it detectable. Visualization can be achieved using fluorescent microscopy - even in... in living cells and whole organisms. You can take advantage of the many Fluorescent Proteins (FP) available...the internal organization of a cell. This mapping can be done by using antibodies targeted to the marker...reach their intracellular targets. Therefore, it can be beneficial to employ well-characterized marker...Institute) and Mark Prescott (Monash University). You can find more plasmids for labeling your subcellular ...

...your population? Sometimes this can’t be avoided, but if it can be, you can reduce the family-wise error ...mathematicians say, Type I and Type II errors) and can lose a significant amount of power. Not sure if your data... statistical tests – when to use them, what they can tell you, and how to identify which one is right ...difference of the group means). Unpaired t-tests can also be referred to as two-sample or independent,... with room for more variables and groups. ANOVAs can test three or more groups (two samples and a control...discussed later. One and two-way ANOVAs ANOVA tests can compare either one (one-way ANOVA) or two (two-way... as total crop production, while a one-way ANOVA can test either the effect of temperature or humidity...

...country. This information can be of personal interest to the depositor, but it can also be valuable data ...to parse through, aggregation of this information can provide valuable insight to the most requested plasmid...launched, it will show my data by default. Depositors can download their CSV file from Addgene by following... code is available in the Github repository (you can find the link in the upper right hand corner of the...dashboard). Therefore, users with some knowledge of R can further customize the dashboard to their needs. I...uses genetically encoded fluorescent probes. You can follow him on twitter: @joachimgoedhart.    ...