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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 928 results for: Gene

Showing: 121 - 130 of 928 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...knockout, activate or repress target genes in plants using the same general experimental design principles ...Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027...for targeted modification of known or suspected genes that regulate a desired phenotype. In fact, CRISPR... can also be used to activate or repress target genes in plant cells, respectively. Cas9 expression is...regulate expression of longer RNAs (such as mRNAs for gene expression). Examples of commonly used RNA pol II..., activation and repression of target genes in plants and many of the aforementioned empty gRNA backbones...Agrobacterium tumefaciens as a vehicle to deliver your gene of interest into a target cell line or organism ...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ..."Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja (2017...required for the onset of gene expression. However, it has yet to be determined what gene expression looks like...Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression in Mammals A Match...transport desirable or not desirable? If diffuse gene expression is tolerated, then axonal transport is...Otherwise, using a specific system for controlling gene expression such as Cre-lox, may be sufficient to...tool to use. However, we are starting to see that gene expression really does not take much time at all... normally require second strand synthesis before gene expression begins. ScAAV avoid this rate limiting...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ... to make my first CRISPR/Cas9 gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in ...Targeting different regions of the gene is also useful in case the gene has a weird mutation that you didn...each step of performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic...Schematic overview of CRISPR/Cas9 gene editing process. See below for details. The entire process from start...what level of expression was the most efficient for gene editing. A virus encoding GFP plus a gRNA targeting...GFP gRNA, will edit out the virally-delivered GFP gene. So, cells with no Cas9 should appear green and ...multiple gRNAs to target different regions of your gene. I used existing BRAF gRNA viruses, but you can ...

  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...especially when fused to effector proteins for gene activation or gene editing. The more recently identified Cas12f...SEND for gene transfer with pseudotyped viral particles, expanding options for CRISPR or other gene delivery...editing systems improve gene editing performance at therapeutically relevant gene targets and expand the...version (named CasMINI) had the highest activity for gene activation  and base editing, whereas a different..., and fusogen in a modular system for delivering gene editing tools into cells. Image adapted from Segel...editing to correct mutations in a variety of target genes and cell types. Their Nature Biotechnology paper...tyrosinemia, where a pathogenic insertion within the FAH gene was precisely corrected, restoring expression in...

  5. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ... transcription of a target gene, it therefore determines the timing of gene expression and largely defines...synthesis and allowing reporter gene transcription to take place. Addgene also has popular light-regulated...the promoter and ceases repression of the target gene. A simplified lac inducible system is found in many...shock-inducible Hsp70 or Hsp90-derived promoters, in which a gene of choice is only expressed following exposure to...inducible promoters Light is another way to activate gene expression, and two-component systems used in synthetic...promoter pR, preventing expression of a reporter gene. When light is present, YFI is inactive, preventing...experimental system. Leakiness If you’re expressing a gene that may be toxic, it’s important that your inducible...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...the plasmid containing the lethal gene. An example is the ccdB gene system commonly used in Gateway® Cloning...are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that allow for...auxotrophic cell lines), or genes which rescue a lethal phenotype. Resources on the Addgene Blog Read Our Post...well-characterized bacterial lac operon contains a gene called lacZ that encodes for the enzyme β-galactosidase...discovered that deleting a section from the lacZ gene (a mutation called lacZΔM15) creates a non-functional...other methods. Positive selection vectors encode a gene which, when expressed, is lethal to the cell. Cloning...fragments are inserted into an MCS in the center of this gene, disrupting the lethality. This is similar to α-...

  7. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...number of genes involved in cardiovascular and metabolic diseases. We are knocking out genes that have...and find several mutations in the same gene or in multiple genes, all of which seem to contribute to a ...versions of 15 different disease-associated genes. Addgene spoke to Musunuru about how the new kit works, ...scenarios, you can either knock out a gene or knock in a mutation. Addgene: How do you use this kit to target... paper, for example, we generated hepatocytes to examine the effect of a gene on cholesterol metabolism...They do that by studying patients to uncover new gene variants associated with conditions of interest,...be advantageous if you are trying to knock out a gene. One can also exploit homology-directed repair by...

  8. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...used in the lab, the transposase gene is provided in trans so that a gene of interest can be inserted between... 2021).  Gene transfer Transposons are an alternative to viral vector delivery of transgenes, like for...response and are also easier and cheaper to generate. Gene disruption due to integration can occur for...several different applications (mutagenesis screens, transgenic animals, gene transfer). The discovery of ...bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids to the bacteria... genome. In humans, if a transposon jumps into a gene, it could be mutagenic and result in human disease... and mechanism of replication. They contain two genes: gag and pol. The pol polyprotein encodes the reverse...

  9. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...to investigate the roles of specific genes or to knock down genes with potentially harmful mutations in...target gene but have a lot of off-target effects. However, this is a common issue with many gene silencing...this could be applied to. When it comes to altering gene expression, CRISPR technologies have gradually taken...) is, as the name might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark discovery...focusing on shRNA, one of the most popular choices for gene silencing. RNA interference RNAi is a useful tool... in therapeutic settings. Silencing of a target gene is achieved through the delivery of a double-stranded... these tools. Next time you need to knock down a gene, maybe you’ll consider using RNAi! References and...

  10. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...specific genes are signified with a minus sign (-) as is typically standard; just having the gene listed... of the more common genetic changes found in E. coli strains. Table 1: Common gene mutations found in ...carefully selected the optimal promoter for your gene of interest, painstakingly cloned into the perfect...backbone, made sure to add the right tags to your gene, and may have even put a fluorescent protein downstream...in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine methylase mutation (GATC...methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed proteins...encodes proteins needed for bacterial conjugation. Genes listed on F´ are wild-type unless indicated otherwise...
Showing: 121 - 130 of 928 results