...positioning of the FP sequence relative to cell compartment targeting sequences, which often have absolute...requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus [3]. ...Additionally, FPs that encode N-glycosylation consensus sequences are modified with the addition of N-glycans [9...using FPs containing two cysteine residues and a sequence intended to localize them to the secretory pathway...cysteine residues and N-glycosylation consensus sequences in popular FPs. This generated a palette of inert...others (5, 6, 7, 10, 11) highlight the very real consequences of misfolded dark FP fusions including erroneous...

...this gap, the Rat Genome Sequencing Project conducted a large scale sequencing and concluded that nearly... fully sequenced by the year 2000, becoming the first plant to have its entire genome sequenced (Koornneff... limited at first, advances in axolotl genome sequencing have vastly expanded axolotl research. One of...of the most significant advances is the sequencing of the entire axolotl genome, revealing an abundance..., R., Weinstock, G., Metzker, M. et al. Genome sequence of the Brown Norway rat yields insights into mammalian...

...the same sequence to flank a transgene (homologous pairs) or used different scrambled sequences to flank...Recombinase-dependent vectors utilize pairs of specific sequences, lox (recognized by Cre) or frt (recognized by...used a lox-STOP-lox (LSL) or frt-STOP-frt (FSF) sequence prior to an ATG start codon to prevent transcription...recognized by Cre, but they are comprised of different sequences (loxp and lox2272) and can not recombine with ...phenomena by analyzing the plasmid and viral DNA sequences and expression profile at multiple steps from ...containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin, K. 2020). In general, we observe...Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity ...

...initiation sequence known as the Species-Independent Translational Sequence (SITS). This sequence bypasses... via DNA sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific ...in series with unique spacer sequences (pMemoryArray) gives a DNA sequence capable of recording 11 bits...shuttled out of the nucleus due to the nuclear export sequence. Treatment with rapamycin induces Cas9(N)-FRB-... the presence of multiple copies of targeting sequences. Scientists have adopted and employed this concept...inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed promoter-selection marker...generating plasmids with varying numbers of repeated sequences or inserts, which can be advantageous for imaging...

...regulatory sequences to allow for expression of multiple gRNAs (i.e. Csy4 cleavage sequence, tRNAs, multiple...No dsDNA cleavage Blunt end 5' overhang PAM sequence NGG TTTV PAM site preserved? Usually Destroyed...multiple promoters to drive crRNA expression and sequences that allow for array processing, i.e. tRNAs or...Lentiviruses carry a (+) strand RNA copy of the DNA sequence, which is a suitable substrate for Cpf1. This ...

...side of the fluorescent protein sequence that is homologous to the sequence into which it is being inserted...Gene editing is the addition of a useful coding sequence (i.e. GFP) into a gene while leaving other features...in one edited allele, or gene copy. The coding sequence for GFP is transcribed and translated along with...the gene of interest, a linker, and the GFP tag sequence to be inserted. Created with BioRender.com. ...

...adapted the native PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach...also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax, to work synergistically...plasmids  Deletion and replacement of long genomic sequences The PEDAR strategy engineered by Wen Xue’s lab...double-strand breaks and deletes the intervening sequence. As with other prime editing tools, the desired...

...gRNA targeting sequences, you can then readily generate your barcoded gRNA library sequences via oligo synthesis...can be tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible...will need is to get the list of effective gRNA sequences targeting your genes of interest. Thanks to the...where NNN and XXX represent the 20-bp gRNA target sequence and its 8-bp barcode, respectively. Once cloned...

...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be... Description Origin of Replication (ORI) DNA sequence which allows initiation of replication within ... be exploited for sequence verification of plasmids.   How is a plasmid constructed in the lab? Due...

...described in this post are engineered to bind RNA sequences derived from Zika virus and, upon binding, produce...stripped down viral genomes containing only the sequences required for viral replication and transcription...that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong (formerly of the Lu Lab... variety of customizable functions to your DNA sequence of interest. Potential applications of the system...