We narrowed to 621 results for: SCR

..., lengthy genetic payloads and insufficient transcription on the array have limited the scalability and...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

..., it's hard to get those words out and onto the screen and transform your work into a paper or a grant... categorized either based on the section of a manuscript they might be found, i.e. introduction, methods...the Addgene blog: Tips for writing scientific manuscripts: literature searching, reading, and organizing...

...adoption of the technology. In 2012, Saito et al. described the first Nano-lantern, a fusion of a mutagenized...hours! The latest enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous...substrate furimazine. In addition to the previously described applications, the enhanced signal of eNL allows...

...27478040. The lung cancer mutant collection is described in this paper. Systematic Functional Interrogation...the genetic pathway reference collections are described in this paper Systematic morphological profiling...  The genetic pathway reference collection is described in this publication We also wrote an extensive...

...editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex genome editing.  ...transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9 Nissim et alTsai...transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9   Xie et al  ...

...expressing cell lines, which can then be used both for screens (using pooled CRISPR knockout libraries) and individual...monoclonal Cas9-expression line prior to performing a screen or even a single gene knockout. Top AAV: pAAV-hSyn-DIO-hM4D... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

...Cell, Musunuru, Chad Cowan and their colleagues describe a much more efficient tool for doing that disease...modeling work in human pluripotent stem cells: a transcription activator-like effector nuclease (TALEN) kit...people unfamiliar with this new TALEN kit, can you describe it for us? Musunuru: TALENs are a type of genome...

...formulation of new knowledge than it is to read a manuscript whose whole purpose is to provide detailed evidence...images, noises and music, spoken words, and any onscreen text. Be aware of the effort the audience is expending...Maybe I needed to employ a visual aid instead of describing something verbally. Sometimes a group was just...

...features with optimal biological performance are described as Click Biology (Howarth, 2025). Figure... Addgene. Addgene Link Click Biology Tag Description Humberg et al. (2025) Split inteins Cysteine-less...  Click Into Biological Building Click Biology describes the search for robust, reliable reactions, using...

...Systems Biology in November, Voigt and Alex Nielsen describe what’s possible when multi-input CRISPR/Cas genetic...many deposits to Addgene over the years. Can you describe the toolbox you are putting together?  Voigt: ... Those will allow you to set the amount of transcriptional and translational activity a cell has and control...

...soCoChR-expressing system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal...the single-cell level. Various soCoChR plasmids described in this work including the soCoChR-GFP plasmid...expression in E. coli. This operon is under the transcriptional control of a strong constitutive promoter (...

...neurons from each class to fully characterize the transcriptome. The first data release for CeNGEN is planned... The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans. To study the role...Addgene In addition to the wealth of open resources described above, there were many plasmid tools presented...

...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...’t give tRNAs much thought. High throughput transcriptome sequencing methods including work done by the...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

...histone modifications, which can influence the transcription of genes by RNA polymerase II, or by the addition...methylcytosine in CpG islands makes  genes less transcriptionally active. The role of epigenetics in studying...of tumor suppressor genes can repress their transcription and inactivate them. Global hypomethylation,...

...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

...sequences that, when bound by transcription factors, can increase a gene’s transcription. Sometimes enhancers ...vivid chromatin picture, CAPTURE 2.0 re-writes the script on gene regulation. References Han, J., Zhang...

...transfer plasmid at 30 °C instead of 37 °C and then screen for ITR recombination with a SmaI or XmaI restriction...duplicated regions like the ITRs. Restriction digest screening for ITR deletion is still necessary. To save time...mL of my AAV transfer plasmid cultures and then screen with SmaI digests. I freeze the bacterial pellet...

...following cloning methodologies have been extensively described in other articles; so if you are interested in... to assemble up to 6 different fragments in a prescribed order. These fragments can be pooled in one tube...homology. You might also consider FastCloning, first described by Chaokun Li and colleagues. Here you simply ...

...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...assembled in vitro using purified components of the transcription and translation machinery. These include ribosomes...

...chromoproteins have a shorter history. In 1987, scientists described an unusual blue-colored protein from the jellyfish...methods. While techniques such as  blue-white screening require X-gal, an expensive exogenously-added ...chromoproteins don’t require other substrates for screening. Thus chromoproteins can be used as markers in...