We narrowed to 640 results for: Son

...mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity...

...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...

...strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-...

... Rachel Leeson...

...stop codons.    New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

...recombination in samples. For a more detailed comparison of these cloning strains, browse the table of...

... light.   Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ...

... Rachel Leeson...

...they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you...

...     Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,...

...Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1...

...she will start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility...

...the gut-brain axis (admittedly for self-serving reasons – I want to understand my obsession with the combination...

...Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene...

...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression...

... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal...

... Lianna Swanson...

...shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins...

...to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and...

...functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward...

...contribution to the field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors...

...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions....

...be more difficult in the virtual format than in-person. You might miss out on the short chats between ...

...takes time away from our research. For all these reasons, the QCRG is delighted to be partnering with Addgene...

...reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies may just not perform as...

...the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo...

... neurons near the target site.” pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV...

...reporters have been developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase...

...the research has been adequately proven to be reasonably safe and effective. We do that through a standardized...

...  Rachel Leeson...

...26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & Hahn, K. (2008...

...that total with the indicated function. One huge reason CRISPR has become such a popular genome editing...

...properties of or draw an amino acid or peptide for some reason. It contains all twenty amino acids plus selenocysteine...

...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,...

... Lianna Swanson...

...monitor with biosensors? You can keep tabs on my personal favorite (the caffeine of the cell!)—ATP. There...

...  Rachel Leeson...

...Haery in 2017 and significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et...

...electroporation of a human, for example, isn’t a reasonable option. Since viruses used in labs are derived...

...mediated by some kind of communication to another person.  In an interview setting, this means persuading...