We narrowed to 812 results for: cha;

...matrix (usually agarose) to pull your negatively charged DNA through the gel. As indicated in the figure...the gel in wells positioned toward the cathode (- charge). When the voltage is applied across the gel, the... the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than ...

... Figure 2. Schematic diagram showing the mechanism of spontaneous reversion during AAV plasmid amplification...phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to...outcome.    References and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction...

...quantifies the likelihood that a result is due to chance vs. the factor that is being experimentally manipulated...) or skewed (see Fig. 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test...trial, for example).   Fig. 3: Question flowchart for choosing an ANOVA/MANOVA statistical test ...

...adapted from Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo...oncogene is one of the reprogramming factors) More Chances of accumulation of harmful mutations More, due ...21434814. PubMed Central PMCID: PMC3161101. 20. Chang, C.J., et al., Production of embryonic and fetal-like...

...Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP...BioMolecular Atlas Program (HuBMAP)  Antibody Characterization Laboratory Cancer Any Data repository...

...covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

...template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions...versions of CRISPR nucleases that make single base changes in the DNA sequence without completely cutting ...

...(old board games and movies can provide great character costumes). All of this costume planning is for...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to...

...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...

...created a microbe advent tree displaying over 25 archaea, bacteria, fungi, and viruses. Final tree with...MicrobeAdventCalendar ornament set (plus more!) It features archaea, bacteria, fungi, viruses, and one microbe ornament...

...generally listed as the amino acid change and not a nucleotide change. The proper way to denote an amino...

... Considering citations to all articles, Anurag Acharya and colleagues report, the percentage of citations...has increasingly moved online, surely times have changed. Altmetrics.org calls to expand our view of what...

...through the air and that’s considered unlikely to change – but the illness has been fatal in 70 percent ... Ebolavirus (EBOV) is a Filovirus, a family characterized by single, linear, negative-sense, single-stranded...

...2.2, and provide an indication of purity versus chaotropic agents as guanidine thiocyanate and guanidine...be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall of Gram negative bacteria...

... Zoom’s default backgrounds all year and want a change? Addgene has you covered. Today we’re releasing...next meeting.     If you’re reading this blog, chances are you are a fan of Addgene or our very cute mascots...

...depression, and now researchers have reported a mechanism based on studies of transgenic mice that helps...brain is protected from stress-induced biochemical changes that could otherwise lead to depression. And there...

...must do science to be happy. What do we have to change to ensure that all scientists can have successful...training too many scientists and some propose radical changes to the way we view the graduate school and postdoc...

...Scientists at North Carolina State, in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the...Powerful New Way to Edit DNA" and "This Protein Could Change Biotech Forever."  David S. Weiss of Emory University...

...many molecular biology methods such as allelic exchange, FRET, and synthetic biology. 7. 25 Work From...them in the cell. This blog post features two mechanisms that cause plasmids to be incompatible. 4. RNA...

...by scientists, which is hosted on our Instagram channel every Sunday. What's planned for the upcoming year...while “manipulate” could mean “worked with” or “changed” to scientists. Keep your message to the main three...

...conference, you may think about sitting amongst a sea of chairs listening to talks all day. But nope, not at the...makes scientific topics both interesting and approachable!   The grand finale of visual impressions was...

...variety of pipette tips. This is part of a cultural change in how we approach waste that needs to be supported...for things like truck bumpers, plastic adirondack chairs, and synthetic turf. I was truly amazed by this...

... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk...

...      In June, the White House honored 13 “Champions of Change,” including our recent guest Drew Endy, in...

...for people in different labs to chat informally about their challenges, successes, and expertise. Who ...

...gills. It attaches there until they mature and changes sex from male to female. Then, it detaches from...16191624. PubMed Central PMCID: PMC1559948. Brusca, Richard C., and Matthew R. Gilligan. "Tongue replacement...

...knockout mice, which is time-consuming, as it only changes one gene at a time. CRISPR-based systems enable...power of these systems to investigate in vivo mechanisms, we targeted two phosphatases, PTPN1 and PTPN2...

...yeast developed by Dr. Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection... McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14...

...otherwise not be able to work on through normal mechanisms. It will take the full breadth of our research...comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15...

...that controls repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the...several weeks to delete or deplete individual genes. Michael Laub’s lab developed a CRISPRi system to downregulate...

...and you will be notified via email. There is no charge for the request process.  Ordering If your request...until you have placed your order. You will only be charged once you have added the prep to your cart and checked...

... cause spinal muscular atrophy, the molecular mechanisms behind the disease are unclear. Dr. Di Schiavi... Dr. Di Schiavi will use Addgene plasmids to characterize smn1 interactions with other genes. He will ...

...homepage recently, you probably noticed some big changes to our site (if you haven’t, go check it out!)....happy and inspired to tackle your next science challenge. If you have any feedback, please contact us and...

.... With your busy schedule, you may not have the chance to stop what you’re doing and make a phone call... Bryan Archambault...

...modified with duplicated enhancer domains and characterized in studies of tobacco, Arabidopsis, and corn...paper at Addgene. Other relevant blog posts 3 Challenges in Plant Synthetic Biology Quick, Versatile Plant...

...inter- and intrachromosomal dynamics, and how they change in response to the cell cycle or other stimuli....intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. Future modifications and applications...

...States - Cambridge, MA to be exact. Today, that changes. Today, Addgene launches a brand new Europe office...scientists together from all over the world to exchange research materials in an easy way. She has a passion...

...Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid...

...types of the basal ganglia. They work via newly-characterized enhancers that enable a whole host of (awesome...AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases...

... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows...