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...analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / .../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation...

...C-terminal region of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...approach and outcomes of the screen for improved genetically-encoded voltage indicators (GEVIs) based on the...kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons... HcKCR2, making it more suitable for in vivo optogenetic use.   Fig. 2: A) HcKCR1 is highly selective...control K+ gradients and can be used as a potent optogenetic silencer. The current work also proposes promising... light-gated potassium channels that mediate optogenetic inhibition. Nat Neurosci 25, 967–974 (2022). ...

...pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory of chemogenetic AAV for activating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name ...AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  AAV5 ...pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype Name 99039 ...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...

... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)...Streamlined Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or ... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... review by the Provider Limited liability Addgene makes it easy Even with all those benefits, we ... handle all the paperwork. Record Keeping: Addgene will let you know exactly how many times your materials...

...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...

... biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins. From considering protein... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary. Throughout the ...months, we’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years...

...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...a full time position in a lab. I worked on the genetics of Wilms Tumour, which is a type of pediatric ...study a Master of Science (MSc) in Human Molecular Genetics to give me an edge over other graduates during...costs. My MSc courses were interesting and luckily genetics is a sector which has been rapidly growing. These...factors left me optimistic that I would find a genetics related role soon after finishing my MSc. The ...Australia, this was a perfect opportunity to work in a genetics lab. As a UK citizen, I was entitled to a Work-Travel...the best outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic...

.../nn.4265.  Additional resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit... al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon...Although a wide range of fluorescent proteins and optogenetic tools exist, humans (like many organisms) are...magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets would enable... molecules like proteins with magnetic fields generally appears to be weak — if they exist at all. Andrew... AsLOV2. After several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants...significant gains! After a few more rounds of mutagenesis, they eventually found a version of AsLOV2 with...

...technique, you first perform a round of PCR to generate DNA fragments with 15-40 bp of homology at their... site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I provides both...Taq perfectly anneal with the topoisomerase I generated overhangs in the backbone. Once combined, the ...processes will require a PCR reaction or two to generate templates for the cloning process. Time spent ...spent actually doing the experiments are also generally comparable so the best way to compare costs is to ...assembly is a highly versatile and efficient way to generate a wide variety of constructs. Similar, Gateway...$20-30 per reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced...

...s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics guide BRAIN initiative plasmid... on the Addgene blog Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors...electric stimulation, drug administration, and optogenetics. Enter neural fibers! The neural fiber tool...features enable the fibers to emit light for optogenetic purposes, measure temperature, study electrophysiology...molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     Fig. 1 - ...the labs looked at previous implementation of optogenetic tools, calcium sensors, and other common neuroscience...for gut implantation. The fibers they ended up generating were 10-15 times more flexible than the brain...

...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical...Archon1 can also be used in conjunction with optogenetic activators and inhibitors that respond to blue... Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Seeding Labs Resources on the Addgene Blog Listen to Additional Addgene Podcasts Learn How You Can Contribute...Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research Resources...Resources on Addgene.org Become a part of the Addgene Community ...Biotechnology, he did his PhD in Plant Breeding, Genetics, and Biotechnology at Michigan State University.... Kwapata now works as a lecturer in molecular genetics and biotechnology for Horticulture and Forest ... recently received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that...

...recently awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Resources on the Addgene Blog Check Out Posts on Synthetic Biology Learn about the Addgene, Seeding Labs...In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University... research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists... biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's...Sciences in Ghana. He is a recent recipient of the Addgene, Seeding Labs Plasmid grant and is working on a...

...Imaging Tools Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent... visualize their activity. In 2019, the first generation of norepinephrine sensors was developed, named...norepinephrine (Feng et al., 2019). But this first generation of tools had limited sensitivity and speed and... models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita et al., 2022). B, Representative...Speed One of the major advantages of this next generation of norepinephrine sensors is their speed. nLightG...GRABNE were more sensitive than nLightR, both generating a measurable signal after electrical stimulation...test point, ten pulses. This is in line with the general findings that red fluorescent proteins tend to ...

...found at help.addgene.org, and the widget is on any page on our regular site, www.addgene.org. We’re excited...comes first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features ... at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the Addgene Community...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a... familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our core values ...answer that you’re looking for, or you just have a general question or concern about the answers we provide...excited to make these new features available to the Addgene community, and we’d love to know what you think...

...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Check out our CRISPR Featured...and designated this system REPAIRv1. Using next-generation sequencing, they confirmed A->I editing and found...cytosine deaminase. Three rounds of rational mutagenesis on the ADAR2DD residues that contact the RNA ...RNA-sequencing, the authors performed rational mutagenesis and identified that a S375A mutation in RESCUE...PMID: 29070703. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. “RNA targeting with...PMID: 28976959. Find plasmids from this paper at Addgene. Abudayyeh, Omar O., et al. "C2c2 is a single-... PMC5127784. Find plasmids from this paper at Addgene. East-Seletsky, Alexandra, et al. "Two distinct...

...Professional Organization Resources on Addgene.org Check out Career's at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting... by some colleagues as the “cross-team guy” at Addgene, an unofficial title that I happily embrace (and...Management Team that steers the organization. As an Addgene vet familiar with the expertise and skills of many...possibly understand every nuance of how kits work at Addgene, but with the help of these people and others, ...existing. Here are a few types of teams that exist at Addgene - team types that could exist anywhere in science..., they must work in sync to get tasks done. At Addgene, the Scientist Team is responsible for guiding ...

...project gives Snapgene great exposure to the community of biological scientists who use Addgene everyday. ... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...small to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate...expertise to our mutual benefit.  Our partnership with SnapGene, for example, allows us to display high-quality...like it going, someone (likely several people at Addgene) had to take the initiative to find a suitable ...outside your comfort zone As part of my position at Addgene I am now involved in Strategic Partnership Management...

... a position on the Addgene Board of Directors. We're excited to watch both Addgene and Sample 6 grow with...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Good Manager from Addgene Executive Director Joanne Kamens Resources on the Addgene Website Check Out...installment in our podcast series, we chat with new Addgene Board Member, Michael Koeris. Dr. Koeris did his...developing? 38:00 - End : Dr. Koeris' role on the Addgene Advisory Board and how he hopes to help young biotech...Bacteriophage-based Diagnostics Additional Resources on the Addgene Blog Check the Other Posts in Our Podcast Series...of our Board of Directors Become a Part of the Addgene Community ...

...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services...calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent...neuronal cell body or the axon (GCaMP6 only). The genetic elements required for Drosophila studies have been...chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe complex brain...Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for organellar...SA, Looger LL. Imaging neuronal activity with genetically encoded calcium indicators. Cold Spring Harbor... Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators. Nature protocols...

...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning...the UK, South Korea, and beyond!   Check Out How Addgene Thinks About Developing Lab Management Software...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your newest resources...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular...

...FOP until a cure is available. Addgene and AJ's Avengers This year Addgene, where my wife Lianna has made...their plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the ...years. Additional Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers...potential FOP cure But, there is HOPE. FOP is a genetic disease typically caused by a single R206H point...receptor ACVR1. ACVR1 triggers appropriate bone regeneration in healthy patients. However, the R206H mutation...helping us fight against FOP in two ways. First, Addgene is distributing ACVR1 Plasmids from a variety of...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...

... KillerRed, a genetically encoded red fluorescent photosensitizer protein that generates ROS when illuminated...16369538. Additional Resources on the Addgene Blog Read Other Optogenetics Blog Posts Sign Up to Receive the...residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The iodine-modifications...the main advantages of MG-2I-dL5** over other genetically encoded photosensitizers is that it works with...cells.  He et al also show that MG-2I-dL5** only generates small amounts of non-specific ROS when exposed...Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable near-infrared photosensitizer. Nature...Chepurnykh, T. V., . . . Lukyanov, K. A. (2005). A genetically encoded photosensitizer. Nature Biotechnology...

...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific..., 2020 to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains...

...distances. For instance, how much force can be generated by conformational changes in proteins? How can...can be addressed using R bodies: simple, force-generating protein machines that can extend and retract ...demonstrated that R bodies can be “tuned” with mutagenesis to extend at different pH values. Using a screen...in a particular region of the R-body. Further mutatgenesis of this region produced R-bodies that could ... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are.... & Pond, F. R. Organization and expression of genetic determinants for synthesis and assembly of type...

...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...Join the Addgene Community ...

...commercialization. Many of the genetic pieces developed over the years were generated by large agricultural companies...you have developed a genetic tool for plant engineering, please share it with Addgene to both build the ...transformation to testing and a lack of well-characterized genetic tools make it challenging to engineer a specific... biotechnologists face is public perception of genetic modification (GM) and synthetic biology. The mistrust...research – this comprehensive understanding of the genetic pieces underpinning these mechanisms – our project...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...and we are excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: ...

...Plasmids 101 eBook Resources on Addgene.org Join the community of Addgene depositors here Check out our...repeating before you can finally dive deep into generating the data you’re excited about. When we were ...intervening five years, inflation has occurred. Here at Addgene, the consumables cost per stab has increased 50%...plasmids. Use repositories Repositories such as Addgene help share materials quickly and cheaply. We save...last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means we virtually always...community engagement with and use of repositories like Addgene provides large-scale savings. How large? Using ...average of 5 hours of hands-on time per plasmid, Addgene, since its founding in 2004, has saved the scientific...

...Fragments generation After you’ve designed your cloning scheme, you’re now ready to generate fragments...Paolo Colombi, a product development scientist at Addgene. Cloning can be quite an arduous process. The PCR...fragments. You can generate the parts of DNA that you want to assemble together in a combination of different...conditions. Purifying DNA fragments Once you’ve generated your DNA fragments, it is always a good habit ...step has to be strategy specific, there are few general parameters that you can adjust: temperature and...and time of incubation, and amount of DNA. As a general rule, try to use an excess of insert compared to...currently the product development scientist at Addgene. In the lab he develops new assays and processes...

...Resources at Addgene Find the ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out...abundance and clonal dynamics over time using next generation sequencing (NGS). The library was developed when... al., 2016).  Bhang deposited the library with Addgene because she wanted to make it as easy as possible...and she is excited to deposit the library with Addgene because it is a “really great way of broadly sharing...sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative new ways you put ...and for providing this powerful resource to the Addgene community! Carrie Bhang is a research investigator...in applying what we have learned about tumor heterogeneity to drug discovery and the development of combination...

...fit in a freezer). Our Addgene celebration was so much fun! Here's the Addgene Leadership team in all ... a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...might have you wondering how many plasmids leave Addgene headquarters every day. That would be an average...our shipping room above), but as Joanne Kamens, Addgene’s executive director, said during our celebratory...it takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on...Addgenies on social media or around the office. @Addgene congrats for 1 million orders. @RothLabUNC accounts... — BryanRoth (@zenbrainest) August 21, 2018 As Addgene grows, we have been able to adapt to the needs ...

...proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor... a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR...adapted for detecting SNPs. Learn more from this Addgene blog post Read the paper in Science Find the...the Cas14 plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that...editing is now possible. Learn more from this Addgene blog post Read the paper in Nature Methods Find...a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in the next What...please let us know! Additional resources on the Addgene blog Download the CRISPR 101 eBook Find our CRISPR...

... plasmids for generating your own libraries are also available in the Sybody Generation Toolbox plasmid...lab found a way around this by using plasmids to generate sybodies, synthetic nanobodies against the spike...blog post.  A rapid selection platform for generating sybodies against membrane proteins Developing...membrane protein of interest using the Sybody Generation Toolbox and commercially available reagents in...libraries (Zimmermann et al., 2018). Find the Sybody Generation Toolbox! Starting with libraries and a biotinylated...relevant plasmids are available in the Sybody Generation Toolbox kit. Find the Seeger lab's SARS-CoV-2... S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation of synthetic nanobodies against delicate proteins...

... Figure 1: Discussing next-generation sequencing at the Addgene bioinformatics journal club! ...and level of general busy-ness will most likely the primary drivers of frequency. At Addgene, we opted to...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...wealth of high-throughput sequencing (HTS) data we generate and maintain. It became clear that while several...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...wandering monologue?  Assuming the feedback is generally positive and the meeting seemed to be of use, ...

...training in a variety of transferable skills. At Addgene we're helping to fill this gap by both helping ...yourself in a creative rut, read on!   Fig 1: Blugene using creative problem-solving in the lab   ...to do this and are still struggling with idea generation, try this thought exercise: Consider the issue...three-ring binder as a starting point, the ideas generated were hardly novel. When told to use a roller skate...that way— but the idea behind the study holds. To generate novel ideas, start with an out-of-the-box concept...option for any meeting in which the goal is to generate unique ideas. Recognize ideas can come any time... had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New Ideas Survey. Anyone...

...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...making this transition. In this installment of the Addgene career guide, we’ll think about what causes this...job candidate for a senior scientist position at Addgene, the skeptical applicant asked if she would partake...of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list of things...the years that one of the things I enjoy about Addgene is that I’ve never been bored. There is always ...someone who coordinates cross-team projects at Addgene, I find that my vocabulary improves on a weekly...fix them, and take a look at these tips from the Addgene blog. You’ll have the opportunity to discover...

...nature18308   Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody...applications. You can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well... acid peptide (EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative...clone used today! Goat, chicken, mouse, or rabbit? Addgene has you covered with monoclonal antibodies to Myc...both cleave after the DVPD sequence. Check out Addgene’s monoclonal antibodies (mouse, rabbit, and chicken... antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential tag issues Tags have...Antibody protocols Additional resources on the Addgene blog Antibodies 101: Epitope availability Plasmids...

...Resources on addgene.org  Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics... our collection, on the Addgene eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en...

...Blotting Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...an IF experiment. What is immunofluorescence? Generally speaking, IF is an application in which antibodies...iBiology). IHC and ICC are essentially just more general terms that can also refer to assays using non-fluorescent... visualize their target. These antibodies are generated against a specific antigen and then conjugated... more common is that secondary antibodies are generally polyclonal, which means that multiple secondary...amplification. Another reason is cost effectiveness. Generating and validating primary antibodies is a time consuming...antibodies that exist and you can begin to see why generating fluorophore-conjugated versions for each primary...

...of sample preparation for high-throughput next generation (NGS) sequencing have prevented laboratories ...As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™ Library Preparation... for NGS-based sequencing and confirmation of Addgene’s large and growing collection of curated plasmids...are using this technique in our partnership with Addgene to sequence large numbers of plasmids. plexWell...does not vary by DNA input amount. plexWell™ + Addgene = high quality NGS-assembled plasmids Because of...information about the presence of non-plasmid DNA or heterogeneous and/or mixed clones present in the original ...original sample. At seqWell, we have worked with Addgene to develop a highly optimized bioinformatics pipeline...

...zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger...being deposited at Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski ... for Synaptic Imaging However, the first two generations of iGluSnFR had limitations: they were not optimal...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement...IPTG induction to express soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal...References and Resources Additional Resources on the Addgene Blog Learn about other Fluorescent Biosensors ...Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors Browse All...

...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages...this multi-pronged attack. Inflammation and tumorigenesis The conference opened with a very interesting... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...addition, it was exciting to see that the use of optogenetic tools is expanding to immunological research....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...

...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...social media as a scientist. Now I write for the @Addgene blog & I'm my lab's resident #scicomm expert. What...using social media as a scientist started with the Addgene blog. I wrote my first blog post out of boredom...person in the lab. When I saw a request for an Addgene guest blogger post about colony PCR, I signed up...and accepted a position to work remotely as an Addgene Science Communication Intern. Developing science... using Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my... this panel, but my consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter...

...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows...ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international ... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...project when purchasing export-controlled materials. Addgene will then apply for the license for distribution...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...creation of improved fluorescent proteins through mutagenesis. Chapter 6 - The future of FRET and fluorescent...Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes. You can follow him...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's...Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids... addgene podcast...

...resources on addgene.org  Antibody GuideRecombinant antibodies available from AddgeneBrowse our recombinant...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...space to give proper credit to. Thankfully, as Addgene's resident blog editor, I could take the needed ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...Designed ankyrin repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

...plasmid-based antibodies Resources on Addgene.org Discover Addgene’s antibody plasmid collection ...antibodies are produced Polyclonal antibodies are a heterogeneous mixture of many antibodies that recognize the... response.   Polyclonal antibodies are generated by injecting an animal with an immunogen, then...weeks later. Created with biorender.com. To generate polyclonal antibodies, an animal (such as a rabbit...might be the full-length protein that you want to generate antibodies against, or a unique peptide sequence...experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher level of...the same species your polyclonal antibody was generated in or a polyclonal isotype control, to account...

...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Listen to the entire Hot Plasmids episode! We generally publish 5-10 Hot Plasmid articles with each of...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

...soon with some brand-new Addgene swag!   Last month, we introduced you to Blugene and Aavery's newest friend...friend, our Addgene antibody mascot, here to help us with our upcoming affinity reagent distribution launch...this form or head over to Twitter to vote on @addgene's Twitter poll. Voting is open until Feb. 22, 2022...winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as...that has led them to their biggest discovery yet: Addgene will soon be distributing affinity reagents. Name...hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral ... four will receive some rockin’ newly designed Addgene swag. Good luck and happy naming!   Fig....