We narrowed to 645 results for: Rest

...was free of agarose and could be digested with restriction enzymes.  Cellulose nucleic acid purification...

...should come as no surprise that researchers are interested in developing these vectors for gene therapy....

...antibiotic resistance using multiple approaches. Major restaurant chains including Chipotle and Panera Bread have...

...Emerging Infectious Disease Laboratories.  She is interested in genome replication and transcription of negative...

...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

...conversation.  It’s tough to come up with new and interesting things to do, feel free to crowdsource! 2. Free...

...have experience with research grants? Are you interested in sustainable community development? Volunteer...

...molecules associated with specific genomic regions of interest in a non-biased manner. To achieve this goal, ...

...However, a key limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate...

...choice for small sample sizes, small regions of interest, or complex sequences such as G-C rich areas, ...

...brightness (such as low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid...

...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...

...Credit: Zhuhao Wu, Weill Cornell Medicine.  Interested in this antibody, but wish it were from a different...

...levels low even if plasmid copy numbers are high, restraining further replication.   Figure 3: Comparison...

...extraneous genetic material to reconstitute the gene of interest, reducing the immunogenicity typically associated...

... Massachusetts General Hospital with research interests in the fields of gene editing and stem cells. ...

...they naturally find people with which they have interests and experiences in common. We often refer to this...

...antigenic peptide sequences, attached to a protein of interest (POI), that facilitate immunohistochemistry (IHC...

...Executive Director of Future of Research. He is interested in advocating for and with junior scientists,...

...works as a healthcare consultant in Boston. He is interested in the business of life sciences and it's impact...

... you can achieve using a CRISPRa. Your gene of interest might already be hitting an upper bound of activation...

... delete the entire gene? Perhaps you are more interested in the c-terminus and would like to target that...

... there would be no viable viruses to study. Interestingly, due to certain high profile problems surrounding...

..., the partial genomes complement each other to restore the full-expression cassette. AAV’s natural ability...

...lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences...

...preferences of the αV subunit and its β partners. Of interest, we demonstrated that anti-integrin αVβ5.9 “essentially...

...Gilmore directly to see if he knows anyone who’s interested in meeting Send out as many emails as possible...

...reads that we studied by deep sequencing, but interestingly (and to our frustration) in many cases we only...

...viral protein production, so researchers are also interested in precisely excising the HIV-1 genome from infected...

...This is actually why a department/institute is interested in you. Your science will define your success...

...promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences effortlessly....

...parts, both of which must be present for a gene of interest to be expressed. These permit exquisite control...

...entire signaling pathways, but what if you’re interested in studying signaling pathways? Here’s where ...

...quantification). But what if you’ve got several interesting protein targets and you want to know if they ...

...making specific knock-out edits in a gene(s) of interest and has an average off-target frequency of 5%....

... aspects) applicable to industry if this path interests you  (e.g. human disease or models of disease,...

...description to watch any sections you’re particularly interested in, but I’d recommend watching the whole livestream...

...the non-profit IMFAHE, Inc. She is particularly interested in breast cancer and metastasis as well as teaching...

...mutagenesis to refine antibody performance. If you are interested in learning more about antibody engineering technologies...

... available. Tailed t-tests If you are solely interested in whether or not two samples are statistically...

...CRISPR-Cas9 kicked off over a decade of frenzied interest in bacterial and archaeal immune systems. During...

...bases. Thus, base editors have an activity window restricted to the stretch of ssDNA created by Cas9 binding...

...factors are directly injected into the organ of interest for in vivo transdifferentiation. Multiple cell...

...respectively. The use of far-red light is of particular interest since blue light has limited applications in vivo...

...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...