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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: ACE

Showing: 241 - 260 of 610 results

  1. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...Korte et al., 2021) for all disposable items and surfaces that come into contact with the rAAV and apply...centrifugation (e.g., iodixanol) using a high-speed ultracentrifuge. During the spin, the virions will form a band...ddPCRAAV Purification by Iodixanol Gradient UltracentrifugationAAV Production in HEK293 Cells...

  2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing.... The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence..., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or ...

  3. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...definitive, but visual identification is a good place to start before taking on more time consuming and...fitness cost yet. However, many fluorescent proteins face similar limitations, and many labs are already using...Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine with hydrophilic...

  4. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of wild-type...produces the phenotype you screened for in the first place. With the proper experimental design and validation...

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ... who need to overexpress and purify proteins can face any number of technical challenges depending on ...protease. A TEV protease cleavage site is frequently placed between two tags being used for two rounds of purification...purification, with the cleavage reaction taking place between column runs. The TEV protease itself, with...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...overexpressing mice spent more time in the risky bright place, driven by their motivation to explore novel environments...treatment in rodents. In this test, an animal is placed into a tank of water with no support from below...attempts to escape, or passive floating on the water surface. The latter reflects a state of behavioral despair...

  7. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...recently moved to our brand new location, with more space to grow and help scientists share resources in new...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments of the...actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly look” that...

  8. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...frequently why many people get into science in the first place. The feeling of camaraderie one gets from a well-functioning...could dread these interactions, but you could also embrace them. Talking about science to non-scientists can...curiosity that led you into science in the first place. Finally, if you still find the idea of not holding...

  9. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...focused on therapeutic strategies that use gene replacement therapy, gene editing, MECP2 reactivation, RNA...RNA editing, RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a...is a strong translational candidate for gene replacement therapy. In 2014, RSRT brought together a strong...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...in the cytosol (instead of on the yeast extracellular surface) which enables tracking of protease activity...fluorescent proteins might not localize to the right places, which can provide misleading information on where...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... Thomas D. Gilmore. "Histone acetyltransferases and histone deacetylases in B-and T-cell development, ...GenBank, but it was useful to present it all in one place to get a sense of the similarities and differences...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... and excision recombination reactions that take place when lambda phage infects bacteria. In vivo, these...reactions described below: The BP Reaction takes place between the attB sites flanking the insert and the...gene expression.     The LR Reaction takes place between the attL sites of the generated entry clone...

  13. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an ...-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in performance...www.janelia.org/open-science/voltron-and-positron.  Both Ace2N-mNeon-A122D and Voltron2 are available as Cre-dependent...

  14. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...conference can be intimidating. And it can be exacerbated when everyone seems to know one another, but ...program to Microbe 2020 in Chicago.  Finding our place in networking and mentoring circles We looked at...this first year. Mentors Amanda Gunn, Bryan Gibb, Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton...

  15. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...science. Facebook is continuing its growth, and while many publishers and researchers have Facebook pages...article, find blogs about the study, or even review Facebook and Twitter comments. On We Share science, video...

  16. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...forensic technique (aka DNA fingerprinting) that can place suspects at the scene of a crime (every human in...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...protein attaches to cell surface receptors, other important binding events take place during the course of...viral envelop and the receptors on the target cell surface. The possible interactions and hence possible targets...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...target site, while Cas12a requires a PAM (Protospacer Adjacent Motif) for target cleavage. This test can...assay. This reaction also contains SSB, strand displacement DNA polymerase, recombinase, and the ssDNA-FQ...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides to a substrate...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...activated patterning of dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27...
Showing: 241 - 260 of 610 results