We narrowed to 516 results for: ATO
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TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....our curated list of biosensors too, as another laboratory may have already created the sensor that you ...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ...
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Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog PostPublishedJuly 18, 2019, 12:55 p.m....open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory. There are two main categories of multiplex ... -
Pooled Library Amplifications
TypeBlog PostPublishedMay 30, 2024, 1:30 p.m....protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library 3,548 250 - 10,000... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....additional protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based...of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers and repressors... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....daytime. They mostly stop emerging at night. An incubator with a light cycle makes for very predictable ... phenotypes, check out the Classical Genetics Simulator website. One interesting thing to know is that... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....in real time! I follow great online science communicators like Phil Plait @BadAstronomer, Emily Graslie...when I was trying to find local informal science educators, and ever since, the State Museum has been the... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....(Figure 1B). The A260/230 ratio can be a good indicator of protein contamination, but a number of other...researchers traditionally use the A260/280 ratio as an indicator of protein contamination. However, this ratio ... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog PostPublishedJuly 16, 2020, 1:15 p.m....University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...four examples of technologies pioneered in his laboratory. Learn more here. Additional resources on the... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....CRISPR/Cas9 Synergistic Activation Mediator) uses an improved activator design that consists of inactive... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....viral vectors) without triggering a destructive inflammatory immune response. At the time, adenoviral vectors...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....Microbiology, National Emerging Infectious Disease Laboratories. She is interested in genome replication and...Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m.... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood. Work... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m....example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....signal inside the tumors was overlaid with the anatomical B-mode images in greyscale. ARG expression...-specific ultrasound signal is in hot and the anatomical B-mode images are in greyscale. (scalebar = 2mm...