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Showing: 241 - 260 of 563 results

  1. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... Flexibility to manage their personal lives to achieve a healthy work-life balance. It is harder to manage...responsibilities, which might include training or teaching others, research, and policy development. Every...

  5. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important factors to consider...months reading and writing a proposal before even touching a pipetteman. Take the time to generate new ideas...

  7. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

  8. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...Glick from SnapGene!  Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

  9. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

  10. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

  11. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ...

  12. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...

  13. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...work. A younger interest in being a writer kept chirping up. The evolutionary perspective brought by studies...with authors and referees. And I am constantly teaching – often explaining a technical piece of research...

  16. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... research, we hope we can help all researchers achieve happiness in their careers regardless of their ...by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why and Where Do I Find One? ...

  17. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate student at...the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC suffer from recurrent...

  18. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translated RNAs appear red. To enable this color switching, TRICK requires the following components: a GFP... specificity and resolution of this system are achieved by using multiple copies of the PP7 and MS2 coat...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...complicated (and again, expensive) the different machines are, and how many regulations are in place to ... on the Addgene Blog Find planarian tools for teaching developmental biology Use the pathways over time...

  20. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...courses on genetic manipulation more hands on While teaching courses about gene therapy and genetic manipulation... on the Addgene Blog Find planarian tools for teaching developmental biology Learn how BioBuilder can...
Showing: 241 - 260 of 563 results