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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 573 results for: CHI;

Showing: 241 - 260 of 573 results

  1. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

  2. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted... ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule tracking...

  3. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...outside the box, be creative, and put your inner child to work. If you have an odd or quirky lab story ...

  4. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...been requested more than 100 times each, thereby achieving the coveted “blue flame” status. We’re proud to...scientific research. As Maria Castro of University of Michigan says, “Addgene has transformed the way in which...

  5. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...replication within a plasmid by recruiting replication machinery proteins Antibiotic Resistance Gene Allows for...

  6. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...seeing early results that suggest our content is reaching more people who need it!  After a few months, ...followed with great discussion and questions. I achieved my goal of helping everyone understand the importance...

  7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ...biomedical researchers and ready access to stably archived large-scale datasets by computational biologists...interest using a simple keyword search. For example, searching the term "breast cancer" returns 40 screens performed...

  10. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... Flexibility to manage their personal lives to achieve a healthy work-life balance. It is harder to manage...responsibilities, which might include training or teaching others, research, and policy development. Every...

  11. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...been incorporated into the host organism, usually achieved with the proper selection marker or antibiotic...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

  12. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...PIs who were previously featured in "9 Tips to Achieve Success in Academia". Important factors to consider...months reading and writing a proposal before even touching a pipetteman. Take the time to generate new ideas...

  13. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...engineered Cas9 variant might work for your experiment. PITChing MMEJ as an Alternative Route for Gene Editing ...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

  14. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...Glick from SnapGene!  Ben Glick is President and Chief Scientist at SnapGene, and also a Professor of Molecular...Genetics and Cell Biology at the University of Chicago. He was an early depositor at Addgene, and conceived...

  17. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural modeling...monomeric green fluorescent protein derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407...

  18. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation 119742  AAV5... AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases Plasmid...

  19. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... examine biosynthetic pathways A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use...-boxes genome-wide. It was designed based on MYC-ChIP-seq data from several MYC-dependent cancer cell ...

  20. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...
Showing: 241 - 260 of 573 results