We narrowed to 659 results for: Clu
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TypeBlog PostPublishedJune 26, 2014, 2:23 p.m....you run a restriction digest on a gel you always include proper controls like uncut DNA and the proper ladder...amplification reaction (as opposed to using the all inclusive service some companies offer), you can purify ...
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Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m....engineering research. A variety of new tools came out including the single base editors, Casilio, CombiGEM, and...2A-Puro (PX459) V2.0: What's not in the name? Not included in this plasmid's name are some additional features... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m....organization runs a wide variety of programs, including fall and spring lecture series, science cafes,... information about SITN: DayCon event website (includes archived content) Science in the News website ... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....Start something (a journal, a science lunch club, a biotech club) Be the head of a group (like a grad student... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m....that introduced the kit in December, GreenGate includes the six most frequently used elements in plant...works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and hope that other ... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog PostPublishedJan. 8, 2014, 3:10 p.m.... lentiviral packaging and expression vectors, including pMD2.G and psPAX2. Actually, these two plasmids...years after they were added to the repository, including beta-catenin reporter M50 Super 8x TOPFlash, scramble... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog PostPublishedMay 17, 2016, 2:30 p.m....over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016...is both sortable and searchable, which can help exclude the data that you are not interested in. You can... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....on previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee ... variety of genome modifications in S. Pombe, including full and partial gene deletion, overexpression... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog PostPublishedJan. 23, 2024, 2:15 p.m....scientists in 88 countries around the world. That included 2008 pooled libraries, 290 antibodies, 18,359 ...it more accessible. Other accessibility updates include a printer-friendly design and alt text on all images... -
Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot
TypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....page, and a lot of the content was either small, cluttered, or hard to find. This made the page look not ... place all of the content above the fold, we decluttered the page elements and allowed our content to ... -
Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....mentoring and applauded the new requirement for PIs to include in their annual NIH progress reports information... research be? The second day of the conference included workshops which were framed in advance by the ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog PostPublishedSept. 15, 2014, 6:05 p.m....met the astronaut Jim Lovell I was speechless (the club of people who have been in space is pretty small...Doudna has had a host of other impressive mentors including Tom Cech, Robert Tijian, Tom Steitz and Joan Steitz... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog PostPublishedJuly 29, 2014, 6:16 p.m....colleagues built a Molecular Expressions collection including some 3,300 plasmids along with image galleries...specific cellular structures.The Davidson lab even included some of their own reference images that can be... -
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the AAV9-X1.1...have a bunch of other new AAV vectors as well — including next-generation biosensors for GABA, dopamine,... -
Antibodies 101: Affinity Tags
TypeBlog PostPublishedSept. 19, 2023, 1 p.m....tags, often called polyhistidine tags, typically include six consecutive His residues (or more). Histidine... tags There are many different types of tags, including epitope tags (FLAG, Myc, etc.) and fluorescent... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog PostPublishedSept. 12, 2023, 1:15 p.m.... A free, donation-based plasmid analysis tool including editing, annotating, creating maps, and more. ... Expasy: Bioinformatics resource portal which includes both bioinformatics tools and knowledge databases... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m....Acoustic Reporter Genes (ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog PostPublishedMay 18, 2017, 2:30 p.m....biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin... (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium sensor... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....affected. Previous tools to overcome this restraint include an AAV-based sparse labeling system where a limiting...recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable ... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog PostPublishedDec. 14, 2020, 2:15 p.m....receptors that play many roles in signaling processes including smell, taste, sight, inflammation, and neurotransmission... could be measured using the TRUPATH system. Conclusions Although the importance of GPCR pathways is well...