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...checklist as champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already...workflows and keep things moving internally. And remember, a publication isn’t required to share your plasmids...

...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

...insights they glean from a reading session. To remember what happened when drug X was added to cell line...with a specific gene knock out, often you have to remember the experiment, not the paper. To get around reading...

...came to visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run ..., the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family, including...

...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

...the stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly...replication and gene transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead...

...These convert the amino acid tyrosine to betanin, a member of a class of pigments primarily found in plants...skill that takes practice and is difficult for new members of the lab or collaborators unfamiliar with these...

...Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly...cell line model for cancer-specific research. Membrane receptors and ion channels are often exogenously...

...useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies on lysosomal...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

... AAV2 interacts with the packaging cell's cell membrane When packaging adeno-associated viral vectors ...supernatant, the viral particles associate with the cell membrane and are taken into the cell via endocytosis. In...

... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

...overhangs to your insert of interest for Gibson assembly. The steps following primer design and the PCR...to ligate our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation ...

...a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry...SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually...

...make sure to rinse off the surface of the filter membrane with the surrounding solution to ensure excess...excess viral particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating...

...where you want to express multiple transgenes, remember that you’ll need to integrate them all at different...dollars, they will inject the plasmid into fly embryos and pick out flies where your transgene has been...

...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

...findability from multiple angles    We assembled a cross-team team, with members from the Customer Service, Data...

...of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into multi-input...multi-input cell classifiers in one assembly step. Find the miRNA reporter collection at Addgene! Gam ...