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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 241 - 260 of 517 results

  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

  2. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

  3. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

  4. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...the stochasticity of molecular interactions and embedding individual diversity to get from gamete to a visibly...replication and gene transcription during vertebrate embryogenesis. My interests, though, have proven unruly. Instead...

  5. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... your research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica...also showed that R-bodies can rupture E. coli membranes. Read the full post for potential applications...

  6. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...overhangs to your insert of interest for Gibson assembly. The steps following primer design and the PCR...to ligate our cDNA into the recipient plasmid. Remember to insert your DNA in the correct orientation ...

  7. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...useful for monitoring mitophagy. It’s important to remember that because Keima’s fluorescent relies on lysosomal...phosphatidylethanolamine (PE) and recruited to autophagasomal membranes, which leads to its degradation by autophagy. ...

  9. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...These convert the amino acid tyrosine to betanin, a member of a class of pigments primarily found in plants...skill that takes practice and is difficult for new members of the lab or collaborators unfamiliar with these...

  10. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly...cell line model for cancer-specific research. Membrane receptors and ion channels are often exogenously...

  11. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...make sure to rinse off the surface of the filter membrane with the surrounding solution to ensure excess...excess viral particles do not remain adhered to the membrane. Be careful when diluting - if you end up concentrating...

  12. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...a CAAX sequence to bind the mRNA to the plasma membrane; this sequence prevents diffusion of the mCherry...SunTag-based system tethers mRNAs to the plasma membrane, it’s not appropriate for use with mRNAs usually...

  13. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ... is important and how it should change how the members of your audience think or act.   To address that...substitute for feedback from others.  Seek out members of your target audience and ask for their feedback...

  15. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

  17. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

  18. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...that has been validated in multiple assay types. Remember that ChIP uses native proteins (so antibodies ...extra-methodical approach" to the top of your protocols when embarking on your ChIP journey!  References and Resources...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

  20. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...
Showing: 241 - 260 of 517 results