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We narrowed to 677 results for: Eng

Showing: 241 - 260 of 677 results

  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...becoming an ever more ubiquitous tool for genome engineering, and studying its structure can help us understand... it’s the reason guide sequences have a maximum length of 20 bases: nothing longer will fit. Stabilization...structural insight also spurs the next generation of engineered CRISPR enzymes with their own tradeoffs. And ...the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant with PAM Flexibility Cas9 vs...

  3. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   I spent a year hoarding...used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint. #deckthelab...

  4. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on ... @ShihCrystal.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...

  5. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...synthetic biology, DNA assembly methods, and engineering cellulose degradation. 18:21 - 23:26 : Kwabena's...projects in Ghana.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...facilitate most experimental applications in CRISPR engineering. When compared to that collective utility, it...This is only one of many decisions faced by those engaging in CRISPR design, but it is a significant hurdle...limitation, but the tool is fast enough that, if you’re engaged in heavy CRISPR design (I’m talking screens and...

  8. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ....     Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called...efficiency and specificity of multiple wild-type and engineered variants of Cas nucleases. In doing so, they ...created a modular toolkit for Cas-mediated genome engineering in plants. Read the paper in bioRxiv Find...

  9. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ... the Collins' lab at the time) got the idea to engineer bacteriophage (viruses that infect bacteria) for...grow with his help! Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

  10. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    Published
    Feb. 25, 2021, 2:15 p.m.
    ...My son is an engineer and my daughter has a business degree. From the very start of their undergraduate...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...Image from pd|hub.   To begin to tackle these challenges, a group of stakeholders came together with the...

  11. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...exciting developments surrounding CRISPR genome engineering in the past year, we're excited to announce that...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...be removed by re-expressing the transposase. A challenge of using PB to generate iPSCs is that genomic ...the cytoplasm independent of the cell cycle. A challenge of using Sendai is that since it’s replication...clones tested had plasmid integration. Another challenge is that multiple transfection makes it difficult...induced pluripotency: Recent progress and future challenges." Journal of Cellular Physiology 228, no. 2 (...

  13. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... some challenges during my internship, but nothing could have prepared me that those challenges would ...exploring my different interests but also a way of challenging what I am used to. Brandon Solis, marketing...

  14. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE adenine base editor variants using a protein-truncation...activation-induced cytidine deaminase (AID) and an engineered tRNA adenosine deaminase (TadA) to a catalytically...packaging plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable ...

  15. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...co-op) is what has brought me, a second year bioengineering student, to Addgene. I am currently working...interview. I anxiously prepared for the call. The length of this interview was intimidating to me, as I ...comfortable.     Lucie Wilson is a rising junior and bioengineering student at Northwestern University. She is ...

  16. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...cycle of one step. Challenges in the LEGO science video process One major challenge when we started was...will bring people together and make these daily challenges a little easier to overcome. We want to encourage...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...fluorescent protein-based sensor. This sensor was engineered from a GFP variant and a DA receptor such that...PMCID: PMC3083031. Kebabian, John W., and Paul Greengard. "Dopamine-sensitive adenyl cyclase: possible ... Kebabian, John W., Gary L. Petzold, and Paul Greengard. "Dopamine-sensitive adenylate cyclase in caudate...

  18. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and experimental ...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin...

  19. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    .... The resultant JEDI-2P sensors overcome many challenges in 2-photon in-vivo imaging of voltage fluctuations...neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins. HcKCR1 ...work also proposes promising avenues for future engineering to shift the spectra and improve the performance...

  20. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...disciplines or doing different tasks. But these challenges can be exaggerated when communicating across ... help other teams successfully overcome these challenges, too. Schedule regular meetings Scheduling a...
Showing: 241 - 260 of 677 results