We narrowed to 285 results for: Fus

1. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ... fly stocks and other reagents. If you’re ever confused about the best way to study YFG in flies, there...

2. PhD Applications After COVID

   Type

   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...funded? Are there any financial aspects that are confusing? During a traditional campus visit, many students...

3. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   Published

   Sept. 6, 2016, 2:30 p.m.

   ...different applications such as fluorescent protein fusion studies and the generation of mutant cell lines...

4. Google Forums Round Up: First Impressions of NgAgo

   Type

   Blog Post

   Published

   Aug. 4, 2016, 2:30 p.m.

   ...system indicates that it is very tricky to use and fussy to optimize. One reason could be the instability...

5. PITChing MMEJ as an Alternative Route for Gene Editing

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   Blog Post

   Published

   Feb. 23, 2016, 3:30 p.m.

   ...construct is inserted into the PITCh vector via In-Fusion or SLIC cloning. Step 2: Design an insertion locus-specific...

6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ... single point mutation? Do you want to create a fusion with a reporter protein such as GFP? All of these...

7. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ... bioreactor supports high density HEK293 cell perfusion culture for the production of recombinant Erythropoietin...

8. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

   Type

   Blog Post

   Published

   Oct. 4, 2018, 12:44 p.m.

   ...directed binding site. These methods include the fusion of an activation domain such as VP16 to dCas9 or...

9. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...Cas9 expression is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic...

10. Plasmids 101: Secondary Nanobody Toolbox

    Type

    Blog Post

    Published

    Feb. 27, 2018, 2:04 p.m.

    ...sacrifice and precludes genetic engineering of a tag or fusion with a reporter enzyme. For multicolor staining...

11. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...inquiry – purification and characterization of a fusion protein: Histidine tag, malate dehydrogenase, and...

12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...

13. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...also small enough to deliver with AAV. This system fuses catalytically dead dCas13b to the catalytic domain...

14. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...Editing for Programmable A to I Replacement) system fuses Cas13b to RNA deaminase ADAR2 to create a specific...

15. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    .... They don’t simply rely on chance (or random diffusion) to make this happen.  There are several different...

16. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ... containerization, but haven’t tried it or are confused by the concept, don’t worry–you are not alone!...

17. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    Published

    July 25, 2019, 12:03 p.m.

    ...e.g. GFP) can be used in many ways. Often a FP is fused to a protein of interest to elucidate the subcellular...

18. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type

    Blog Post

    Published

    May 2, 2019, 12:41 p.m.

    ...economic importance as harmful weeds. The parasitic fusion is such that the two plants share RNA, proteins...

19. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting a different...

20. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ... Our Stem Cell Collection Transcription Factor Fusions for Enhanced Reprogramming Find plasmids from the...