We narrowed to 317 results for: MUT

1. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ... gRNA is all you need to generate a frameshift mutation that will permanently ablate protein expression...

2. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

3. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...early-onset cataracts, is characterized by point mutations in the Iron Response Element (IRE) RNA motif of...

4. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ... the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 localizes to the ...

5. New Tool for Lineage Tracing: The ClonTracer Library

   Type

   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

6. Plasmids 101: Golden Gate Cloning

   Type

   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

7. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

8. Mouse Modeling, Part 1: Genetically Engineered Mice

   Type

   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

10. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

11. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

14. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...

16. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

17. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

18. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

19. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

20. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...