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We narrowed to 676 results for: Mos;

Showing: 241 - 260 of 676 results

  1. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...requested in the call. Step 2: Develop a research plan Most grant applications restrict the space available ...more advice on writing a grant proposal. "Murder Most Foul: How Not to Kill a Grant Application" by Vid...process? What types of writing feedback do you find most helpful? Have a great grant writing tip? Share it...

  2. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...allowing everything to stay in the correct order. Most Fragmid components are available for request on ...plasmid a name and generate the vector file. Ta-da! Most (180/181) Fragmid components are available in the... be using more than six or seven components — in most cases, that's making two or more plasmids — it may...

  3. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample and...the least-abundant protein and working toward the most abundant. If the abundance of your targets isn’t...maximize protein retention. Following this step, most PVDF membranes recommend re-wetting with methanol...

  4. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working to develop CRISPR-based...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ... sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...“goldilocks antibody.” Antibodies are one of the most commonly used research tools in scientists’ pockets...kept in mind our own lab’s purposes. The Wu lab mostly uses whole-mount IHC techniques on rodent brains...

  6. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...T-cells were transferred back into the infants. Almost all patients have achieved persistent immunological...distinguish AAVs as gene delivery tools: Unlike most naturally occurring viruses used in clinical trials...that are responsible for integration into host chromosomes, further minimizing potential activation of innate...

  7. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...how molecular biology impacts worm locomotion. Our most recent models of C. elegans molecular biology are...been incorporated as a non-profit entity and so far most of their funding has come from kickstarter and fundrazr...everyone knows the internet loves cats! This is mostly a project to get our computer folks at the makerspace...

  8. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...the Myc tag to the world, it has become one of the most commonly used tags in molecular biology. And the...we all really using the same antibody developed almost 40 years ago? Well…kinda. In some cases, antibody...design experiments. It turns out that even with the most popular antibodies, context is key.    References...

  9. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...point during this interrogation process, they will almost invariably say something like “Oh,…I didn’t quite...protein function in the human body?” … and probably most importantly… "how do we know this?” Over the past...represent one aspect of the protein very well, it will almost certainly fail to convey other aspects of the structure...

  10. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...reference sequence. The five contigs with the most total reads are most likely to correspond to the contaminant...containing the suspected contaminating sequence. In most cases, the source of the contamination can be identified...

  11. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...production site to its final destination. As an example, most secreted proteins are produced in the endoplasmid... selected FPs need to be sufficiently separated, most commonly FPs with red and green wavelengths, respectively...microscopes the detection limit is 200 nm. The size of most proteins is below 10 nm and therefore colocalization...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ... be difficult to directly use in bacteria, where most genetic manipulations start. Anthony Forster’s lab...though expressing the chromoproteins from the chromosome could get around this problem. There is no single... “ideal” chromoprotein plasmid that combines the most desirable features: intense color, fast maturation...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...because you will be part of a team in industry with most team members giving oral presentations, you will...to work on later. For instance, AstraZeneca posts most of its positions all at once at the end of February...method of posting varies between companies. Like most jobs, it does help if you know someone who’s already...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...an extra wash step or use a different strain. For most applications, Addgene uses and recommends DH5𝝰!...recombination, but are also endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes ...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...genetic screening experiments”, it is certainly the most robust. In this blog post, we will discuss how CRISPR...libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are provided at a concentration... of next-generation sequencing. Representation - Most libraries contain 3-6 gRNAs per target gene, and...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ... we’ll break down the mechanism of action of the most common antibiotic resistance genes and which applications...bacteria that express the Tn5 element, but thankfully, most competent cells do not express this factor. Zeocin...?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies? Ampicillin Fair...

  17. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...response. At the time, adenoviral vectors were the most commonly used viral vectors for gene delivery, and...limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot... is back, better and safer. It is now one of the most active and exciting areas of science. This is hopefully...

  18. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...the world encountered COVID-19 at different times. Most notably, China was affected by COVID-19 earlier ...placing orders per day across regions, with Europe most impacted and China least impacted. The inferred ... China COVID-19 date range is, they could be the most impacted or least impacted. These results highlight...

  19. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...packaging and envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the...of research impact and safety. Many of Addgene’s most requested plasmids are used with lentiviral expression...

  20. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ....com.   The problem of antibiotic resistance Most antibiotics target one of three essential processes...are easy to see. As discussed in this blog post, most bacteria have poor non-homologous end joining machinery...specificity - rather than targeting a process essential to most bacteria, custom CRISPR antimicrobials can target...
Showing: 241 - 260 of 676 results