We narrowed to 329 results for: NUC

1. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   Blog Post

   Published

   Sept. 18, 2018, 1:33 p.m.

   ... find the ACE reporter plasmid! Martin et al., Nucleic Acids Research 2018 PubMed: PMID 29746667. ...

2. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

   Published

   Oct. 29, 2015, 2:30 p.m.

   ...transferred between cells. At the time, these extranuclear agents of heredity were thought of as everything...

3. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   Blog Post

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   June 4, 2019, 1:02 p.m.

   ...use the serotonin receptor to study how single nucleotide polymorphisms present in the human population...

4. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

   Published

   Sept. 19, 2017, 1:20 p.m.

   ...surface, in the cytosol, in mitochondria, or in cell nuclei. The two photosensitizers had similar rates of ...

5. With Our New Viral Service, We're Taking CRISPR Further!

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   Blog Post

   Published

   Dec. 6, 2016, 3:30 p.m.

   ...it unuseable. With the large size of the SpCas9 nuclease (~4.2 kb) this is a particularly prominent problem...

6. The Importance of High Titer for AAV Transductions

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   Blog Post

   Published

   March 1, 2018, 2:11 p.m.

   ...through each part of the cell on their way to the nucleus where their gene cargoes can be expressed. The...

7. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   Blog Post

   Published

   Dec. 20, 2018, 1:15 p.m.

   ...intended parents is “rehoused” in a separate, enucleated zygote with healthy mitochondria, with the intent...

8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   Blog Post

   Published

   Feb. 13, 2024, 2:15 p.m.

   ...assays with immune cell lines or peripheral blood mononuclear cells (PBMC) in 3D models of the human lymphatic...

9. Antibodies 101: Monoclonal Antibodies

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   Blog Post

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   June 29, 2021, 1:15 p.m.

   ...antibodies. Genetic drift refers to changes in the nucleic acid sequence of the antibody-encoding genes over...

10. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post

    Published

    Feb. 11, 2021, 2:15 p.m.

    ...activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this platform accurately...

11. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    Blog Post

    Published

    June 25, 2019, 12:54 p.m.

    ...Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the...

12. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...differentiation. Furthermore, TALEN and ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have...

13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...PubMed PMID: 27411589. 2. Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in...

14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post

    Published

    Sept. 15, 2020, 1:15 p.m.

    ...Sanger sequencing determines the precise order of nucleotides within a DNA molecule, in this case a plasmid...

15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...DSB is repaired by NHEJ which depending on the nucleotides inserted or deleted, can result in an in-frame...

16. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ... to perform unique editing tasks, classic Cas9 nuclease, which introduces double stranded DNA breaks, ...

17. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    Published

    Jan. 25, 2024, 2:15 p.m.

    ...the following information for a Cas enzyme: Nuclease properties (CasID): Cis/trans activity, PAM requirement...

18. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    Published

    July 25, 2023, 1 p.m.

    ...proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging,...

19. Viral Vectors 101: Viral Applications

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    Blog Post

    Published

    April 13, 2023, 1:15 p.m.

    ...the delivery mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several...

20. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    Published

    Feb. 24, 2015, 7:50 p.m.

    ... and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating...