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We narrowed to 451 results for: PAN

Showing: 241 - 260 of 451 results

  1. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  2. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...than ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple ...combat sexual harassment in academia or a large company who decides how they are going pursue regulatory...

  3. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...therapies can be developed and applied. The current pandemic serves as a striking example of the applicability...opportunities The world of science is vast and ever-expanding. While it can be tempting to try to plan out a...

  4. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus ...

  5. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...CRISPR CRISPR-Cas can harness the power of MoClo to expand both targetability and effectiveness. Perhaps the...tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other lentivirus...

  6. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...gRNA oligos, preferably as an oligo pool. Some companies that synthesize oligos would even accept the Output_Short...through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR screen...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...detect proteins is referred to as staining. This panel of antibodies is used to both identify the cell ...flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry is the ability to...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...preferences, which they defined thoroughly. Plus, they expanded CGBE target scope by creating BE variants with...CGBEs. They found that C → G editing was often accompanied by small deletions in their target loci (between...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020...ja512664v  Jain PK, Ramanan V, Schepers AG, Dalvie NS, Panda A, Fleming HE, Bhatia SN (2016) Development of Light-Activated...

  10. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...are cases in which it is an appropriate choice (Panina, 2018). Look for studies specifically aimed at ...real-time RT-PCR. J. Immunol. Methods 283:291–306.Panina, Y., Germond, A., Masui, S. et al. Validation of...

  11. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...knockout screens. These vectors and sgRNA libraries expand upon the CRISPR family of plasmids by offering ...protein function and signaling networks continues to expand. The labs of Jesse Rinehart and Dieter Söll at ...

  12. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ...That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a...

  13. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow orchestration...

  14. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...well as alternatives to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com...

  16. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...edition of our CRISPR 101 eBook is now available in Spanish!  This translation was gifted to Addgene by Daniel...

  17. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX...

  18. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...we've partnered with distributors in China, Korea, Japan, India, and Brazil.  We've also created a robust...

  19. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling Ashlyn Lemmen, who...
Showing: 241 - 260 of 451 results