We narrowed to 290 results for: Pol;

1. Plasmids 101: Degron Tags

   Type

   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein...

2. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

3. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   Published

   May 18, 2021, 1:15 p.m.

   ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

6. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

8. Uncovering Ancestral Bias in CRISPR Research

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   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

9. An Introduction to Adenovirus

   Type

   Blog Post

   Published

   Oct. 3, 2023, 1:15 p.m.

   ...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

10. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    Published

    Jan. 16, 2020, 2:18 p.m.

    ...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use...

12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    Published

    Oct. 6, 2015, 2:30 p.m.

    ...has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube...

13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    Published

    Aug. 13, 2019, 12:58 p.m.

    ..., including investigations into environmental pollutants and health, embryo growth, brain function, and...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...

16. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...

17. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    Published

    April 19, 2018, 1:25 p.m.

    ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    Published

    Sept. 16, 2020, 1 p.m.

    ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

19. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...

20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...