We narrowed to 347 results for: SAM-2

...majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry requestors... over.” And we sent the Addgenies home.  At the same time, as Addgene started dealing with this new reality...and keep new projects on track to succeed at the same time. I began approaching science funding organizations...

...melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle... 2015). There, it divides into two cells. At the same time, it sucks out its contents (hence the name ...

...of different cell types within a sample to expected labeling (Figure 2). The panel of antibodies used in...then labeled with the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal... recognize different epitopes of the same protein, show the same pattern of labeling, this is supportive...detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly map...determining the selectivity of antibodies for other sample types and for non-surface (intracellular) antigens...protocol used and the abundance of the epitope in the sample, relative to cross-reactive antigens – that is,...approaches used will be different depending on the sample and target type. To start the validation process...

...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...endogenous WT levels of the same protein when both are expressed in the same system. Tagging can also aid...EQKLISEEDL) derived from the protooncogene of the same name. The tag has a net negative charge with 4 negatively...

...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...accommodate adeno-associated viral vector (AAV) samples and created a simplified process, termed viral ...parallel, there is a very real risk of mixing up samples, serotypes, or both. Unfortunately, the standard... as [+] and [-] strands that are packaged at the same frequency, we reasoned that, following DNA extraction...

...retransduce a model organism such as mice, e.g. first at 2 months, then at 4 months? Or do antibodies become ... A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...of co-delivering different AAV particles into the same cells in vivo? A: This will be highly dependent ...

...create a recombinant antibody.     Figure 2: Changes to the heavy chain or light chain genes within...Monoclonal” refers to antibodies that are all of the same isotype and specificity. In other words, monoclonal...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...antibodies that recognize many different epitopes on the same protein - are more likely to have off-target effects...produces high-affinity/high-avidity antibodies. The same hybridoma is then used to produce all lots of the...

...account when simulating a digest as depicted in Lane 2. Controlling methylation Finally, you can control... experiment? The answer to both questions is the same--methylation! Read on to learn about how DNA methylation...companion endonucleases that recognizes and cleave the same DNA sequence if not methylated (if it comes from...Although not all prokaryotic DNA is methylated to the same level, the potential for methylation should be considered...any) of those sites will be methylated. Using the same example plasmid as above (which has the XbaI site...

...Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338-6  Grimm, J. B., Muthusamy, A. K., Liang... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...cytoplasmic background signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes...

... and transfer 100 µl of glycerol stock to each of 2 96-well microplates (identical copies, see image above... that consistently receives around 200 new DNA samples every week. Here we will provide an inside look...own plasmids. Staying organized With hundreds of samples coming through our doors, the first step to ensuring...our deposit process, and then we carefully track samples through our lab using barcoded tubes and plates...process. Always sequence verify Once the physical sample and its associated information are at Addgene, ... will catch any errors made during the cloning, sample handling, or sequence assembly steps. All of our...This means that we are sequencing from the exact same stock that we store and distribute from. Of course...

...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids...point of reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and ...libraries, but any analysis script should return the same output: a spreadsheet of read counts. You can use...

... adapted to a competitive format. Figure 2: Overview of using Competitive ELISA to quantitatively...wells with an experimental sample allows for detecting one protein from the sample. A direct ELISA has many...require scientists to detect proteins in their samples. One technique that relies heavily on antibodies...principle of an ELISA is that any antigen within a sample adheres to the well (either directly or by binding...antibodies coating the wells to capture antigen from a sample, whereas antigen is bound directly to the wells...protein you are interested in detecting in your sample. When an ELISA kit is ordered, the wells are typically...already coated with the antibody. Then, you add your sample to the wells, and the antibodies bind to your protein...

..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells...two loxP sites, which can be located either on the same or on separate pieces of DNA. Both 13bp repeat sequences... sites: Inversion: If the loxP sites are on the same DNA strand and are in opposite orientations, recombination... reversed.   Deletion: If the sites face in the same direction, the sequence between the loxP sites is...sites undergo recombination with other sites of the same type, but are not cross-compatible. Using different...

...(S) labeling of the cell population. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling...allows for the labeling of a subset of cells with the same genetic makeup, overcomes all of these labor-intensive...

... master's degree prepared them for their career.  2. Are you looking for career advancement and growth...handful of assays over and over. Everyday was the same and I was bored. I wanted more variety and more ...

...closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique Detection...PMID: 23792628. PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet...investigator can be relatively certain that their samples don’t have off-target mutations if they remain ...

...PMID: 30356044. PubMed Central PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep... codons they use; even if two species encode the same protein, their gene sequence may have slight or ...

...two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a ...format. Figure 6: Add sequence, maps, and files. Step 2: Gene and Insert *Note: This page will not appear ... library is a set of plasmids all built with the same backbone and only differing in the gene/insert. ... Pro Tip! If you have multiple plasmids with the same gene or vector backbone, you can copy some information...

...perceived climate change risks. Nature Climate Change, 2(10), 732-735. doi:10.1038/nclimate1547 Kunda, Z. (...fitting that I’ve ended up (again) dispelling the same myths and misconceptions fifteen years later. What..., peer-reviewed study. The second group read the same abstract but with the names reversed, meaning that...

...small, helpful changes are endless.    Fig. 2: Blugene moves reagents from left to right as they...running it unchanged over and over again, and the same goes for your workflow. Next time you make a mistake... to match your plate layout can help you track samples as you add them. Moving your reagents from your...