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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 498 results for: SHA

Showing: 241 - 260 of 498 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...designing the best donor DNA, and avoiding common mishaps in this class of genome edit. Knock-in mutations...2020). 10.1016/j.jbiotec.2019.11.010 Wright, D. W., Shah, S. S., Heyer, W. D. Homologous recombination and...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore concealed at the center of...Gal Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...visualize numerous molecules or structures at once (Shaner, 2007). In contrast to epitope tags, FPs are bigger...2009.12 (2009): pdb-top63. PubMed PMID: 20150100. 4. Shaner, Nathan C., George H. Patterson, and Michael W....

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...frequency in a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the...Genet 12:683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure...

  8. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...make a career in academia, you are likely in good shape, as your own adviser will probably have a lot of...webpages. Importantly, find a good mentor that can share relevant experience and resources with you to ease...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...have a shape, like a box, ellipse, or circle, drawn around clusters to define populations. This shape is ...

  10. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and write down where that...that one drug is stored or how to access the shared server. The blue box labeled “A5” on the second rack...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...at the limited number of hits. So, here, I will share my process with you. But before I do that, let me...formatting guidelines, etc.) you have some criteria to shape the document. Get and use a reference management...

  12. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...knowledge to a variety of problems. In this post, I’ll share what I’ve learned as a healthcare consultant and...as marketing drugs to physicians and patients, shaping discussions with insurance companies about the ...

  13. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...you are making your hypothesis stronger. When you share data at a conference poster session, you don’t necessarily...  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization...

  14. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...tissue morphogenesis, organ development, and the shaping of the extracellular matrix. During immune responses...cellular processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...

  15. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...the fine points of visual aids. Here’s a great slideshare with more details on visuals in science presentations...down it is a great segue to talk about how the sharing model works. See if you can elicit helpful comments...

  17. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ... E. coli strains E. coli are gram-negative, rod shaped bacteria that were named after Dr. Theodor Escherich...strain is a bit more convoluted due to researchers sharing and renaming samples throughout history. It was...

  18. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak,...limitations of existing backbones, we're excited to share our very own set of empty, HA-, and EGFP-expressing...

  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...doi.org/10.1038/s41467-023-39554-7 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. ...j.neuron.2016.09.021 Walters, R. W., Yi, S. M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. ...

  20. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...binding pocket of an engineered receptor that change shape with light exposure to activate them. PORTLs use... Chibukhchyan, A., Ramakrishnan, C., Inoue, M., Shane, J. C., McKnight, D. J., Yoshizawa, S., Kato, H....
Showing: 241 - 260 of 498 results