We narrowed to 351 results for: SUA

1. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

   Type

   Blog Post

   Published

   May 5, 2015, 3:54 p.m.

   ...some sort of model (cartoons are appreciated) to visually sum up all of the conclusions. In any case, the...

2. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...how the output changes as a function of input. Usually that function is in the shape of an S; there is...

3. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ...this is a style that will help you. A good coach usually will be a clear communicator and make direct suggestions...

4. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...these resources allow QC scientists to easily visualize and identify sequence differences between our ...

5. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...sites. Knock-in experiments are important for visualizing ncRNA localization and function, but directly...

6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...Vogelstein showed, for the first time, there were unusual patterns of DNA methylation detected in a human...

7. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...non-homologous endjoining (NHEJ). Cas9-mediated NHEJ usually destroys the PAM site due its proximity to the ...

8. Plasmids 101: Terminators and PolyA signals

   Type

   Blog Post

   Published

   March 31, 2016, 2:30 p.m.

   ... transcript. Rho-dependent terminators are not usually employed in plasmid-based expression systems, so...

9. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...the Addgene Blog Learn about techniques for visualizing protein turnover Learn about plasmids for in ...

10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ... one’s figures, or at least the majority of the visual data. This central box is then flanked by two equally-sized...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...spreadsheet to complete Step 1. Steps 2 and 3 are usually very easy — our tech transfer team will communicate...

12. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...would like to monitor? Neurons, for example, have unusually fast calcium dynamics, whereas non-neuronal systems...

13. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus ...

14. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ... lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain...

15. Gaining Leadership Skills Volunteering at a Professional Organization

    Type

    Blog Post

    Published

    Aug. 6, 2015, 2:30 p.m.

    ...Volunteering on these committees was real work; meetings usually resulted in actionable tasks and I made a point...

16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...difficult people: Hostile-aggressives, who are usually antagonistic and impolite, Complainers, who spend...

17. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...infection rates in your experiment. Figure 2: Visual representation of common AAV serotypes used for...

18. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...production when roughly 60–70% of cells have died, usually after four to five days. This means that viral ...

19. PCR Applications for Copy Number Variation Assays

    Type

    Blog Post

    Published

    April 26, 2022, 1:15 p.m.

    ...sequence present in the same amount in all samples (usually to a ubiquitously expressed, one-copy ‘housekeeping...

20. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...temperature cycling. The final PCR product is often visualized on a diagnostic agarose gel to confirm product...