We narrowed to 520 results for: Set
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  TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....that is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...cell types of interest. By correlating your data sets, you can increase confidence that the labeling observed...available. Various proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g...
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  Advice on Career Paths and the Green Card Process for International Researchers and EntrepreneursTypeBlog PostPublishedDec. 20, 2017, 2:33 p.m....Buy American, Hire American" Executive Order which sets broad policy intentions directing federal agencies...ensure that each candidate meets the requirements set forth by USCIS before engaging with clients. To learn...
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  Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable ProteinsTypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...
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  Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity ToolsTypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...
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  Summer SciComm: PreprintsTypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....submitting patent or grant applications; to informal settings like lab meetings, social media, coffee chats ...
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  Antibodies 101: Polyclonal AntibodiesTypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....compare to other antibody options out there, you’re set up for a successful next experiment! References...
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  Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....). A control RFP expression plasmid. With this set up, all successfully transfected cells will express...
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  Expanding the Targeting Scope and Editing Efficiency of Adenine Base EditorsTypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions. The need for speed: base...
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  Viral Vectors 101: BiosensorsTypeBlog PostPublishedJuly 6, 2023, 1 p.m....the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some...
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  Viral Vectors 101: Types of virusesTypeBlog PostPublishedJune 6, 2023, 1:15 p.m....therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools...
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  Viral Vectors 101: Viruses as Biological ToolsTypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing...
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  Stabilized Bacterial Promoters: Constant Gene Expression at any Copy NumberTypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome...
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  Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and in live zebrafish. For... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...
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  Choosing the B(right)est Fluorescent Protein: Aggregation TendencyTypeBlog PostPublishedJune 15, 2017, 2:30 p.m....tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions...
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  Visualizing Protein Turnover In SituTypeBlog PostPublishedJan. 16, 2018, 3:20 p.m.... not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in...and specific antibody and the proper microscope setup we can observe the whole population of one particular...
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  Developing a cold tolerant E. coli using specialized chaperonesTypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating...
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  New Tool for Lineage Tracing: The ClonTracer LibraryTypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...
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  CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher VoigtTypeBlog PostPublishedApril 22, 2015, 2:06 p.m....for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...
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  Transferable Skills Guide: Cross-team CommunicationTypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....solution can easily be implemented in an academic lab setting, where student and postdoc turnover happens regularly...
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  A Guide to Designing a Scientific Poster: Content, Layout, and MoreTypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....when thinking about crafting your own poster. What sets a scientific poster apart? First, a good poster ...