We narrowed to 511 results for: Set
-
TypeBlog PostPublishedDec. 6, 2018, 1:34 p.m....Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...
-
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....). A control RFP expression plasmid. With this set up, all successfully transfected cells will express... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions. The need for speed: base... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog PostPublishedJan. 28, 2020, 2:35 p.m.... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog PostPublishedSept. 19, 2017, 1:20 p.m....with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and in live zebrafish. For... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....submitting patent or grant applications; to informal settings like lab meetings, Twitter, coffee chats with ... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....compare to other antibody options out there, you’re set up for a successful next experiment! References... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog PostPublishedFeb. 2, 2018, 3:20 p.m....current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....connections This is a site for meeting people. Use the settings to let people see who you are when you look at... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....its surroundings and being shaped by them. Another set of highly influential microenvironment cells are ... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....deposit to Addgene. We are also lauded by another set of stakeholders - the tech transfer community. In... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog PostPublishedSept. 8, 2020, 2 p.m....“We hope to use CRISPR libraries from Addgene to set up assays to see what molecules or proteins are involved... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...