...tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions...

...). A control RFP expression plasmid.  With this set up, all successfully transfected cells will express...

...applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions.  The need for speed: base...

... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating...

...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...

...the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some...

...therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools...

...stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing...

...compare to other antibody options out there, you’re set up for a successful next experiment!     References...

...submitting patent or grant applications; to informal settings like lab meetings, Twitter, coffee chats with ...

...interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome...

...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...

...for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

...current position at Fred Hutch because her skill set wasn’t matching up with the local job postings. “...

...Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces...few such methods exist and they only work for a subset of kinases. The Problem:  There is no common method...

... et al, 2008) occasionally and certainly after a set-up has been changed (for instance after replacing...

...gene. In this manner, we have created an initial set of 10 plasmids representing 10 human genes and will...Last updated on Oct 14, 2020 by Seth Kasowitz. This post was contributed by the gene editing team at ...

...blast from the past: Cofounder and CSO Melina Fan setting up Addgene's very first in-house NGS run.   ...approvals. In 2013 we were thrilled to receive the Massachusetts Nonprofit Network Excellence Award for our electronic...

...your blot with multiple antibodies. You’ll want to set up your blot with a polyvinylidene difluoride (PVDF...