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Showing: 241 - 260 of 482 results

  1. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...depend on the host cell’s initiation proteins to start their own replication. In general, these plasmids...binding of the low-affinity versions is required to start the replication process. When enough copies of ATP-DnaA...

  2. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...screening experiments as well). Therefore, if you’ve started down the CRISPR path, you’ve probably got a gene... them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing in mice, they...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...consultants advise pharmaceutical companies, biotech start-ups, health insurance companies, and governments..., investment and equity research firms, biotech start-ups, venture capital firms, and more. Essentially...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...homology to the crRNA or presence of a PFS. This is in stark contrast to Cas9, which requires that each DNA target... we know about its structure and function? For starters, Cas13a can be used to bind and cleave target ...

  5. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...means exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic... gene Although this list is a great place to start, the tables above do not delve into the tissue or...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...Notebook. Implementing an electronic lab notebook starts with defining your lab’s needs The values of ELNs...lab notebooks into 9 questions that will help you start and define what exactly your lab needs from an ELN...

  7. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ... a mutation introduced by CRISPR. The sequence started clean and then, about 100 bps into the run, it ...CRISPR for mouse genome engineering From the very start, generating mouse models using CRISPR is easier ...

  9. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... annually advertise for Group Leader positions starting in August, the year before the position would ...PFs are in committed relationships and/or have started a family. Those people are your support network...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... is all it will bind to - particularly when you start using complex samples such as whole cell lysate ...be aware of any possible alternative translation start sites or alternative splicing that could lead to...

  11. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...piggyBac is biased to integrate at transcription start sites, CpG islands, and DNaseI hypersensitivity ...Tol2 also prefers to integrate at transcription start sites, CpG islands, and DNaseI hypersensitivity ...

  12. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...breaks Prediction algorithms: A good place to start for biased validation At the moment, many software...sequences of the genome and sgRNA. This is a good starting point for most researchers as it provides a list...

  14. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...popular whole cell therapeutics because they can help start a sequence of immune reactions that aid in the fight...article, cancers differ in practically every facet starting from manifestation to their specific interactions...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...transversion editor The story of base transversion editors starts with researchers in the Keith Joung lab, who were...as AP sites. To explore this further, the team started engineering a CBE to be worse at its original ...

  16. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...specified by the Cas protein These elements were the starting point towards the development of the first cytosine...DNA before the edit is made. Using ABE7.10 as a starting point, Gaudelli evolved the base editor into 40...

  17. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...methods and buffers to choose from. If you are starting from tissue, you’ll likely want to use a mechanical... to be read fairly soon after the reaction has started. Types of chemiluminescence enzymes The two most...

  18. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...applications in your experiment...but not sure where to start or what your options are? Addgene's latest animation...

  20. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...winning submissions and deliver the prizes at the start of the New Year. Will your lab be a lucky winner...
Showing: 241 - 260 of 482 results