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We narrowed to 542 results for: TIL

Showing: 241 - 260 of 542 results

  1. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...transferred into the plant. In the laboratory we can utilize this natural process by replacing the genes on ...genes and speeding up research. Certainly, there is still more to transient leaf transformations, but we hope...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...elements that are versatile and adaptable. Variation in ori structure allows plasmids to utilize diverse host...

  3. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... afford to wait, you can hold off on genotyping until the pups are 2-4 weeks old and use the ear tag method...

  4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ... genome. Without a working integrase, IDLVs can still deliver their genetic payload to the cell but cannot...avoid the risk of insertional mutagenesis while still allowing for stable expression of the transgene....

  5. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, ...

  6. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...ordering food - everything is covered.   So why are we still printing and gluing pieces of paper in notebooks... needs The familiar paper-based lab notebook is still a standard approach to record-keeping in many labs...

  10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal...

  11. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...

  14. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ... models, and new Cre-lox based technologies are still being developed today. In this post, I will highlight...labeling system - a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...cells. Flexible tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...

  20. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...
Showing: 241 - 260 of 542 results