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  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, ...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ... genome. Without a working integrase, IDLVs can still deliver their genetic payload to the cell but cannot...avoid the risk of insertional mutagenesis while still allowing for stable expression of the transgene....

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  4. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...ordering food - everything is covered.   So why are we still printing and gluing pieces of paper in notebooks... needs The familiar paper-based lab notebook is still a standard approach to record-keeping in many labs...

  5. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ... models, and new Cre-lox based technologies are still being developed today. In this post, I will highlight...labeling system - a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids...

  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

  11. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

  13. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...and therefore it is important that the digest go until completion. The time required for complete digestion...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

  14. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...research in a lab in California. I felt then, and still feel now, that it is important to take advantage...often don’t tell you what company a job is with until just before the interview). This wasn’t exactly ...

  16. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ... directly injecting DNA into the pronuclei of fertilized eggs, which leads to random incorporation of ...genome after injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA ...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...’ve found that the advice in these resources is still valuable for anyone in the lab though, including...but didn’t realize I was interested in microbes until the end of my second year! Conclusion and other...

  18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...and circularization of dsDNA (6). Less commonly utilized in vitro, Taq DNA ligase will ligate only nicks...Despite this higher fidelity, Taq DNA Ligase can still detectably ligate many T:G, T:T, and A:C mismatches...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...Twitter.   References Arribere JA, Bell RT, Fu BXH, Artiles KL, Hartman PS, Fire AZ (2014) Efficient Marker-Free...Schmidt H, Krause MW, Seydoux G (2014) Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology...
Showing: 241 - 260 of 532 results