We narrowed to 546 results for: TIL;

...the adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at ...rates ranging from 4.7% to 30% at tested sites. Still, it opened a new avenue for base editor development...

...that could produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed... doxycycline prior to FACS, gating for the top quartile (or higher) of BFP and GFP producing cells. After...

... however, is fight smarter, not harder. We can utilize tactics like rotating antibiotic treatments, so...antibiotic is minimized, while the infection is still effectively treated. We can prescribe antibiotics...

... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such... afford to wait, you can hold off on genotyping until the pups are 2-4 weeks old and use the ear tag method...

...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

...ordering food - everything is covered.   So why are we still printing and gluing pieces of paper in notebooks... needs The familiar paper-based lab notebook is still a standard approach to record-keeping in many labs...

...has no strong affinity for other proteins, it may still block the function or activity of the tagged protein...plasmid-based system to assess whether the chimera is still functional. Final words on fluorescent protein fusions...

...respectively, cells will fluoresce either red or orange until recombination occurs. Following the development...ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be induced in ...

...are packaged, the physical size of the genomes is still ~4.7kb. But despite this, larger functional transgene...Gibbs D, Kim SR, Maguire A, Rex TS, Di Vicino U, Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio...

...GFP-like probes by supplying a single gene to cells. Still in bacteria, which do not produce BV, an additional...fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, ...

... genome. Without a working integrase, IDLVs can still deliver their genetic payload to the cell but cannot...avoid the risk of insertional mutagenesis while still allowing for stable expression of the transgene....

...engineering world by storm owing to its ease of use and utility in a wide variety of organisms.  While much of ...X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal...

...exist for this, the most common of which is the utilization of phosphomimetic residues. The phosphorylated...tRNA that recognizes a specific ‘open’ codon and utilizes this triplet to incorporate phosphoserine A Sep-tRNA...

... in the middle of your insert. Gateway cloning utilizes λ integrase to catalyze directional cloning of... Brook Pyhtila...

... models, and new Cre-lox based technologies are still being developed today. In this post, I will highlight...labeling system - a perfect example of the continued utility of Cre-lox. Check out our previous blog post, Plasmids...

...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

...with a light-pulse at one wavelength and stay on until a second pulse of light, with a different wavelength... number of strategies have been developed for utilizing light to control GPCR activation and downstream...

...how a virus enters the cell. Different serotypes utilize a number of different receptors, co-receptors, ...cells express your gene. Off-target expression can still occur (Fig. 3) - for example, some neurons can transiently...

...cells. Flexible tagging: Choose untagged or add a versatile N- or C-terminal HA tag. Selectable and stable... embryonic development and cancer. But there is still much to learn about their myriad functions and therapeutic...

...diverse biological entities on our planet, but we still know collectively little about how they infect and...be understood. Therefore, in addition to their utility in the health sector, phages represent a reservoir...