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We narrowed to 886 results for: URE

Showing: 241 - 260 of 886 results
  1. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...papers or “hot topics,” so be sure to be well-read on the recent literature in your field. Take your time...about to present your first scientific poster, but unsure how best to prepare. Maybe you’re a presentation...attending, your preparation and day-of game plan will ensure you have the most beneficial scientific conference.... For example, when thinking about discussing a figure, jot down the question you were trying to answer... to say and provide your audience with a clear picture of your work. Practice, practice, practice: Rehearse.... Finding your poster board well ahead of time ensures you aren’t scrambling to tack up your poster seconds... the poster, as opposed to engaging with you? Be sure to use your poster as a visual aid, not a crutch...
  2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...for delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane... addition to the use of this technique in cell culture, it has also been applied to genome editing of ..., such as lipid-mediated transfection (B in the figure above), are in the early stages of development....Cas9 proteins harboring receptor ligands (C in the figure above), which result in the internalization of ...transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods...equipment, such as a gene gun. Depending on the nature of the experiment, it is up to the researcher to...protein-based genome editing in vitro and in vivo." Nature biotechnology 33.1 (2015): 73. PubMed PMID: 25357182...
  3. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... to learn more about nanobodies and how their structure and function compare to IgG antibodies, as well...llamas, alpacas and other camelids. In terms of structure, HCabs are like a pared down version of a standard... nanobody, HCab, and traditional IgG antibody structures. Functional comparison Strong but Large: Advantages...big enough that multiple labels (green circles in figure 2 below) can be attached to each antibody. But...standard IgG secondary antibody (Pleiner et al figures 4A-C). And since they have limited cross-reactivity...colocalization labeling of up to 3 targets (Pleiner et al figure 4D). Unlike large secondary antibodies, small nanobodies...eliminating the secondary antibody incubation. See figure 4A below for comparison of this one-step staining...
  4. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...the ability to easily cure cells of the plasmid after integration. Figure 2: (A) Streamlined VchINT...species not previously cultured or isolated (Rubin et al., 2020).  Figure 3: Multiplexed insertions...transposon end sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon...produce off-target DNA insertions and intermediate structures known as cointegrates (Vo et al., 2020; Strecker...achieved with VchINT, at two different incubation temperatures. Data adapted from Vo et al., 2020. In E...targeting by a transposon-encoded CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019...–Cas systems direct RNA-guided DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019...
  5. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...science communications: podcasts, blogging, videos, lectures, exhibits, social media… how do you choose? Which...Them All, consider the following four questions to figure out which method(s) work best for you, your audience...-speed internet. Before you begin any work, make sure you know if your audience will be able to access...never know what's going to work. After all, a stick-figure internet comic about math probably didn't sound...needed. And since we live in the digital age, make sure to research any software you may need: cost or freeware...accessible or most appealing to you. And if you’re not sure what all the possibilities are, check out our table... useful for data-focused stories. In-person lectures, presentations, etc… Low (except for space) Real-time...
  6. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...plasmid miniprep Starting material: 2 mL bacterial culture Product: plasmid DNA This kit-free plasmid miniprep...extraction kit. First, you lyse the bacteria and denature the DNA and proteins in solution. The pH is then...glass syringe filters Starting material: bacterial cultures or agarose gel slices Product: plasmids or DNA...hydrophobic glass filter. Each glass filter can capture up to 150 ug of DNA, and filters can be stacked...together in series to increase binding capacity. Figure 1: Purify DNA using glass filters. Image: Kim and...filters you use depending on the size of bacterial culture you’re working with. Overall, the glass syringe...and without degradation during the extraction procedure. Additionally, purified DNA was free of agarose...
  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...proportion of virulent S. aureus from 50% to 11% in just 24 hours.   Figure 1: Single-phage strategies...targeting sequences found only in certain bacteria (Figure 1). Citorik et al. targeted beta-lactam and quinolone...al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. Two rounds ...system to resensitize antibiotic-resistant bacteria (Figure 2). The first phage introduced is lysogenic, integrating...resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize bacteria...CRISPR, we’re turning the tables on the bacteria. Future research will show if this new strategy can help...using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID...
  8. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating the bacteria...largely absent in adult organisms, where tissues structures are more stable and local movements predominate...than the static microscopy images of chemically coloured lymphoid organs did in the past. How do researchers...electronic transition in a fluorescent molecule (see figure to the right). The excitation generated by these... a chosen focal volume and thus the microscope captures only fluorescence coming from this volume. The...environments. These studies provide quantitative measurements of cellular motility, interactions, and response...peripheral interfollicular region of lymph nodes." Nature immunology 9.2 (2008): 155-165. PubMed PMID: 18193049...
  9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... at the nucleotide level but share an overall structure of 13 base pair palindromic repeats separated ...efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart...Visualizing single neurons in the living brain   Figure 1: Plasmid mix to label neuronal morphology (eYFP...transfection efficiency. However, if the cellular structure of a neuron and its synapse should be visualized...Combing FLP and Cre for additional control   Figure 2: Expression of a morphological marker (eYFP) ...FLP Recombinase Evolved by Cycling Mutagenesis.” Nature biotechnology 16(7): 657–62. PubMed PMID: 9661200...
  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org/10.1038/nature14299  Senís E, Fatouros...bacteriophage and plasmid DNA. Nature 468:67–71 . https://doi.org/10.1038/nature09523  Ibraheim R, Song C-Q, ...SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure of Cas9 in Complex with Guide RNA ... pool of shorter orthologs, only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed cleavage...a distinct family of RNA-guided genome editors. Nature 566:218–223 . https://doi.org/10.1038/s41586-019...
  11. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    Published
    Feb. 18, 2020, 2:15 p.m.
    ...minutes to point me in the right direction for my future work. She graciously took me for coffee and spent...conference for any reason was a Singleton. Figure 1: The Binning Singletons logo. Image: James et...that binning Singletons together would provide a structure and support network for people attending large...improvement. We will make a greater effort in the future to match people within similar fields (ASM Microbe...that all participants would benefit from more structure with regard to the Mentor-Singleton relationship...addressing. Clear expectations have been established for future participants regarding the activities and time ...scientific community can improve the diversity and culture of science. We want Binning Singletons to be another...
  12. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...requires stringent biosafety measures, thereby increasing the costs of the procedure. Episomal reprogramming...fibroblasts, which require a more invasive skin biopsy procedure. This becomes a major benefit when recruiting ...reprogramming success [1]. Fibroblasts need to be cultured and expanded in vitro for several passages to ...collection [2]. PBMCs display unique epigenetic signatures that are closer to hiPSCs than age-matched fibroblasts....03%) [3, 9]. In 2011, Chou et al. generated a mixture of two vectors that encoded six factors - OCT3/... system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding the...cells with unique epigenetic and gene expression signatures. Cell Res, 2011. 21(3): p. 518-29. PubMed PMID...
  13. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...about citing: I wasn’t sure if I should cite reviews or the primary literature or both. I ended up citing...lists and pictures are the most useful parts of reviews. These could be in the form of figures/schematics... to write, to read efficiently, to search the literature, and to synthesize a large volume of information...well-researched field, you may need to get specific to make sure your article doesn’t turn into a textbook. Get the...active/well-known researchers in the field. I made sure to find information from genome-wide studies, as...appreciate the discussion sections of the primary literature as a resource that both summarized results and...Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving...
  14. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...recombination sites (attL-left- and attR-right-, Figure 1). Under certain conditions, the attL and attR... regeneration of attP and attB sites.   Figure 1: Lambda phage integration and excision reactions...gene is excised from the donor vector.   Figure 2: The Gateway system adopts phage integration ... program to design Gateway plasmids.    Figure 3: Method A to create an entry clone: recombination...into an attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning...) of an attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction...clone to create the expression clone.    Figure 6: Generating an expression clone. The reaction...
  15. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold... guidance issued by the Department of Labor. At Future of Research, our volunteers have been helping to...their postdoctoral salary scales to the NRSA scale (Figure 21, National Postdoctoral Association Institutional...legal justification of this have not been received. Future actions for junior scientists and for academia ...many postdocs will immediately lose jobs; that tenured professors/mid-career scientists/junior faculty...biomedical enterprise in the U.S. will decrease. At Future of Research, we think this is a good thing. Reliance...McDowell is currently the Executive Director of Future of Research. He is interested in advocating for...
  16. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...automatically using motion capture software such as EthoVision. Consult the literature to find all the applications... is an approximation of reward or the sense of pleasure that the animal perceives through taking a drug...may affect the animal; stimulation could induce seizures or some other unwanted phenomenon or it could ... elegans [10]. If used properly, optogenetics is sure to teach us much in all of these species. Happy ... nucleus accumbens facilitates reward seeking." Nature 475.7356 (2011): 377-380. Pubmed PMID: 21716290...direct comparative analysis of microbial opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al...."An optogenetic toolbox designed for primates." Nature neuroscience 14.3 (2011): 387-397. PubMed PMID:...
  17. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...scientists was Nature. Overall scientists tweet URLs with scientific domains <10% of the time. Figure 3: Top ...by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure 4: Top Scientific Domains...have the most Twitter users. Biologists rank #8. Figure 1: Highly Represented Scientific Disciplines on...scientists on Twitter are male and 40% are female Figure 2. Gender Ratio of Scientists on Twitter. 60% of...scientific domains more often than other scientists   Figure 5: Portion of Tweets with Scientific Domains, Categorized...their fields than scientists outside their fields Figure 6: Follower Network of Scientists on Twitter. Each...
  18. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441(7097): p. 1061-7. PubMed PMID: 16810240...cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006. 126(4): p. 663...human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad...differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Cell Res, 2009...pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. 105-9. PubMed PMID: 21151107...Differentiation of human induced pluripotent stem cells to mature functional Purkinje neurons. Sci Rep, 2015. 5: ...Dedifferentiation, transdifferentiation, and reprogramming: future directions in regenerative medicine. Semin Reprod...
  19. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    Published
    Dec. 14, 2020, 2:15 p.m.
    ...updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation...can be measured by a BRET2 ratio, which is the ratio of GFP2 emission to RLuc8 emission (see figure). What...? The TRUPATH collection allows researchers to measure the physical association of combinations of different... signaling pathways.  This dissociation can be measured using a technique known as Bioluminescence Resonance...Rluc8 was more challenging. The Roth lab used structure-guided protein engineering and experimental refinement...both antagonists and inverse-agonists could be measured using the TRUPATH system. Conclusions Although...
  20. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...post about workplace bullying featured on the always interesting Tenure, She Wrote blog. Unfortunately...little pre-work most PhD students and postdocs do to ensure the advisors they choose will be ones that help...choose a field that you may want to work on in your future career, so the research topic will be more important...scientists to change labs – do as much as you can to ensure a good fit in advance. When a lab is interviewing...interviewing the lab to find a good “fit” and to ensure the PI will be a good role model and teacher. Do...creates a level of familiarity and confidentiality to ensure more honest answers. Ask people who work in the... to speak to them more than once to get a full picture and listen for hints at the same concerns from ...
Showing: 241 - 260 of 886 results