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We narrowed to 642 results for: Vars;

Showing: 241 - 260 of 642 results

  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...10% of DNA repair, with this rate varying across cell types. Variance can be caused by cell cycle rates...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

  2. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...one used in Luxturna™, can efficiently transduce various types of retinal neurons. Nowadays a toolbox of...serotypes are currently used in clinical trials, a variety of engineered ones have shown great promise in ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

  3. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type...transduced with multiple viral particles and labeled a variety of colors (figure 4b). But notice in figure 4b ... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...

  4. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...PspCas13b-ADAR2DD(E488Q) displayed robust editing with various spacer lengths from 30-84 nucleotides, and designated...targeted 34 pathogenic G->A mutations from the ClinVar database. They successfully edited 33/34 sites ...in therapeutic applications, the authors tested various dRanCas13b truncations that would allow packaging...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ... new cutting edge science, researchers of all varieties must stay current with literature and emerging...clear that while several people across teams had various levels of experience with bioinformatics and HTS...welcome to ask any questions they have.  These variations are suited for different applications and should...

  6. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...scientists understand how our vectors perform in various experimental settings. It is a curated set of technical...the knowledge base of AAV vector performance in various brain regions and cell types. Power in numbers ...Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations...

  7. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...lacZ reporter gene (3, 4), there are now many variations, including many that use auxotrophic rescue genes...provides a baseline level of reporter activation. Vary the expression levels of bait and prey proteins ...your protein are screened for binding. Using a variety of expression levels and systems can alleviate ...

  8. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools... efficiency, purity, and indel rate of each tool vary considerably. And there’s so much more exciting ...models-assisted optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing...

  9. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...still waiting for an indication of what (most of) Harvard, Johns Hopkins, (most of) MIT and the University...sciences. The Dana Farber Cancer Institute (at Harvard) and MIT BCS (Department of Brain and Cognitive...Boston Children’s Hospital is the only part of the Harvard group that has responded to the FLSA at the time...

  10. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...mouse lines that could be used to study a wide variety of phenomena, it’s possible that we’ll learn much...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...cell lines can become cross contaminated and are invariably much older than those available from biorepositories...thaw a new batch. The exact number of passages can vary between cells lines, as this can be dependent on...culture research experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...researchers feel they lack formal training in a variety of transferable skills. At Addgene we're helping...5 mental mistakes that kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from...brief exercise to spur innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out... people outside the department to accomplish a variety of goals. For example, I worked with some microscopy...current job at Addgene I work with people from various organizations in science and beyond. For example...

  14. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ... knowledge they require. Biotechnology companies vary to what extent they hire experts for their technical...complain about high background. In many instances, variations of the product’s specific application instructions...support roles A job in technical support can add a variety of skills to your CV and lead to other job opportunities...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...use Gateway to clone a gene of interest into a variety of expression systems. Keep reading to learn more...ready-made entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the...Addgene. These can be used to express genes in a variety of model organisms. Use the links below to find...

  16. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...inert green, cyan, yellow, and blue variants that can be used in various combinations. For example, with ...one of the most inert solutions for imaging in a variety of cellular compartments including the secretory...

  17. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... Mammalian Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...

  18. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...pragmatic adoption (although it might be argued various authors have simply chosen to submit elsewhere)...effort. Our standards of data recording are highly variable and usually left to individual researchers. Yet... said to be representative or the statistical variation is calculated and placed in the legend. Widely...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...beat in terms of size. Like CRISPR, there is a variety of small RNA types to choose from and can be catered...translation machinery. The composition of the RISC varies, but at the very minimum requires an Argonaute ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...

  20. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...common housekeeping proteins are influenced by a variety of experimental conditions (Ghosh et al., 2014)...that expression of your reference protein does not vary under your experimental conditions. Otherwise, you...control won’t be strongly influenced by unexpected variations in any individual protein. This ensures you are...
Showing: 241 - 260 of 642 results