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...community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular Posts of 2014 ...year in science – as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just ...

...plasmids you’d like to deposit with Addgene, be sure to visit addgene.org/deposit. Who knows, maybe yours ... articles cite the use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad...searchable list here). Tips for using pMD2.G At Addgene, we use pMD2.G for producing our ready-to-use lentivirus...Lentiviral Transduction Additional Resources on Addgene.org Check out our Viral Service Learn more about...

...promoter in rat. Contribute your AAV data Just like Addgene’s plasmid repository, the AAV Data Hub is a community-driven...only accepting data for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, ...accelerate your research. Sincerely, Melina & The Addgene Team ...

...Additional Resources on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages... between tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial...poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma model for ...with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...: Deciphering the Relationship Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids...

...tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098...cardiovascular disease. Find the LipoGlo plasmids at Addgene! Lawler et al, QJM: An International Journal of...systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications, 2019. PubMed...worms and have made these constructs available at Addgene. These include Arch(D95N) and QuasAr, as well as...biosensors at Addgene. Hashemi et al., PNAS. 2019, PubMed PMID 31371514. ... Various Addgenies...

...adeno-associated viral (AAV) vectors available at Addgene are suitable for in vivo studies, our scientists...measuring viral titer and assessing purity. But Addgene is also conducting more rigorous quality control...plexWell technology, we can now perform NGS at Addgene to sequence all the DNA packaged inside the viral...AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation, limitations,...commonly performed for research-grade materials. Addgene is going the extra mile and pioneering the systematic... Press, pp 247–278 Additional resources on the Addgene blog Check out our Viral Vectors Featured Topic...discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality control...

...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...

...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...Used in Cancer Lineage Tracing Resources on Addgene.org Visit our Cancer Collection Pages Deposit Your...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional...Additional Resources from Addgene Find more management resources in our Mentoring for Scientists Series ...

...molecular weight ladders were recently deposited with Addgene. A team of undergraduate students led by Dr. Song...pPSU1 and the pPSU2 plasmids are available from Addgene. Learn How to Analyze a Restriction Digest References...Central PMCID: 5446395. Additional Resources on the Addgene Blog Are you a Frugal Scientist? Save Money with...the Evolution of Lab Techniques Resources on Addgene.org Use the Kit-Free Alkaline Lysis Plasmid Miniprep...

...talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell...blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid...designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list...sequences coming soon!  Additional Resources on the Addgene Blog How to verify your plasmid Check out our ...featured topic page Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular...

...thread ⬇️⬇️⬇️ — Addgene (@Addgene) April 15, 2020   Additional resources on the Addgene blog Read about...essential research into COVID-19 (like here at Addgene!), and new databases are supporting their access...guide for external collaborations Resources on Addgene.org Browse our COVID-19 plasmid collection ...

...sometimes unclear or missed altogether. Emailing Addgene might not be as high on the published-paper-celebration...in this status — a win-win situation for all. Addgene will release plasmids on hold if they are clearly...distribute unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share... for reading, and for depositing plasmids with Addgene! We'll continue to review our policies to make ...celebrate!), send us a quick email at deposit@addgene.org with the citation and your deposit or plasmid...website, Help Center, or email us at deposit@addgene.org. ...

...resources at Addgene Check out Addgene’s plant vectors! Find the plasmids from this paper at Addgene. Other...as plasmid yield during preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile...

... Find plasmids from this publication at Addgene.   Addgene’s Fluorescent Protein Plasmids and Resources... GFP is great news for microscopy enthusiasts! Addgene has empty backbones containing GFP variants used...PubMed. Find plasmids from this publication at Addgene. Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis...

...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business.... I think these are things that I can bring to Addgene -- that comfort with saying, “if we do this, then...

... wide range of fields from Addgene. What you might not know is that Addgene distributes curated collections...to deposit a kit, please contact us at depost@addgene.org. ...

...workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address irreproducibility... to Teytelman’s connection with Joanne Kamens, Addgene also had the opportunity to contribute to this ...anyone could present the material. For example, Addgene’s contributions by Tyler Ford included reagent sharing...Tsang for editing. Additional resources on the Addgene blog: Check out this guide on Transferable Skills...workshops Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific...

...Resources on Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols...needed to expand the HEK293 cells. Check out the Addgene AAV Production protocol for more details. Pro tips...because I don’t have enough AAV. Compared to the Addgene AAV Production protocol, I take a lazier approach... the qPCR run and data analysis. Refer to this Addgene AAV Titration by qPCR Protocol for more details...PubMed PMID: 18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool ...

...editing LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping ...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While...  New AAV6 and AAV11 packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort...effort to expand your gene delivery toolkit, Addgene now offers packaging plasmids for AAV6 and AAV11! Why...projection neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses... viral vectors ready to use in your lab. Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ...