We narrowed to 319 results for: bre

1. Plasmids 101: The Wide World of Natural Plasmids

   Type

   Blog Post

   Published

   Oct. 8, 2024, 1:15 p.m.

   ...coli is safe to use in the lab but also causes outbreaks of food poisoning, the difference is virulence...

2. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ... the Fragmid system, the Fragmid website, which breaks the design process down into four easy steps, provides...

3. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks disulfide bonds HCl Adjust pH to 6.7–6.8 ...

4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

   Type

   Blog Post

   Published

   Sept. 13, 2016, 2:30 p.m.

   ...Howell -Founder of Howell Communications Philip Brenner, PhD - Assistant Professor of Sociology. University...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

   Type

   Blog Post

   Published

   Dec. 1, 2015, 3:30 p.m.

   ... Addgene depositor) Craig Mello. Taking his groundbreaking work in RNAi one step further, his lab is now...

6. The Materials Science of Optogenetics Experiments

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   Blog Post

   Published

   Sept. 17, 2015, 2:30 p.m.

   ...location of your region of stimulation (the z-axis bregma coordinate). Because the light that is emitted ...

7. How-to: 5 Steps to a Great Panel Discussion

   Type

   Blog Post

   Published

   Oct. 22, 2014, 1:57 p.m.

   ... follow and gets things rolling. It can make or break the discussion section. And what about those audience...

8. PhD Applications After COVID

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   Blog Post

   Published

   Jan. 5, 2023, 2:15 p.m.

   ...and video communication. But first, take a deep breath; at least you know you have an answer to that previous...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ... Vlieger D, Corbett KS, Torres GM, Wang N, Van Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S...

10. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the separation...

11. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...methods that make working with multiple inserts a breeze. Among them are some of the homology based methods...

12. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two...

13. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...patience, as you wait for the anesthetized mice to breathe in the liquid. Restraint: anesthesia Location: ...

14. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...effectors. Nucleases generate a classic double-stranded break, while modulators regulate transcription and chimeric...

15. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ...) everything ourselves and it’s a hard habit to break. But as a manager you will be quickly overwhelmed...

16. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ...sequence of events plays itself out: The researcher breaks eye-contact with any other human in the room. Their...

17. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...binding the protospacer. The resulting double strand break (DSB) stimulates DNA repair mechanisms that can ...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    .... However, if the molecules are confined to a subregion of the cell and/or they cannot tumble in 720°,...

19. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ...to get recharged. The fun change of pace helped break up the negativity and allowed me to focus on the...

20. Genome-wide Screening Using CRISPR

    Type

    Blog Post

    Published

    Aug. 18, 2015, 2:30 p.m.

    ...-type Cas9 and a gRNA generates a double-strand break in the target DNA, which, when repaired through ...