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We narrowed to 800 results for: cha;

Showing: 241 - 260 of 800 results

  1. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...results. GBEs seemed to follow the same initial mechanism as in mammalian cells — by excising the freshly... A's, producing C → A edits. Figure 3: Mechanism of cytosine transversion editors. Red arrows indicate...agreed on the first part of the likely editing mechanism: cytidine deaminase converts C to U, and UNG excises... Base Editing terms   Base transition Base changes between purines (A ↔ G) or between pyrimidines ...pyrimidines (C ↔ T).   Base transversion  Base changes from purine (A or G) to pyrimidine (C or T) or vice-versa...Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org...

  2. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... virus used. All of these expression control mechanisms require a specific modification of the viral ... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...Viral vectors are being explored as the delivery mechanism for increasingly popular nucleic acid-based vaccines...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...1742-4690-4-66. PMID: 17888184; PMCID: PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the...

  3. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...open-source microscopy seeks to change this. This recent push gives users the chance to understand the "black...much more involved. Challenges such as the provision of an accessible supply chain for parts that are ...Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative ...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... the materials in our catalog are very well-characterized. During this time, we’ve also created a variety...this, throughout the year we rolled out multiple changes to our website, including the recent homepage and... a look at the least visible part behind these changes: the backend technology that powers the new website...granularly customize the search engine, and a scoping mechanism that allows category-specific, narrow searches...among others. We also created a normalization mechanism to “collapse” variations and synonyms of the same...

  5. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...designed by the Toettcher Lab, OptoNBs are photoswitchable engineered nanobodies whose binding of their...small (~15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....Optogenetic control of protein binding using light-switchable nanobodies." BioRxiv (2019): 739201.  Additional...

  6. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... in D.C. for a few years, Susan was ready for a change of scenery. She moved to Seattle and continued ...to articles for the Fred Hutch website, social channels and Hutch magazine. Getting pulled in all of these...finding that uninterrupted time to be creative is challenging,” says Susan.  Another difficulty is that some... scientist or someone who’s affected by climate change or affected by a superbug. I think we solve these...you enjoyed this series, subscribe to the Career Channel of the blog so you don’t miss out on future career...

  7. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans S., 2013). They...antibody: An ~150kD protein with both light and heavy chains made by immune systems. But here, we're not interested... or less immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~27kD engineered ...They are comprised of the variable heavy and light chain regions, connected by a short linker peptide. The...

  8. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ...matter of applying that framework to the myriad challenges we encounter. As you spend time in a lab learning...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...yourself if you missed any important details. Or challenge yourself to think of more creative solutions. ... know-how and more about how you approach new challenges.     Know your strengths! Research experience...employer that you are well equipped to work through challenges from day one in your new position. Of course,...

  9. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...go ahead with raises. What will these further changes mean? The abrupt reversal in salary raises at institutions...Continuing efforts to gather information and expedite change We are continuing to update our FLSA and Postdocs..., under “How institutional plans have/have not changed since the injunction”. Postdocs around the country...enterprise and promote their voices in driving changes in science. Follow him on Twitter @BiophysicalFrog...the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages Read ...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...viruses yield dsRNA through a slightly different mechanism thought to be associated with bidirectional transcription...knew about all the diverse in vitro production mechanisms. siRNA, also known as RNAi, are short, dsRNA ...and dysfunction, it is unclear what the precise mechanism behind the production of these dsRNAs is. A major...response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation are...levels, possibly due to dysregulation of removal mechanisms, an array of diseases can result (Fanconi Anemia...

  11. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...Sauermann, Henry, and Michael Roach. "Science PhD career preferences: levels, changes, and advisor encouragement...would be the effects, positive and negative, of changing the workforce structure? Helping to answer some... the following panel members: Jonathan Dinman, Chair of the Cell Biology and Molecular Genetics Department...collects data on the careers of science PhDs. Michael Teitelbaum is a Senior Research Associate at the...

  12. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...person in the group for your needs. Beth Schachter of Beth Schachter Consulting is part of that group and ...are for the coaching relationship. Do you want to change jobs? Grow your professional presence? Learn a ...and make direct suggestions for improvement and change. Work with the coach to define how you will know... Polk of From PhD to Life. She runs great tweet chats, has an outstanding blog and, while her PhD is in...

  13. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...and has provided clues to their previously uncharacterized cellular roles. In the current phase of NURSA... expression and reporter plasmids and the free exchange of these vectors between laboratories. The development...example, was a key development in the functional characterization of NRs and coregulators, and although the ...Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular and Cellular Biology...Nuclear Receptor Signaling.   Further Reading: Chawla, A., Repa, J. J., Evans, R. M.and Mangelsdorf, ...

  14. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...base-to-base conversion in principle, but some changes require multiple steps and enzymes. Prime editing...can encode any of the 12 possible base-to-base changes in a single edit. Prime editing is also more precise...breaks has been widely used to generate precise changes. However, the efficiency of Cas9 cleavage is relatively....2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza, R. M., Martin...J., Pierce, S. E., Tolar, J., Meissner, T. B., Chaikof, E. L., Osborn, M. J., & Liu, D. R. (2024). Efficient... L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R....

  15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T... to blue light, CRY2 undergoes a conformational change that permits it to bind to the CIB1 (cryptochrome-interacting...optogenetic system (OptoSTIM1), caused two-fold faster changes in intracellular Ca2+ levels than the original ...techniques for live-cell imaging of the dynamic changes in biochemical activity within the cell. Their ...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...strangers to cloning. If you’re reading this, then chances are you have been in the cloning trenches yourself...specific functions. Most individual parts have characterized activities with predictable behaviors. While...part in different contexts and plug different characterized parts into a TU! What is modular cloning useful...

  17. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...insertion, inversion, translocation or cassette exchange. By utilizing recombinases to manipulate genomes...Optogenetic tools use light to induce conformational changes in a protein.    Cells expressing an RFP...crRNA-guided DNA cutting, unlike other well-characterized Cas enzymes, which rely on multiple active sites...AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons...use viral prep in serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...’s native locus, but introducing large genetic changes in C. elegans with CRISPR can be difficult since...her colleagues at Yale University fused a color-changing fluorescent timer (FT) protein to the histone ...synthesis and, after a few hours, a conformational change causes the protein to fluoresce red. H2B-FT-red...a Cre-dependent, soma-targeting inhibitory channelrhodopsin. It’s now available as AAV5. Find the AAV...

  19. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...work,” she said. “School will always be there.” Changing your path! It’s okay! Our blog writer, Beth Kenkel...research and technical assistant, when her life changed––she had a daughter. Needing a career switch because...thought, ‘Let me pursue this, let me make a slight change so I can have flexibility and be up to date with...Bioinformatics it is.’” Though she misses the bench, the change gave her the balance she needed. It is incredibly...incredibly helpful to me to know that you can make big changes in your career path and still find comfort and ...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... plasmid via sequencing or restriction digest. Chances are, you’ll even have DNA left over for other applications... the culture medium. Improve the culture media Changing to a richer media or adding supplements can improve... low copy plasmids or slower growing strains, a change in media may be advantageous. Reduce the antibiotic...oxygen for optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired...shaker speed is fast enough for sufficient gas exchange in the overnight culture. Generally, large cultures...
Showing: 241 - 260 of 800 results