We narrowed to 372 results for: ena
-
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity and sgRNA...
-
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m....researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog PostPublishedAug. 6, 2024, 1:15 p.m....variant, Phoenix-gp, contains only Gag-Pol and enables additional flexibility in pseudotyping. ... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....isotype control for that particular marker. This enables you to identify any non-specific binding to know... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....upgrades to our inventory management systems to enable efficient, tracked production and distribution ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m.... various serotypes available (AAV2, AAV5, AAV8) enable different patterns of transduction and transgene... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m....sharing speeds science, and our entire mission is to enable that process. Our repository of plasmids, AAVs,... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split... -
Antibodies 101: ChIP
TypeBlog PostPublishedMarch 10, 2022, 3:25 p.m....native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m.... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m.... that template for repair. By using these two scenarios, you can either knock out a gene or knock in a... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m.... Broadening the spectral diversity of GEVIs can enable resolving single action potentials and further ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....the cells with a PP1/PP2A inhibitor leads to maintenance of phosphorylated MEK1/2 levels, suggesting PP1... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....techniques. Gel filtration and SDS-PAGE under non-denaturing conditions separate proteins based on size. Whether... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...