We narrowed to 767 results for: form

... the anti-sense strand, then the ssDNA template formed will be sense. Therefore, mutagenic oligos need... plasmid. The ssDNA template is generated by transforming a backbone phagemid vector into an E. coli strain...to remove residual starting template. After transformation and harvest, the library is ready for use. ...true if the sense strand is nicked for template formation: template will be anti-sense, primers should be...ease of use, 2) time savings, and 3) ability to perform single site or multi-site saturation mutagenesis...

... when bound to DFHBI. It’s also smaller than any form of Spinach - only 49 nucleotides in length - and...aptamers are used to track cellular processes by transforming cells with plasmids encoding aptamer-tagged ...UTR of mRNA that bind metabolites, leading to conformational changes that control the expression of coding...expression vectors to create a library. Bacteria were transformed, and then sorted by fluorescence-activated cell...

...additional cofactors or using significantly truncated forms that limit its functionality. To make biophysical...C-terminus (or even in the middle of proteins) and performs well in many different antibody-based applications...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...indels after DNA repair. B) M. abscessus is first transformed with Cas9 on an integrative plasmid containing...resistance selection marker KanR. This strain is then transformed with the sgRNA cassette on a plasmid expressing...

...CD11c-. Finally, B cells are CD11c- and CD123-, forming a population in the lower left(ish) corner.  If...learning how to read a gating strategy.  Other information FACS plots often contain other data. Some will...Abdulaziz University, Kingdom of Saudi Arabia. Bioinformation, 14, 53–59. https://doi.org/10.6026/97320630014053...

...causes cells that it infects to fuse together and form syncytias that cause the cells to die. Cytotoxicity... of these review articles to find tables and information on different viral membranes, tropism, and advantages...

... pDonor carries a customizable cargo gene in the form of RE-cargo-LE (RE, right end; LE, left end) pTnsABC...in E. coli BL21(DE3) as transformants failed to grow after the transformation of pTargetF into the BL21...strand breaks it introduces makes it difficult to perform two or more integrations on the genome. Most recently... This would result in the lack of recovered transformants. Our speculation motivated us to update the ... sequence in pEcgRNA. Next both plasmids are transformed in E. coli with the donor DNA for editing to ...DE3) that resulted in a lower efficiency of transformation for pTargetF series plasmids. Fortunately, ...plasmids (pDonor and pQCascade) with 4 rounds of transformation to complete the N-acetylglucosamine strain ...

...genes and proteins coordinate billions of neurons to form the functional networks of the human brain.     ...Buchholz, Angrand, and Stewart 1998). FLPe’s performance was further improved, by adding a nuclear localization...technology. Read the genOway press release for more information. References 1. Atasoy, D., Y. Aponte, H. H. Su...

... random amino acid sequences or (ii) designed to form an inert, unstructured peptide and therefore consisted...by proteases. Nowadays, with ample structural information available, it’s clear that the C-terminus of ...the POI would work best. Ideally, structural information can guide this decision. In our experience, the...-Hermans et al., 2011).  Although structural information may guide design, it is advisable to generate...

...Instead, they incorporate a truncated, non-fluorescent form of YFP as the default state of the transgene such...

... your manuscript is a form of (hopefully constructive) conflict. As you perform the experiments to address...trouble spots:  Communication (or lack thereof): Information sharing gets more challenging the bigger an organization...

... 2017), but requires an oxidizing environment to form. When expressed in the non-oxidizing cytoplasm of... at a distance that favors proper folding and formation of the antigen-binding site while also minimizing...functional scFvs; however, some individual scFvs performing better in one configuration than the other (Sandomenico...challenge of bacterial expression is the proper formation of the disulfide bond between the VL and VH domains...of bacteria to allow for proper disulfide bond formation.  expression in strains of bacteria that are redox...Sandomenico et al., 2020). conversion to a cysteine free format that’s easier to express in the cytoplasm of E....scFvs designed for oncology applications are reformatted as bispecific molecules which bind CD3 and a ...

...representation of integration process and episomal forms of non-integrating transgenes. Created with BioRender.com...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...

...restriction digestion to anneal to one another and form a complete, circular plasmid. You then add DNA ligase...You should perform, at minimum, two transformations after a ligation: 1. Control Transformation containing...contamination from uncut plasmid. 4. Transformation Transform your ligation reaction into your bacterial...Read on for an in-depth breakdown of how to do perform restriction digests.   Overview of the restriction...Bromide Pre and Post UV Light 0.5 For more information on these stains see the Bitesize Bio Blog and... how many colonies you should expect in the transformation due to background re-circularization and contamination...competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction into competent...

...brain, a connectome that would detail how neurons form circuits and where they synapse. View the overview...

... molecules that cannot function in isolation but form a fully functional Cas9 upon dimerization. Splitting...inactive dCas9 and VP64, respectively. CibN and Cry2 form a heterodimer in response to blue light, which will...this system if a small contaminating PCR product formed from primer dimers and containing the missing codons...limitation to this strategy is that each "bit" of information must be controlled independently; that is, the...the signal to record one bit of information must not act on any of the other bits in order to achieve ...capable of recording 11 bits (1.375 bytes) of information. This leads to 2048 (211) possible combinations...designed to minimize the possibility of dimer formation. Prosser et al., Biotechnol Lett. 2015. 37:383...

... available from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: ... available from Rudolf Jaenisch’s lab, with the former also available as ready-to-use lentivirus. ...ME, et al (2018) Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome...

...feel you rolling your eyes, but even if this ideal form of pre-registration doesn’t come to fruition any... it easier to find information about research tools. BenchSci collates information about antibodies while...can find a variety of additional resources and information about organizations promoting reproducibility...S. Flier, Researcher at Harvard Medical School, former dean of the faculty of Medicine at Harvard University..., generating new therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility...reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers should be able to follow... tools that make it easier to share in-depth information about reagents, protocols, analysis tools, and...

... or alternative splicing that could lead to some form of the target protein to be present and recognizable...your antibody performs as expected in one application does not mean that it will perform as expected in...so you use your bioinformatics skills (or your friendly neighborhood bioinformatician) to look at RNA ...doesn’t necessarily mean that you yourself have to perform extensive validation experiments for every antibody...data, you may need to roll up your sleeves and perform some extra validation experiments yourself.  Goal...researchers can use to show that their antibody performs as expected. One of the benefits of these pillars...levels tend to correspond to protein levels, so you perform immunohistochemistry with your antibody on these...

...crosslinking or tilted-stage imaging. A truncated form of a tardigrade LEA, RvLEAMshort, protected samples...PASSIGE efficiency (Pandey et al., 2024). The best-performing variant yielded an average integration efficiency...

...expressing the protein of interest fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody... Their soma-targeted variant SomaFRCaMPi also performed substantially better than other soma-localized...biomolecular condensate in human cells — causes a rare malformation syndrome (Mensah & Niskanen et al., 2023). Now...