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We narrowed to 486 results for: multi

Showing: 241 - 260 of 486 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...inherent in Nextflow’s design, we were free to use multiple programming languages (Python, Perl, Java, R, ... adaptable property gave us the freedom to use multiple open-source sequence assembly tools at the appropriate...

  4. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ... the more complex Class I systems that contain multiple subunits, but the more they worked with it, the...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  5. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...as those containing cassettes of the same gene multiple times.  Bioinformatics at Addgene After a run ...assembly. Geneious will show how reads align, reveal multiple contigs, and highlight sequence identities in ...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...interdisciplinarity index of research for single or multiple disciplines – you can select the disciplines and...networking, visual appeal, instant sharing across multiple other sites), I created a new platform for sharing...

  8. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII ... Proteins which contain hydrophobic regions or multiple disulfide bonds may aggregate and become insoluble...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...behavioral geneticists may track the motion of multiple animals on different occasions via video, generating...limiting numbers of figures such that many have multiple, complex panels. PLOS, itself, has not finalized...

  11. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment. Zebrafish...making it easy to conduct large experiments over multiple generations. Similarly to other model organisms...

  12. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...obtaining homo- and heterozygous mutations by multiplying the single allelic mutation incorporation probabilities... systems.     References 1. Cong, Le, et al. "Multiplex genome engineering using CRISPR/Cas systems." ...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ... words, any insertion or deletion that isn’t a multiple of three will do. A popular screening method for... Multiple Authors...

  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...every communication that gets sent out through multiple channels is not reasonable or sustainable. That...will be gray areas that either a) overlap with multiple teams or b) fall into a gap between teams that...

  15. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... scrambling. Everything we had set up involved multiple people in tight locations. Conference rooms for...to the talent’s shirt. But knowing I would have multiple interview subjects, I positioned a boom mic just...

  16. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...be packaged in AAV vectors, potentially with multiplexed targets.   Figure 4: CHARM uses a fusion...  Multiple Authors...

  17. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...These methods can include labeling markers with multiple proteins/antibodies, targeting markers found within...Otherwise, your secondary antibody will bind to multiple primary antibodies, yielding a false positive ...

  18. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Pfeiffer P, et al. Mutagenesis (2000). PubMed. Multiple Pathways of Recombination Induced by Double-Strand... Multiple Authors...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... any gene of interest, one should require that multiple gRNAs of different sequences give rise to the ... in the interpretation of results by requiring multiple sequences targeting a gene to score in order to...

  20. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...primary cellular receptor and sometimes one or multiple coreceptors, which may be present on different... AAV8 AAV8 has high transduction efficiency in multiple tissues, rapid transgene expression, and can cross...
Showing: 241 - 260 of 486 results