We narrowed to 264 results for: nif
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TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....clones, it’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...
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Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....figure). Template A high purity plasmid prep significantly increases the success rate of site directed ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog PostPublishedMay 10, 2016, 2:30 p.m....-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog PostPublishedMarch 1, 2016, 3:30 p.m....they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m.... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard... -
Hot Plasmids: Spring 2025
TypeBlog PostPublishedJune 3, 2025, 1:15 p.m....and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity... -
Antibodies 101: Multiplex Immunofluorescence
TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog PostPublishedMay 18, 2018, 7:32 p.m....few replicates leading to suspect statistical significance (you can read more about this problem in Button... -
Plasmids 101: Restriction Cloning
TypeBlog PostPublishedFeb. 18, 2016, 3:42 p.m....recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you... -
A History of Genome Engineering in Popular Culture
TypeBlog PostPublishedFeb. 25, 2020, 2:15 p.m....10.1038/s41586-018-0070-x Griffith F (1928) The Significance of Pneumococcal Types. Journal of Hygiene 27... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....depending on how and where you pursue it. It can manifest via research and data gathering, regulatory efforts... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog PostPublishedMarch 6, 2018, 1:49 p.m....However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....should appear less green. Note that there won’t be uniform editing across all the cells in the population,... -
Typing CRISPR Systems
TypeBlog PostPublishedMarch 18, 2025, 1:15 p.m....like to explore Class 2 CRISPR in more depth, the Jennifer Doudna lab maintains an encyclopedia of Class ... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog PostPublishedApril 25, 2017, 2:30 p.m....detail. References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for... -
Antibodies 101: Introduction to Antibodies
TypeBlog PostPublishedJan. 19, 2021, 2:15 p.m....antibodies. They also have the advantage of being significantly smaller, meaning they can recognize epitopes... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....purification columns we’ve been talking about!) are a significant source of waste in laboratories. To help combat...