We narrowed to 266 results for: nif

...double-stranded breaks in the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR...

...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...

...group (15) While marchers are a group with one unifying interest (the advancement and inclusion of science...

...figure). Template A high purity plasmid prep significantly increases the success rate of site directed ...

...-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ...

...they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ...

...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...

...few replicates leading to suspect statistical significance (you can read more about this problem in Button...

...and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity...

...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

...depending on how and where you pursue it. It can manifest via research and data gathering, regulatory efforts...

...10.1038/s41586-018-0070-x Griffith F (1928) The Significance of Pneumococcal Types. Journal of Hygiene 27...

...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...

...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...

...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...

...like to explore Class 2 CRISPR in more depth, the Jennifer Doudna lab maintains an encyclopedia of Class ...

...detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for...

...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...