We narrowed to 265 results for: nif

1. Antibodies 101: Normalization and Loading Controls for Western Blots

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   Blog Post

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   April 8, 2025, 1:15 p.m.

   ...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...

2. March for Science

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   Blog Post

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   April 21, 2017, 2:30 p.m.

   ...group (15) While marchers are a group with one unifying interest (the advancement and inclusion of science...

3. Site Directed Mutagenesis by PCR

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   Blog Post

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   Aug. 2, 2016, 2:30 p.m.

   ...figure). Template A high purity plasmid prep significantly increases the success rate of site directed ...

4. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

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   May 10, 2016, 2:30 p.m.

   ...-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy number, although the ...

5. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

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   March 1, 2016, 3:30 p.m.

   ...they all start with a G!) but actually work by significantly different methods. Golden Gate cloning does ...

6. How to Negotiate a Successful Lab Start Up Budget

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   Blog Post

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   Sept. 1, 2015, 2:30 p.m.

   ...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

7. Plasmids 101: Common Lab E. coli Strains

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   Blog Post

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   Nov. 7, 2014, 2:56 p.m.

   ... Inactivating mutations in specific genes are signified with a minus sign (-) as is typically standard...

8. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

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   May 23, 2023, 1:15 p.m.

   ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...

9. Hot Plasmids: Spring 2025

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   Blog Post

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   June 3, 2025, 1:15 p.m.

   ...and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, and sensitivity...

10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    Blog Post

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    May 18, 2018, 7:32 p.m.

    ...few replicates leading to suspect statistical significance (you can read more about this problem in Button...

11. Plasmids 101: Restriction Cloning

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    Blog Post

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    Feb. 18, 2016, 3:42 p.m.

    ...recipient plasmid self-ligation, you will see significantly more colonies when you add ligase. If you do...

12. Finding Your Science Policy Path

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    Blog Post

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    Sept. 22, 2020, 1:15 p.m.

    ...depending on how and where you pursue it. It can manifest via research and data gathering, regulatory efforts...

13. A History of Genome Engineering in Popular Culture

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    Blog Post

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    Feb. 25, 2020, 2:15 p.m.

    ...10.1038/s41586-018-0070-x Griffith F (1928) The Significance of Pneumococcal Types. Journal of Hygiene 27...

14. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

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    March 6, 2018, 1:49 p.m.

    ...However, some ligases can ligate mismatches to a significant degree, and very active ligases, such as T4 DNA...

15. Important Considerations When Using AAVs

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    Blog Post

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    June 13, 2017, 2:30 p.m.

    ...the cell type under study. Many AAVs will show significant expression in a variety of cell types, but you...

16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post

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    March 7, 2017, 3:30 p.m.

    ...should appear less green. Note that there won’t be uniform editing across all the cells in the population,...

17. Typing CRISPR Systems

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    Blog Post

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    March 18, 2025, 1:15 p.m.

    ...like to explore Class 2 CRISPR in more depth, the Jennifer Doudna lab maintains an encyclopedia of Class ...

18. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

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    Aug. 15, 2017, 1:24 p.m.

    ...incremental change of the excitation wavelength can significantly affect emission intensities from fluorescent...

19. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

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    April 25, 2017, 2:30 p.m.

    ...detail.   References 1.Patterson, George H., and Jennifer Lippincott-Schwartz. "A photoactivatable GFP for...

20. Antibodies 101: Introduction to Antibodies

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    Blog Post

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    Jan. 19, 2021, 2:15 p.m.

    ...antibodies. They also have the advantage of being significantly smaller, meaning they can recognize epitopes...