We narrowed to 363 results for: nsf

1. Reflections and Looking Ahead

   Type

   Blog Post

   Published

   Feb. 25, 2025, 2:15 p.m.

   ...simplifies your viral vector options. Almost any AAV transfer plasmid in our repository can now be packaged ...

2. Bringing Sustainable Practices to the Lab: Innovations

   Type

   Blog Post

   Published

   March 7, 2019, 2:39 p.m.

   ...your RMV to the landfill, it’s collected and transferred to the Red2Green processing facility, where it...

3. London Calling - Addgene’s New Europe Office in London

   Type

   Blog Post

   Published

   June 10, 2014, 3:20 p.m.

   ...spondylarthritis, using Bioluminescence Resonance Energy Transfer (BRET) technique, and plasmids to study the expression...

4. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ...target cells that have previously been stably transfected for Cas9 expression. The Yusa lab has used their...

5. Antibodies 101: Affinity Tags

   Type

   Blog Post

   Published

   Sept. 19, 2023, 1 p.m.

   ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...

6. Oh, The Places You Can Go: Careers in Science Communication - Product Development

   Type

   Blog Post

   Published

   Oct. 18, 2017, 1 p.m.

   ...measure, here’s a few other posts about developing transferable skills while in grad school Resources on Addgene.org...

7. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   Published

   Sept. 14, 2017, 12:40 p.m.

   ...Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9 or SpCas9 RNP’s and...

8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

   Type

   Blog Post

   Published

   June 5, 2017, 3:19 p.m.

   ...One big reason is that scientists and technology transfer offices trust us to work for them with full transparency...

9. Fluorescent Proteins 101: Fluorescent Biosensors

   Type

   Blog Post

   Published

   May 18, 2017, 2:30 p.m.

   ...triggers CaM/M13 interaction that increases FRET transfer between the fluorescent proteins. FRET is highly...

10. Plasmids 101: What is a plasmid?

    Type

    Blog Post

    Published

    April 2, 2020, 6:17 p.m.

    ...vector containing the newly inserted gene is transformed into bacterial cells and selectively grown on...

11. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type

    Blog Post

    Published

    July 2, 2015, 8:37 p.m.

    ...management, information distillation, etc) are transferable to many different fields. If you start thinking...

12. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...how much easier it makes our lives. Plasmid transformation into E. coli is a fairly inefficient process...

13. New Viral Vectors - Winter 2025

    Type

    Blog Post

    Published

    Jan. 28, 2025, noon

    ...Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure...

14. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...and enrichment use of XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry...

15. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type

    Blog Post

    Published

    June 27, 2018, 1:16 p.m.

    ...antibodies. These properties make nanobodies easier to transform and express in bacteria, which, in turn, makes...

16. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    Published

    May 9, 2019, 1:15 p.m.

    ...MettI7a1 encodes an uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α...

17. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...Alberta and University of Oregon created mTFP1 by transforming E. coli with a gene library of approximately...

18. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority...

19. The Strength of Story Telling

    Type

    Blog Post

    Published

    Dec. 19, 2017, 2 p.m.

    ...scientists in SciComm careers Check out our series on transferable skills ...

20. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...the targets that have the biggest potential to transform patients' lives. Previously Stegmeier was the ...