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Showing: 241 - 260 of 597 results

  1. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...to control their own future in science (see my previous blog on this).  Indeed, that was a primary message...rules and practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled...

  2. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...variety of careers outside of academia. She has had previous graduate students and postdocs go on to work in...what you are truly passionate about. Check out my previous post if you're looking for more information about...

  3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral infection into target cells that have previously been stably transfected for Cas9 expression. ... in bacterial toxin resistance, identifying 4 previously unknown genes.  “With this work, it is now possible...

  4. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...organism, all cells of that type are affected. Previous tools to overcome this restraint include an AAV-based...into an attB site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites...

  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...and the species they are compatible with are all reviewed here. What are affinity tags? Affinity tags are...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...

  7. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...bacterial chromosome that stores a genetic record of previously encountered foreign nucleic acids. The unusual...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/...

  8. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...the Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans...Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int...

  9. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages...

  10. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall...

  11. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...CRISPR construct needs to be segregated away to prevent continued production of new alleles or off-target... generation when CRISPR needs to be removed to prevent further editing, then green offspring can be selected...

  12. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...other hand, if it opens up an epitope that was previously hidden, an antibody might suddenly work that ...denatured, allowing the antibody to bind to a previously hidden epitope (right). Created withBioRender.com...

  13. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set...related versions that have different properties. To prevent misidentification, we augment the database with...

  17. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...context, it’s hypothesized that base removal helps prevent the invading genome from “recovering” its original...cannot switch guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it...

  18. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy...

  19. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535. Lederberg...Bacterial Plasmids: A Proposal.”Bacteriological Reviews 40.1 (1976): 168–189. PubMed PMID: 16350226. PubMed...

  20. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...obtained cells from patients with the condition to reveal some “outstanding differences.” They now have some... they now think that mutations in the gene may prevent neurons from returning to basal conditions normally...
Showing: 241 - 260 of 597 results