We narrowed to 432 results for: sequence

... predefined DNA sequence that uses trprimers and enzymes that will amplify a sequence only if present....

...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...

... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

...pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case study...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...

...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

...often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, fluorescence-based...method is often used preceding next-generation sequencing or microarray studies and is not used as much...

.... Different antibodies bind different protein sequences called epitopes and scientists can use these to...different targets, the scientists fused them to sequences called complementarity determining regions (CDRs...

...vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...

...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

.... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...