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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 430 results for: sequence

Showing: 241 - 260 of 430 results

  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...

  2. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...selection cassette with 5′ and 3′ flanking sequences matching the sequences upstream and downstream of the open...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...However, to allow for more room for regulatory sequences while still fitting Cas9 and its gRNAs into one... systems: St1Cas9 requires a very specific PAM sequence that limits the number of targetable loci, and...enrichment on streptavidin and next-generation sequencing). Using this sensitive method, they found that...

  4. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...edits (St. Martin et al., 2018). To circumvent sequencing and the need for a DSB in their previous reporter...need for single cell isolation and downstream sequencing (Standage-Beier et al. 2019). TREE is a real-...codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized that if they created...

  5. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...targeting system could be used to routinely deliver sequences of interest to cells with efficiencies of 1-40%...CRISPR is remarkably effective for introducing non-sequence specific frameshift mutations through aberrant...template. It is this application - the creation of sequence-specific knockins - for which the AAV approach...currently focuses on determining the mechanisms and consequences of cohesin gene inactivation in human cancer...

  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ... however, Cas9 allows you to easily change the sequence targeted by the activator by simply providing ... consist of plasmids that over-express coding sequences from a given cell type or organism, have been ...several popular CRISPR-based tools for altering DNA sequence or regulating gene expression.     References ...

  7. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case study...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...host machinery to make additional copies. ori sequences are generally high in As and Ts. Why, you ask?...that affect plasmid copy number  Although the sequence and regulation of the ori dramatically affect ...

  11. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    .... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

  13. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

  16. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

  17. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

  18. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

  19. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

  20. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...
Showing: 241 - 260 of 430 results