.... Different antibodies bind different protein sequences called epitopes and scientists can use these to...different targets, the scientists fused them to sequences called complementarity determining regions (CDRs...

...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...

...vectors and plasmids with repeating sequences. Repeating sequences within a plasmid can undergo intramolecular...

...discussion, we sent it for sequencing. The gene was successfully sequenced, confirming that we had made...

.... For example, Dave Grawoig is using nanopore sequencing at BosLab to perform proof-of-concept experiments... to collect various soil samples, extract DNA, sequence it, and analyze the data to characterize the microbiomes...

...downstream measurement of miRNA efficiency on a target sequence. The gene of interest is cloned into a plasmid...luciferase, until miRNA target and degrade the mRNA sequence, resulting in a decrease in luciferase activity...

...to target an ssRNA of interest, for example, a sequence specific to a virus or pathogen. Once Cas13 recognizes...recognizes and binds to the programmed sequence, it can promiscuously cleave surrounding ssRNA molecules...SHERLOCK. Cas12a is targeted to a specific DNA sequence, such as the Human papilloma virus (HPV) genome...

...amino acid sequence required for BirA recognition and biotinylation. This 13 amino acid sequence is termed...Biotin can be added to either terminus of a DNA sequence during a Polymerase Chain Reaction (PCR) by using...

... Both the plasmid (blue, backbone) and the DNA sequence of interest (green, insert) are cut with restriction...in the direction you want - usually by Sanger sequencing or further restriction digests. Of course there...appropriately sized insert, you should send it for sanger sequencing using primers that will allow you to read over...

... of DNA to create a synthetically designed DNA sequence. There are multiple methods of DNA assembly available...experimental and primer design based on fragment sequences and the polymerase used in the amplification. ...

... downstream methods, such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for...suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s the challenge in that?...

...eukaryote (S. cerevisiae) to have its genome fully sequenced There are several human disease models established...Genome manipulation K. phaffii’s genome has been sequenced for some time, clearing the greatest hurdle for...

...behavior, many many images of brain regions, or sequences of microbial DNA they all seem to require some...biological tools. In addition to the researcher using sequencing technologies to study microbiome interactions...

...used by scientists to introduce different DNA sequences of interest into a bacterial cell or a host’s ...direct contact. The donor bacterium contains a DNA sequence called the Fertility factor (F-factor). The F-...

...scaffold and cloning sites for inserting the guide sequence (pSpCas9(BB)). The 2A-GFP allows for screening... plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene’s pLKO.1...

...are necessary for recognition and cleavage. This sequence flexibility indicates that gRNAs could be incorporated...tRNA-gRNA and tRNA-gRNA-tRNA, with short 5’ spacer sequences preceding gRNAs, to test for gRNA cleavage. gRNAs...

...heterogeneous when constrained by sequence identities in flanking sequence (“microhomologies”). NHEJ doesn...

...libraries will require access to next generation sequencing technology. These libraries are lentiviral ... including a tool for getting optimized guide sequences for activating any human gene, go to http://sam.genome-engineering.org...enabling systematic investigation of the cellular consequences of repressing or inducing individual transcripts...

...PEDAR strategy to delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me...

...flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids for flexibility... flanking an RNA payload to insert the payload sequence into the genome. The team established that they...elements allowed for scarless insertion — no UTR sequence added to the genome (Figure 4). Figure ...