...Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs...for your research during this holiday season and we look forward to seeing what awesome research you and... offering to share their stories – about their research, their job hunts, and their tips for experiments...you have any questions or if you have a topic, researcher, or technology you would like us to feature in...looking for guest bloggers who want to share their research with the community, so email us at [email protected] Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking...

...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...safety of these vectors is still an active area of research due to the possibility that mutation or recombination...lentiviruses with human tropism can still infect the researcher and insert into her/his genome - this is a biosafety...

... large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection of BRET-based.... E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...

...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as HR... the first time in the history of neuroscience research we have a technology that allows us to show causality...discarded but clearly we are in an era of neuroscience research where optogenetics will be a standard tool used...the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA IRP), with the stated goal of...they can be used is to consult the many primary research papers that have been published at this point....through hydrogen ion efflux; evidence suggests that Arch may more effectively silence neurons than HR. Delivering...construct using stereotaxic surgery is a neuroscience research technique often used beyond just optogenetics....

...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics... technique in molecular biology and scientific research. Get an overview of these methods in this blog...fantastic year and happy reading. Best wishes on your research in 2020! Additional resources on the Addgene ...

...contributed by guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically... thanks to our guest bloggers Marcelle Tuttle and Alex Chavez! Marcelle Tuttle is a medical student at...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find... at Tufts University and former researcher at the Wyss Institute for Biologically Inspired Engineering...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

...  Easily search for plasmids (and more!) The main goal for the homepage was to improve search and browse...redesign, the new search bar is now front and center, with a clear call to action - Search! Along with this...It became clear to us that we needed to improve search and navigation, while also updating brand messaging...Community, sharing, and fun Along with improving search and navigation, we also wanted to emphasize the...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene'...website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...your favorite search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,”...have masters by using the search term “master’s degree.” Use the LinkedIn search filters to limit results...postings  Look at master’s-level job postings on a job search website like Indeed. Do the job descriptions sound...manuscript and grant preparation, and lead your own research project.  3. Do you prefer being a generalist?...variety of projects and in different fields of research is one of the best parts of my career with a master...master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized so ...

...in full-length publications. As early career researchers, we wondered how we could make the insights gained...around reading through entire papers, perhaps you search google images for specific experimental results...These data are valuable but remain hidden from research papers as they are not included in traditional...study with multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making... the NIH and Wellcome Trust now accept interim research products (pre-registration, preprints, data, software...micropublications) in grant proposals. ReFigures are one such research product which can convince reviewers of your expertise...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...mechanism of incorporation is not well understood, researchers have been using AAV vectors for gene editing ...iPSCs from ~0.2% to ~1.5%. In more recent work, researchers have combined AAVs with CRISPR systems. Ohmori...an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components...delivery method frees up space in the AAV allowing a researcher to fill it with the repair template. Bok and ...hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently deliver... continue to use and develop AAVs for your own research and encourage to deposit any new AAV tools you...Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...