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We narrowed to 928 results for: Gene

Showing: 131 - 140 of 928 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...aberrant activation of nearby genes. These safer vectors have become standard in gene therapy. Researchers have...condensed to create the 2nd-generation lentiviral system (Figure 2). The HIV genes that do remain are very...into the genome, they could promote oncogenesis by altering local gene expression. Self-inactivating (SIN... LM, Rebello T. J Gene Med. 2013 Feb;15(2):78-82. PubMed PMID: 23322669. 6. Genetic treatment of a molecular... Jr. Gene Ther. 2010 Feb;17(2):150-7. PubMed PMID: 19847206. Additional Resources on the Addgene Blog ...applications, and they’ve also had success in clinical gene therapy applications. Read on to learn more about...researchers are interested in developing these vectors for gene therapy. In 2003, VIRxSYS began the first clinical...

  2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...MSCV (Murine Stem Cell Virus) sequences. Packaging genes (viral Gag-Pol): Gag is a structural precursor protein... protein, and Pol is a polymerase. Envelope gene (may be pseudotyped to alter infectivity): The use of...both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol, and Env), the ...and/or Env, eliminating the need to deliver these genes in trans by specific plasmids. This method, therefore...standard γ-retroviruses use the Gag, Pol, and Env genes for packaging; however, they are different viruses...replication incompetent? Yes, as with lentiviruses, the genes required for γ-retroviral packaging and transduction...U3 region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...multiple gRNAs targeting a single gene can increase the likelihood of gene knockout, or multiple gRNAs may...genome editing has been used to study the effect of gene knockout in vivo and in vitro, as well as to insert...observe a phenotype, the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly... al constructed polycistronic glycine tRNA-gRNA genes (PTGs) in the forms tRNA-gRNA and tRNA-gRNA-tRNA...constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs...may be used to target genes with overlapping activity. Multiple gRNAs can also facilitate deletions of...viruses have illustrated the power of polycistronic gene organization, and its translation to CRISPR/Cas9...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... or “LSL” cassette) upstream of a gene of interest will prevent gene expression in the absence of Cre....Cre-dependent gene knockout – conversely, putting the loxP sites on either side of a gene (called “floxing... loxP”), will permit gene expression until Cre is present, at which time the gene will be disrupted or...most common uses are listed below. Cre-dependent gene expression – placing a series of transcriptional... presence of Cre, the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid...switch regions evidenced through Cre–loxP–mediated gene targeting. Gu, H., Zou, Y.R., and Rajewsky, K. 1993...reciprocal crossover event. This event can have three general outcomes based on the location and orientation ...

  5. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... (NHEJ) pathway disrupt target gene sequences (thus inactivating genes), while breaks repaired by homology...error-prone repair of the gene would be disastrous (i.e. if breaking the gene is worse than leaving the...(in our hands) this technique supports gene disruption or gene replacement frequencies that are an order...repair (HDR) pathways can modify the sequence of a gene (thus altering its function). HDR is crucial for...consistently supported higher frequencies (up to 60%!) of gene editing than single or double stranded donor DNA...editing, but it could be very useful to tackle genetic diseases in which sequence replacement holds some...PubMed Central PMCID: PMC4032847. Resources on the Addgene Blog Brush Up on Your HDR Basics Learn How to ...

  6. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... delete/overexpress endogenous genes or to introduce heterologous genes, but the trend of research has...tinkering with regulatory networks and multiplex gene control. However, these approaches are often met...mimic natural transcriptional programs on multiple genes. Modifying the CRISPRi system for multidirectional...programs to differentially alter the levels of five genes that encode biosynthetic enzymes for violacein, ...recruit various transcriptional regulators to alter gene expression at the targeted site. Multiple scaffold...simultaneous, multidirectional and tunable control of gene expression. This strategy can be applied to effectively...RNA-guided platform for sequence-specific control of gene expression. Cell, 152(5), 1173-1183. (2013). PubMed...

  7. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...profiles (Dwight, 2011).  Reference Genes  Choosing a set of reference genes deserves more attention than it...reference genes, plural. Your data will be much improved if you move to using multiple reference genes whenever...reference gene being a common choice - the frequently used GAPDH has noted issues as a reference gene (Higgins... Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming... it usually receives. Different reference genes have shown to have as much as sixfold variation in expression...), so you’ll want to ensure that your reference genes are suitable for your cell lines and/or tissue types..., and it's possible to multiplex your reference genes to save on plate space. For many cell lines and ...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...easily modified to tag hundreds of genes, generating powerful genetic screening libraries. Additional protein... to screen for the genetically modified strains that contain your newly tagged gene. Most insertions can... to generate high-throughput targeting vectors. These vectors can be used to tag endogenous genes and ...-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4) (2016):1277-88....protein fusions is to replace the genomic copy of your gene of interest with a fluorescent protein fusion. CRISPR...allowed scientists to tag and light up endogenous genes of interest to better understand normal protein ...easily create C-terminal tag fusions of endogenous genes in human cells. To use CRISPaint, users need 3 vectors...

  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...that of adjacent cellular genes. Q7: Which genes are deleted or modified to result in replication deficiency...including the gene of interest, and the plasmid coding for an envelope. Three generations of lentiviral...minimal number of HIV genes required for virus production (3 or 4). Third-generation vectors contain gag...gag, pol, and rev. Second-generation contains those three genes plus tat. The envelope plasmid provides...are useful and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses...lentiviruses and gamma-retroviruses use the same genes for packaging (that's gag, pol, and env), the isoforms...infect dividing cells. When choosing the appropriate gene delivery system, the specific target cell or tissue...

  10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...forward engineer a system of 10 genes. You can now forward engineer a 10-gene system to do something. We know...collection of genetic parts for precisely forward-engineering the central dogma across many genes simultaneously..., off-the-shelf genetic elements that allow you to begin to imagine designing 10-gene systems with quantitative...used over and over again in combination with novel genes to get very precise expression levels. The BIOFAB...otherwise invisible molecules into regulation of gene expression including growth or fluorescence. There...our testing of these parts that the chance your genes will be expressed at the level you want is 93 percent... of biological systems today who are building 10-gene systems from scratch. (They may be building genomes...
Showing: 131 - 140 of 928 results