...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...general intro until you know if the person you are talking to is really in your direct area.  Review the conference...see when your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn...colleague. Recommended Reading & More: Scientist Networking: What is an Informational Interview? Crash Course...Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships for Success" webinar... improving your networking and soft skills ... scientist networking...

...continued working for Palladian Partners remotely, but started looking for a new job because “it kinda sucked...social media manager. “I usually start out my days looking through our Twitter feeds and dealing with social...difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills that ...include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things quickly. Story telling...government, so it was a really good environment to be looking for a job.” Thanks to Susan for not only being ...

...Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current... responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences...take some research.  It helps to spend some time thinking about what your goals are for the coaching relationship...out of academia (because so many scientists are making this transition). Another coaching group is Still...Humphrey from Jobs on Toast is based in the United Kingdom and scientists there would be lucky to work with...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. I...Entropia Consulting works remotely, will travel for speaking engagements and has many areas of specialty from...

...with different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast) ...bones and teeth. It is an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator...sensor to use for your studies, you can start by asking ‘What is the biological phenomena I want to observe... a sensor that is tuned to your parameters.   Kinetics How fast is the process you would like to monitor...jGCaMP7 and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...to variance in sensor expression if you aren’t working with a stably integrated biosensor. Ratiometric...

...ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand is also important for ...delayed and takes on the order of minutes. Studies looking at immediate effects of specific neuron firing ...blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies is whether or ... are viable for translation to human patients. Picking receptors The receptor you choose is largely dependent... you to directly manipulate ion channels. When working with PSAM receptors, your impact on the cell is...Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-...

...pretty good about sticking to that. If you email us at noon and then call back at 1PM asking why you haven...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy...doctoral dissertations. Streamline the question asking process Having thought through the above, don’t...soon as possible!” and I always begin my responses asking for more specifics. We can help you faster if we...how long it will take to get an answer, consider asking to avoid unrealistic expectations. And finally... Eric J. Perkins...

...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion...light is more phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over...from a dark state to a bright fluorescent state (“kindling”), and from a bright state to a dark non-fluorescent...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying...PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion of the monomeric red fluorescent protein...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

...governing policies are developed primarily by wishful thinking. The March for Science is about fighting scientific...postdoctoral researcher at Harvard Medical School working on human artificial chromosomes. Montreal, QC, ...scientists, we work tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how ... I was scared. I worried that, because we were speaking out against legislators and because the march ...AggleDetector on Twitter) is a graduate student at Johns Hopkins University, where he is trying turn a few exoplanets...it takes to complete your resume, without ever thinking that research scientist would be a career that...NIH budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught ...

...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell cycle. Good...different chromosomes. The advantage of having this kind of system that works in live cells is that one can...used with your own reporters to decipher their trafficking routes. This collection is now available at Addgene...indicators should possess fast voltage sensing kinetics and have no spectral overlap with the optogenetic...and an endoplasmic reticulum export motif and trafficking sequence for improved targeting to the plasma...CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator, QuasAr...

...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...make when designing a western blot.  Psst! Looking for a western blot protocol? Check out Addgene'...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...re running native (non-reduced) proteins or are looking to preserve specific protein modifications, we ...single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the same size, or a slightly...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...