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Showing: 131 - 140 of 640 results
  1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    Published
    Nov. 12, 2019, 2 p.m.
    ...process samples at the picnic table before dark, a bison wandered into our campsite. We had to abandon our...our samples and move farther away until the bison meandered off. In the end we were able to safely get...get back to our lab work. Figure 3: When a bison wanders into the campsite, leave your samples behind...ThermoCas9 from the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9...
  2. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...   In fact, there are a lot of good reasons to stay and good reasons to go.  Here are some questions and...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change...
  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...Although CRISPR/Cas9 is all the rage, they had another reason to test this system. It’s difficult to determine...require multiple transduction events. For these reasons, iTOP is best suited to binary systems in which... size associated with viral vectors. For these reasons, iTOP, especially in tandem with genome editing... VP22 nuclear homing an artifact? Lundberg M, Johansson M. Nat Biotechnol. 2001 Aug;19(8):713-4. PubMed...
  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ... T. M., Edwards, A. M., Healy, L. M., Robertson, J., & McPherson, P. S. (2019). Implementation of an antibody...perform as well in other conditions. For these reasons, it is essential to test the selectivity of your...cannot prove antibody selectivity, and for that reason, it is best practice to combine correlation with...C., Bandrowski, A., Virk, H., Edwards, A. M., McPherson, P., & Laflamme, C. (2023). Scaling of an antibody...
  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the... by their focus on specific organisms. For this reason I generally recommend that laboratories that have...CRISPR technology to a new organism: For obvious reasons you can rule out any tool that limits you to a ...
  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...make for an excellent model organism for a few reasons: Affordable to culture and easy to work with ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...aren’t going anywhere anytime soon, and for good reason! However, there are other players in the game (...
  7. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ... by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University...in monoallelic diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that...fibrosis (8).   The difference with CRISPR/Cas9 in comparison to other genome editing techniques is its ease...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...
  8. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Published
    July 27, 2017, 1:11 p.m.
    ...fluorescent proteins everyday.  The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...Plasmids from the Tsien Lab Browse the Michael Davidson Collection ...
  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor...depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However, determining this ...to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty ...page: https://ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy...
  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...degeneration; Laurenz Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking...clinical trials for ALS.   I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ... are key to the research, but they are also the reason this research is happening. Stem cells scientists...
Showing: 131 - 140 of 640 results