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TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....fluorescent proteins everyday. The late Michael Davidson and Roger Tsien played enormous roles in enabling...their legacy, we recently launched the Michael Davidson and Roger Tsien Commemorative Travel Awards. These... we’re proud to announce our first two Michael Davidson and Roger Tsien Commemorative Travel Awardees:...Plasmids from the Tsien Lab Browse the Michael Davidson Collection ...
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Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School of Management. Professor...depositing a plasmid with Addgene, in contrast to other reasons for the correlation. However, determining this ...to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies Pamela McA'Nulty ...page: https://ssrn.com/author=1232466. Neil Thompson is an Assistant Professor of Innovation and Strategy... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....make for an excellent model organism for a few reasons: Affordable to culture and easy to work with ...established in yeast, including a widely used Parkinson’s experimental model. In fact, approximately 25%...of the uses outlined below. Biology They say comparison is the thief of joy, but I think it’s acceptable...aren’t going anywhere anytime soon, and for good reason! However, there are other players in the game (... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...degeneration; Laurenz Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking...clinical trials for ALS. I have an uncle with Parkinson's, a half-brother who is a quadriplegic due to ... are key to the research, but they are also the reason this research is happening. Stem cells scientists... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog PostPublishedSept. 8, 2015, 2:30 p.m....Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte...Chemical effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....come into being through the actions of a single person but through the coordinated efforts of many individuals...collection page, we certainly don't expect that one person will write all the copy, design every icon, curate...page. That would be entirely too much work for one person, it would take forever, and the end product would...who can do the job. It is good to delegate so the person doing the task will have to learn and stretch a... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....in the form of a protocol, and you can add in personalized steps like “check drawers for light-sensitive.... References and Resources Images used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7... 7, 2022. https://www.med.upenn.edu/robertsonlab/assets/user-content/documents/Western%20Blotting.pdf ... Rachel Leeson... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization..., and ColE2 plasmids) as cell division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that...References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher A. Voigt. "Engineered promoters... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....AAV pRDA_889 (ITRs from AAV2) Mammalian Transposon pRDA_757 (Piggybac; constitutive expression... drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector (dropdown options..., Bosch, J. A., Zirin, J. D., Viswanatha, R., Sontheimer, E. J., Goodale, A., Greene, M. A., Green, T.... Rachel Leeson... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m.... What is this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described...has led to the alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation...Müller, Sara M., et al. "Quantification of Förster resonance energy transfer by monitoring sensitized emission... Jason Niehaus...