We narrowed to 432 results for: sequence

...regulatory sequences to allow for expression of multiple gRNAs (i.e. Csy4 cleavage sequence, tRNAs, multiple...No dsDNA cleavage Blunt end 5' overhang PAM sequence NGG TTTV PAM site preserved? Usually Destroyed...multiple promoters to drive crRNA expression and sequences that allow for array processing, i.e. tRNAs or...Lentiviruses carry a (+) strand RNA copy of the DNA sequence, which is a suitable substrate for Cpf1. This ...

...side of the fluorescent protein sequence that is homologous to the sequence into which it is being inserted...Gene editing is the addition of a useful coding sequence (i.e. GFP) into a gene while leaving other features...in one edited allele, or gene copy. The coding sequence for GFP is transcribed and translated along with...the gene of interest, a linker, and the GFP tag sequence to be inserted. Created with BioRender.com. ...

...adapted the native PEG10 RNA sequences to instead drive packaging of cargo sequences in the capsid, in an approach...also optimized the architecture and amino acid sequence of the prime editor, yielding PEmax, to work synergistically...plasmids  Deletion and replacement of long genomic sequences The PEDAR strategy engineered by Wen Xue’s lab...double-strand breaks and deletes the intervening sequence. As with other prime editing tools, the desired...

...gRNA targeting sequences, you can then readily generate your barcoded gRNA library sequences via oligo synthesis...can be tracked and quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible...will need is to get the list of effective gRNA sequences targeting your genes of interest. Thanks to the...where NNN and XXX represent the 20-bp gRNA target sequence and its 8-bp barcode, respectively. Once cloned...

...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be... Description Origin of Replication (ORI) DNA sequence which allows initiation of replication within ... be exploited for sequence verification of plasmids.   How is a plasmid constructed in the lab? Due...

...described in this post are engineered to bind RNA sequences derived from Zika virus and, upon binding, produce...stripped down viral genomes containing only the sequences required for viral replication and transcription...that can be used to quickly barcode a set of DNA sequences. In this post, Alan Wong (formerly of the Lu Lab... variety of customizable functions to your DNA sequence of interest. Potential applications of the system...

...cloning), adaptor ligation for next generation sequencing (NGS) library construction, and circularization...These applications detect specific nucleotide sequences with high specificity and quantitative accuracy...Detection Reaction (LDR), probes complementary to the sequence of interest are annealed to target DNA (genomic...fidelity thermostable DNA ligase. If the target sequence is present, the probes will ligate. Subsequent... contacts, and thus do not read specific base sequences, but are sensitive to distortions in helix shape...Gelfand. "Cloning, overexpression and nucleotide sequence of a thermostable DNA ligase-encoding gene." Gene...1756968. 7. Lauer, G., et al. "Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus...

...injected animal.  PAM sequence considerations For changes 5’ to the PAM, sequences corresponding to the...developmental processes, CRISPR/Cas9 allows us to delete sequences and introduce mutations and epitopes with unprecedented... but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated excision of the...the selective cassette minimizes the sequence added, leaving a 34 bp “scar”, but currently requires additional...editing efficiency, while for edits 3’ to the PAM sequences corresponding to the opposite strand were better...they were able to insert 10 kb of non-homologous sequence or a series of single-nucleotide substitutions...an alternative gene editing system for AT-rich sequences https://doi.org/10.17912/W2237D Farboud B, Severson...

...the need for genome sequencing or annotation (5). In the event that genome sequencing can be done, this ...problem: Host binding proteins are hard to find Sequencing and cloning phage DNA can be difficult, in part...(4). To further complicate matters, even when sequenced, phage genomes tend to be choc-full of open reading...technique can also help pinpoint the genes within a sequenced genome that encode for functional cell-binding... a restriction enzyme with a short recognition sequence (like MluCI) that will cleave DNA frequently enough...Now you can grow up your colonies of interest, sequence the inserted DNA and characterize your new putative...

...insertable sequences or specific mutations, along with homologous DNA to the target sequence to be modified...and deletions along the way. HDR uses homologous sequence as a template for repair and is thus the more ...used for repair is identical to the original DNA sequence at the DSB, or it can introduce specific mutations...non-crossover events. This means all newly synthesized sequences are retained by the original damaged DNA molecule...is introduced disrupting one or both of these sequences. To prevent re-cutting and the resulting mutations...