...New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the... We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were videos...guiding completely new members of the lab or those taking introductory lab courses. The Intro to the Lab...science techniques. It’s also great for teachers looking to supplement their curriculum. From the details...

...the specific Rho GTPase/GEF pair (Ridley, 2016). Taking control of a RhoGEF is a great way to stimulate..., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens of family...Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from ...chemical composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically....2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution...

...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...antibody is extremely rare. While many function-blocking integrin antibodies exist, almost all bind outside...Springer lab, reported the binding specificity, kinetics, and affinity of these 11 newly discovered synthetic...to the Springer lab, a highly specific function-blocking antibody, αVβ3.13, may also block function of ...important integrin on these cells. What’s next IPI is working with Addgene to accelerate biomedical research ...

...protein required for cytokinesis in JEI. Developing an interested in biology, working in a lab for the first...(the importance of word of mouth and personal networking!) and the manuscript review/submission process... first time, and thinking about the future. Many thanks to our guests,Olivia Ho-Shing, Mark Springel,...

...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...GIFs? We have answers! via GIPHY   We’ve been working hard to create the most informative and entertaining...protocol videos. via GIPHY   If you’re also looking for that perfect GIF for your TikTok or Instagram...us know on Twitter @addgene! Addgene is always looking for new ways to help you celebrate those wins in...

...If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options —... — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody...antibody catalogs at the same time.  If you're looking for an antibody, especially a validated antibody...antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn...

...always gets done this time of year 😁 we stopped working on our reports/lab stuff for a few minutes to create...ladies #research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats...lab went all out to transform their lab into a walking tour of Buddy the Elf’s journey from the North ...fireplace scene.  Got our @EmoryChem lab office looking festive 🎄🌟🎁 @Addgene #DeckTheLab pic.twitter.com...

...material so that you can more easily find what you’re looking for. Whether you’re joining us for the first time...the blog that will give you a better experience. Looking for something? Use the navigation menu. The new... the top of the screen and type in what you’re looking for. After you search, you’ll see the blog articles...bloggers Like a blog post from a certain author and looking for more from them? Check out the new author pages...

...Seeding Labs Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute... health. 40:17 - 44:14 : Microbiome research - working with bacterial communities both on the human body...microbiome expertise. Many thanks to Kwabena Duedu for taking the time to sit down and talk with us.  Kwabena...the Addgene, Seeding Labs Plasmid grant and is working on a variety of projects in Ghana.     Special ...

...competent lentiviruses (RCL) especially if you are working on HIV positive human cell from the clinic. The...extra care has to be taken especially if you are working with proto-oncogenes or with human samples that... is now used as it shows rapid gene expression kinetics as compared to the Tet-off system. One drawback...one with either tTA or rtTA (FUW-M2rtTA). In this kind of approach, a population of cells might express...transgene resulting in low inducibility and potentially making it difficult to interpret your results. This can...

...a startup and back to academia. Chapter 1: How working at a startup prepared Tom for an academic career...more complicated biological devices. Chapter 6: - Making research go faster through Open Access. Chapter...investigate synthetic biology at Boston University, working in one of the founding groups of the field under... Plasmids 101: Golden Gate Cloning Learn About Working at a SynBio Startup in Our Interview with Michael...

...when did you start thinking about them? 18:30 - 19:54 : How to think about picking a post-doc project ... you leverage your community, think hard about picking a good mentor, and begin considering career options...Mentoring" We'd like to thank Niroshi and Ben for taking the time to sit down and talk to us and wish them...

... including that it’s never too early to start networking! Learning about careers at Addgene Afterwards...popular, with the students excited to see a real working lab full of busy Addgenies preparing plasmid samples...impressed our guests, as did how much work is put into making sure scientists all over the world know that we...Advice from Our Bloggers Learn How to Improve Your Networking Skills  ...

...providing quality control and customer support, making plasmids easy for you to find and request in our... Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual plasmids ...Stabs are organized by shipment and moved to our packing area.Using the barcode system, the correct plasmids...necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence, answer scientists...

...Brownstein, an Addgene board member. When I was working in Human Resources at Harvard Medical School, Joanna...Joanna was my colleague. She mentioned Addgene was looking for someone with HR expertise to join their newly... her forever home. I feel blessed that I’m not working so that I’m available to spend time caring for ...for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening. Learn more about...

...expression cassettes (Shaw et al., 2023). Those looking to express high levels of their gene of interest...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was designed to work in....2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports...

... don’t want to spend months doing surgeries and making fiber optic cables only to hook up your lasers ...alter the light spectrum for activation and the kinetics of opsin activity/inactivity. Dr. Deisseroth has...(not expressing the opsin) are also available. Picking your opsin  You must consider whether you wish ...neuronal firing, the cell-type specificity, the kinetics of the opsin variant, and other particularities...the promoter of the cre recombinase while still taking advantage of the robust expression via the promoter...

...Tools for Making Scientific Graphics by Beth Kenkel Find a round-up of tools you can use for making scientific...we're gearing up to start a new decade, we’re also looking back at the last year of scientific discovery. ...Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by Joachim Goedhart Guest...

...Francisco International Airport listening to the Lion King soundtrack and writing this post, it is my pleasure...what new post contributed the most to this record breaking month and to find other posts that deserve a second...Navigate a Scientific Enterprise in Flux CRISPR Kinome Libraries Available: Pooled and Individual Plasmid... Research Community Grad School Advice Part 1: Picking a Lab and a Project That’s it for now. As I indicated...

...at a lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed... in November 2017. #Phage researchers! I am working with a team to get Burkholderia cepacia phages ...must recognize a specific part on the bacterium making each phage is very specific. Some infect only one...about scientific sharing Find blog posts about networking Additional resources on Addgene.org Find molecular...