We narrowed to 890 results for: IND

...other important binding events take place during the course of infection. These binding events can be impaired...infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult...possible. One problem that often plagues labs and hinders experiments is mycoplasma contamination. Mycoplasma...Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research Brush Up on All ...

...nose-poke at a particular hole (the active hole) induces laser stimulation of the chosen brain circuit, .... If activation of the circuit is sufficient to induce reinforcement (instills a sense of “reward”), then...software such as EthoVision. Consult the literature to find all the applications of optogenetics relevant to...region may affect the animal; stimulation could induce seizures or some other unwanted phenomenon or it...learn optogenetics is straight through the source - find a lab within your own neuroscience sub-discipline...

...hero (that I’m not), FRET loves playing “live.” Indeed, FRET was one of the first techniques which enabled...Historically, molecular interactions were detected by indirect means often using probes with the potential to...may have to design several different constructs to find one that could be used for the purpose of your studies.... But it is a parameter that you have to keep in mind when analysing FRET results. The cross-talk between...also don’t have any resonance transfer. You have to find a balance between FRET efficiency and cross-talk...

...University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting...efficiency gene targeting approaches improved on this finding with the addition of selectable markers that are... et al 2004, Kim et al 2008). While the biology behind this AAV effect remains unclear, it is likely not...infect recipient cells. After G418 selection, individual gene edited clones are identified by PCR. Then...Resources on Addgene.org Check out our Viral Service Find AAV Vectors Browse all Viral Vectors ...

... Joanne's 10 Things Scientists Ask about Finding an Industry Job Talk What’s Your Story? By Herminia Ibarra...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview...before tailoring each presentation appropriately.  Find out who will be listening.  Are they early, mid,...explanation. The people that need the information will find it. Experienced speakers present useful protocols...

... of E. coli used today are descended from two individual isolates, the K-12 strain and the B strain. K...used in the lab Most of the commercial strains you find today are marketed for a specific purpose: fast ...conjugation. Genes listed on F´ are wild-type unless indicated otherwise fhuA (formerly tonA) ferric hydroxamate...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...

...’t quite seem to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody...very new to antibodies as research tools, you may find “Chapter 1: What is an Antibody” quite useful. If...place to start looking for answers.    We hope you find the latest addition to our eBook collection useful...

...Addgene.org Check out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian ...including one loxP site insertion and one NHEJ-repaired indel, often occur. Figure 1: In conventional gene...recombinases (Cre, Flp etc.), and tetracycline drug induction systems (ttA or rtTA) are between 1 to 2 kb in...al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with most projects...

...Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular...Chapter 8 - The importance of communicating research findings through social media and other nontraditional ...

...Science rap mastermind, Tom McFadden, recently worked with high school students in the bay area to create...listen to the interview with Tom McFadden!   1. Rosalind Franklin vs. Watson & Crick - Science History ...story telling following the unfortunate story of Rosalind Franklin and her incredibly important role in ... Student Community Check out All of Our Podcasts Find Great Educational Content in our Plasmids 101 Series...

...red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you...nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats Many teams recycled lab...ladiesinlabcoats #Christmas #labchristmas #cute #reindeer #DeckTheLab #eppendorf #themuppets #scifun #funny...

...Researchers in developing countries often find themselves on the front lines of emerging global challenges...and Africa, but we need to expand our efforts to find new and creative ways to reach developing countries..., as well as shine a light on some of the great minds working in developing countries. As the world continues...and organizations like it. Additional Resources Find out how you can get involved with Seeding Labs Follow...

...widely used class of protein-binding reagents. In the lab, their binding specificity allows scientists.... The ends of the branches contain the antigen binding sites and are highly variable, allowing exquisite...The immortalized cells, called hybridomas, are individually isolated and expanded. The clonal cells are ...

...expression of Red include the IPTG-inducible lac promoter, the arabinose-inducible pBAD promoter and the endogenous...direct modification of DNA within E. coli and is independent of restriction sites. The lambda red system is...with a higher efficiency than the other and you’ll find out empirically which is the lagging strand. ssDNA...Resources on Addgene.org Browse our Microbiology Pages  Find Lambda Red Plasmids and Strains Check Out Other ...

...calcium binding protein) fused to two fluorescent proteins. When the responsive protein binds to calcium...Venus fluorescent protein, the Troponin C Ca++ binding domain, and NanoLuc® Luciferase. CalFlux VNT responds...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...Plasmids Browse the Fluorescent Protein Collection Find Fluorescent Protein Biosensors ...

...in a variety of cell types, but you should try to find the best AAV for your particular experiment! Traveling...previous literature using AAVs for DREADD delivery indicates ~7-21 days are required for the onset of gene ...neural tracing in very specific cell populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) ...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001):...Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use AAV...

... used during the purification procedure. Good indicators of DNA purity are the absorbance measurements...nm should fall ~1.8 - 2 and give scientists an indication of purity versus protein contaminants. The 260...nm ratios should fall ~2 - 2.2, and provide an indication of purity versus chaotropic agents as guanidine...Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

... down What happens if you do scroll down? You’ll find Blugene, as well as information about Addgene, our...our three most recent blog posts. Finally, you’ll find everyone’s favorite section: Tools of the Day. Our... obvious ones to discover as you browse. You may find one of your most frequently used buttons stands ...faster to skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to ...

...Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...

...) suggestions for defining the problem and for finding solutions. Even better than just reading this article...Berkeley and Harvard. Students also partner with independent career organizations to get the grants and programs...There are many academic administrators who are finding ways to provide new types of career preparedness...years. However, some organizations are woefully behind and some just don't have the resources to do enough...

...could be managing a team, planning meetings, and finding the best way to organize your time. At a conference...social media, you could be sharing your research or finding opportunities to bring science to the community...you love, you have to create opportunities. If you find yourself asking, "Why doesn't this product/organization...transferable skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight ...

...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...group to get real feedback Write whenever you can, find supportive editors so your first drafts are always...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the .... For More Reading: From Academic Solos to Industrial Symphonies by Gwen Acton, Alicia Gómez-Yafal ...

...In that time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions...institutions. This has given us a unique window into technology trends in the life sciences. In this post, we'll...two most popular plasmids of the past 10 years. Induced Pluripotent Stem (iPS) Cells - 2008 was marked ... GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year in our collection...

...The results are light-inducible transcriptional effectors (LITEs) designed to bind specific genes and turn...Zhang’s lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...methyltetrahydrofolate, which are universally abundant. Second, induction of transcription occurs within minutes of light...

...Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization Find Fluorescent Protein Empty Vectors...jump from one antigen presenting cell to another. Indeed, we now know that T cells are able to crawl more...proteins (proteins that fluoresce after a light-induced chemical reaction) or photoconvertible Keade proteins...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to...

...mechanism. The Cas13 effector binds the guide RNA (shown as mature crRNA) and binds the RNA target before initiating...often recruits the main effector complex after binding to a target. Class 1 is the most abundant of the... functions as both a helicase and a nuclease, unwinding and cutting up DNA after recruitment by Cascade... large-scale clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is...

.... Resources at Addgene Find optogenetics plasmids! Find AAV resources! Find fluorescent protein resources...the practicalities and considerations you may not find in the materials and methods sections of optogenetics...Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nature neuroscience. ...

...determine if there have been any early successes, and to find out what delivery techniques researchers are using...posts throughout the month including a first of its kind for the Addgene blog - Addgene Software Engineer...programming noob (like myself) can understand. You’ll also find actionable advice on how to give a great science... (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order here...

...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which particular...Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled Libraries for Your Research...

...Violet-excited GFP (vex) Rosa26-Cas9 I-CHIME Inducible knockout of a single gene pXPR_068 Violet-...immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-CHIME and ...preparations than the first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral... A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential knockout of genes...

...posts Resources on Addgene.org Find plasmids for your research Find cancer research plasmids and resources...amplifications and fusions, missense mutations, and indels (Ng et al., 2018). From this data, they generated...The researchers used the collection to screen individual perturbations in vitro using Ba/F3 and MCF10A...

...plant, Drosophila and mammalian cells, and it may find wide use in biological research. Photoconvertible...color was observed after excitation at 488 nm, indicating that spontaneous conversion did not occur. Tobacco...protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication at Addgene. Subach...31;8(9):771-7. doi: 10.1038/nmeth.1664. PubMed. Find plasmids from this publication at Addgene.   Addgene...

...out a career path? Many trainees are developing Individual Development Plans (IDP's) through Science Careers...easy to miss out on beneficial opportunities or to find oneself unsure of the next steps. Harvard PI Angela... look the same in every lab, but the principles behind this technique are beneficial for researchers at...   References 1. Yearly Planning Meetings: Individualized Development Plans Aren't Just More Paperwork...

... Below Bauer and Canver discuss the motivations behind this research.   Using CRISPR/Cas9 for targeted...deletion, the nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the...from the JOVE article here on Addgene's website and find more CRISPR resources.    Thank you to our guest... mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 nuclease system in mammalian...

...about how I could fill this time. I was relieved to find out that the two hours were split up between several... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...at Addgene. This training prepared me for the independent work on the QC team. I was responsible for helping...Addgene that means that it is possible to work the remainder of the three hours (outside of the core hours)...

...Understanding the biology behind brain development, learning, and behavior poses many challenges in neuroscience.... During transduction, the viral capsid proteins bind to the target cell’s surface proteins and this interaction... the AAV serotype used for a specific cell type. Find some transduction characteristics of select AAV ...our AAV guide Learn more about our viral service Find retrograde AAVs at Addgene ...

...future in science (see my previous blog on this).  Indeed, that was a primary message delivered by many of... tackle the issues with rules and practices of indirect costs.  The peer review and grant awards systems...annual NIH progress reports information on the Individual Development Plans (IDPs) for the graduate students...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

...recommend avoiding a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self...cellular debris look very similar. However, keep in mind that kit components (i.e. buffers, columns, or beads...columns or magnetic beads. Both approaches involve binding the plasmid DNA from your cleared lysate to a substrate...specifically designed with environmental sustainability in mind (Table 2). Table 2: Sustainability features highlighted...instruction manual will help explain the rationale behind each step and provide troubleshooting advice. In...

...Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles of interest...who knows where), now is as good a time as ever to find out the true identity of the line? Once you’re run...Contributing to Cellosaurus Generated a new cell? Or finding errors in the available information about a cell...over globe, with some surprising, and important, findings being shared. For instance, recently Cellosaurus...

...the Molecular Biology Reference Find protocols on plasmid cloning Find plasmids for your research ...temperature for a short period, and there are indications that Tris buffer is better than water in these...1874070701307010010 Additional resources on the Addgene blog Find five ways to purify DNA without a kit Read our blog...