We narrowed to 640 results for: Son

...population cluster would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity...

...monitor with biosensors? You can keep tabs on my personal favorite (the caffeine of the cell!)—ATP. There...

...  Rachel Leeson...

...Haery in 2017 and significantly updated in 2023 by Jason Nasse.  References 1. Tervo, D. Gowanlock R., et...

...electroporation of a human, for example, isn’t a reasonable option. Since viruses used in labs are derived...

... is based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla...

...in the graphic below - including some simple comparisons. The easy multiplexing afforded by PTGs will...

...Repression and Activation. Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...

...purification without a kit that outlined several reasons why doing something without a kit has advantages...

...  Rachel Leeson...

...will still recognize your protein. For the same reason, they’re also useful in experiments where the availability...

...you are going to adopt (i.e. restriction cloning, Gibson Assembly, Golden Gate etc.), and try to find the...

...validated their platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect...

... the brain.  References Bedbrook CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine...

...series of plasmids to study PPI using Förster Resonance Energy Transfer (FRET, Heckler et al., 2015) or...

...fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable Fluorescent Proteins...

...is a genome engineering powerhouse, and for good reason. It is guided by an RNA molecule, the gRNA, to ...

...scientific communication needs. The most often-cited reasons researchers might not post a preprint are reluctance...

...10.1038/nprot.2007.267 Stoeckius M, Hafemeister C, Stephenson W, et al (2017) Simultaneous epitope and transcriptome...

...loci Building on the work of Chen et al., Thoru Pederson’s lab used CRISPR to label multiple loci in distinct...

...the market between 1997 and 2000. There are many reasons why drugs fail - sex is one of them. Why might ...

...off-target sites from being cut. Slaymaker et al reasoned that, if they decreased the positive charge in...

...career counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research...

...to see if it leads you to the same conclusion. Personally, I like papers that can end with some sort of...

...within E. coli host cells. Image from Nielson and Voigt (2014).   We talked with Voigt about...

...recombinase of bacteriophage P1. Sauer, B. and Henderson, N. 1988. PubMed PMID: 2839833. PubMed Central...

...writer a chance to delegate funds to supplies, personnel, equipment, travel, etc. As a first time budget...

...interviewer asks you a smart question. There’s no reason you can’t then ask that same question in your next...

...and really get it.” On the other hand, there are reasons why grad school might not be the right choice. ...

...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...

...Facebook or Instagram. Key features Setting up a personalized feed is simple. You can enter as many search...

...behavior. A fact to consider: Rodents can hear ultrasonic frequencies (> 20 kHz, produced from fluorescent...

...Though rarer, you can find MoClo that relies on Gibson and other non-Golden Gate cloning methods. MoClo...

...disease and cultured cells. For example, in 2013, Jinsong Li and colleagues chose to explore CRISPR’s therapeutic...

...difficult to functionally manipulate and study in comparison to protein-coding genes (Rinn and Chang 2012)...

... in the lab but also causes outbreaks of food poisoning, the difference is virulence plasmids! This category...

...susceptible to artifacts and pH interference, and comparison between experiments can be more difficult due...

...performing these experiments have a good baseline for comparison. Performing WISH using these plasmids can result...

...D.A., Shoemaker, D.D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bnangham, R., Benito, R., Boeke...

...properties and can be used in quenching, FRET (Förster resonance energy transfer), and FLIM (fluorescence lifetime...