We narrowed to 812 results for: cha;

...localized, and how its localization and expression may change across various conditions. However, this system...Improved brightness and signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified...

...model can be generated, and drugs screened for a change in pathology. If the disease pathology is complex...Target identification Not all diseases are fully characterized or have a simple pathology. In these cases, ...

... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...used for negative selection on 5% sucrose plates. Exchange of the sacB gene for the gene of interest allows...

...growth is possible even if your job title doesn’t change. Master’s level training can prepare you to present... career with a master’s. While it's possible to change fields of research with a PhD, a master’s is less...

...Introducing changes and/or short peptides sequences into these variable loops is one method for changing a capsid...

...cells, but they are susceptible to cells’ defense mechanisms. The use of non-viral vectors, like transposons...sequences and then testing various combinations of changes. The basic functional version of the Sleeping Beauty...

...people like a good story and science involves characters – in this case you – on a quest to ask and answer...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

... apart a piece that can address a topic in a mechanistic way using the technology available.” Choosing...combining “new discoveries or technologies increase chances that your project will be fresh as well.” Don't...

...LOV2 and Zdk1 to dissociate due to conformational changes, and as a consequence, EB1 protein function is ...expansion of the genome editing toolkit. However, a challenge in optimizing base editing technologies across...

...antibody fragments typically engineered from heavy-chain only antibodies found in camelids. They possess ...H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These biotin-labeled...

...more. Downloadable materials are available free-of-charge from the ABRC Outreach website. Seeds, which are...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis...

...anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New... New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4...

...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is...

...particles containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B,...Redon R, Moullier P, Léger A (2015) Advanced Characterization of DNA Molecules in rAAV Vector Preparations...

...our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV tools, we...caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/labels, ...

...level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity...

... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful...

...the success of the change in expression of GFP, for the stop codon should have changed from “TGA” to “TGG...

...which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed that in Stbl3 bacterial.... https://doi.org/10.1007/s13205-012-0070-8   Chakiath CS, Esposito D (2007) Improved recombinational...

...general comments and adjust them if my opinion changed after this more detailed look.        Encourage... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression...

...antigen-binding fragments (Fabs) (Porter 1959) and single-chain variable fragments (scFvs) (Inbar et al., 1972) ...within the variable portions of heavy and light chains. Proceedings of the National Academy of Sciences...

...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...

...was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of...

... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer...

...Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA...extract DNA, sequence it, and analyze the data to characterize the microbiomes of the different samples. The...

...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

...scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...

...scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to...want to consider for future work placements. The chance to learn and collaborate all while experiencing...

...+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative...

...production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have...

...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (...

...and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically...gotten these services up and running. Both Alex Chavez and John Doench are members of the Addgene Advisory...

...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address...

...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...

...this into the agenda ensures that everyone gets a chance to talk and less vocal team members still have ...meeting a day in advance. This gives everyone a chance to review the agenda and add anything that is missing...

...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite...them in your experiments. Use the following flow chart to get you thinking about the types of experiments...

...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...

...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone...

...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...