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Addgene
Showing: 521 - 560 of 792 results

  1. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...anecdotal forms of information — identifying with a character, building emotional investment and following cause-and-effect... to date, invite people to ask questions, be approachable. Engage with your audience and try to learn ...

  2. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...and retract over many microns in response to pH changes without the need for ATP or other chemical fuel... of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab...

  6. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...involve DNA incorporation into the cell, but the mechanisms vary. Why transduce? So why use a virus to deliver...transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into a cell. The twist is...

  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...level of independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci ...An audience can be defined by any number of characteristics. Common ones include age range, familiarity...

  8. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... positive plants, but screening these can be challenging and requires specialized equipment. Reporters...Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy and beautiful...

  11. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... via an online process, though the process has changed a lot over the last twenty years! This has helped...UBMTA link for the recipient organization upon purchase. We recognize exceptionally fast tech transfer...

  12. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...production phase. However, this has not been well characterized as it is not a common event. What are exosome...2014), but scientists have yet to decipher the mechanistic aspects behind these improvements. What have...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...was very contemporary and futuristic with block chairs in the hallway for people to take a break and relax...our own space. Workshops such as this give us a chance to learn something new without spending a lot of...

  14. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA...extract DNA, sequence it, and analyze the data to characterize the microbiomes of the different samples. The...

  15. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

  16. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...while in Grad School Listen to Biotech Founder Michael Koeris' Journey from Grad Student to Entrepreneur...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...and proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically...gotten these services up and running. Both Alex Chavez and John Doench are members of the Addgene Advisory...

  18. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...iPSCs Symposium earlier this year, I jumped at the chance. Here was an opportunity to see Shinya Yamanaka...field have described the Yamanaka paper as "life changing". Dr. Yamanaka himself, during his keynote address...

  19. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...to write your colleagues’ names in a polypeptide chain, except if their names have a B, J, X, or Z. Some...

  20. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to...want to consider for future work placements. The chance to learn and collaborate all while experiencing...

  21. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...+ and Chloride (Cl-) ions. When the negatively charged iodine is added, it interacts with the CV+, creating...used in, and how components of the plasmid should change based on bacteria type. The gram positive and negative...

  22. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...side note, my guess is that people will not be exchanging business cards for awhile or ever so your LinkedIn.... If you really got along well, suggest a coffee chat in the near future. Even just one more meeting (...

  23. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...this into the agenda ensures that everyone gets a chance to talk and less vocal team members still have ...meeting a day in advance. This gives everyone a chance to review the agenda and add anything that is missing...

  24. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...target binding, Cas9 undergoes a conformational change that positions the nuclease domains to cleave opposite...them in your experiments. Use the following flow chart to get you thinking about the types of experiments...

  25. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...spatially limited studies and can fluctuate with changing culture conditions. The use of low intensity light...References Refactoring and optimization of light-switchable Escherichia coli two-component systems. Schmidl...

  26. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...with progress reports, meeting minutes and plan changes. Written documentation helps make sure everyone...

  27. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...the current members to share short-term goals and chat about awesome science. Important factors in the ...is the lab? While larger labs tend to be more "chaotic" and offer less micromanaging, they typically offer...

  29. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...original publication but its name was subsequently changed to clarify that it does not contain the trademarked... information, see the manual provided by the Weiderschain lab. Find Tet-pLKO-puro. lentiGuide-Puro - This...

  31. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

  32. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...microscopy (University of Amsterdam). He develops, characterizes and uses genetically encoded fluorescent probes...

  33. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...and David Root from the Broad Institute and Alex Chavez from the Wyss Institute were instrumental in helping...research and discovery, while also pushing us to challenge assumptions, holding us to nonprofit best practices...

  34. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...j.gendis.2017.04.001 Maetzig T, Galla M, Baum C, Schambach A. Gammaretroviral vectors: biology, technology...

  35. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...recombinase-dependent vectors, this can cause challenges for the experimental design as these vectors ...neurons reside.   Impact for scientists While the mechanisms by which retrograde AAV work are not yet understood...

  36. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...manipulations (CRISPR, etc.), and finish with the characterization of a function or phenotype in the surviving...significant survival advantage, such as a non-essential mechanism or any pathway with cellular redundancies, a screen...

  37. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  38. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...labeling. Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region...fusion proteins, such as Cas9 and Cas12a, creates challenges for in vivo delivery and cell engineering applications...

  40. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... in your professional circle to vouch for the character you painted in your personal statement. Make certain...hand to get you into that program of your dream.  “Chance favors the prepared mind” by Louis Pasteur These...
Showing: 521 - 560 of 792 results